Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria
This article aims to explore the antibacterial activity of thin films of TiO2 doped with Ag and Cu using two types of Gram-negative and Gram-positive test bacteria with clinical significance (Gracilicutes and Firmcutes bacteria). The thin films (thickness of about 60 nm) were deposited on glass subs...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2017-12-01
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| Series: | BioDiscovery |
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| Online Access: | https://biodiscovery.pensoft.net/article/21596/download/pdf/ |
| _version_ | 1818677903503654912 |
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| author | Dragomira Stoyanova Iliana Ivanova Orlin Angelov Todorka Vladkova |
| author_facet | Dragomira Stoyanova Iliana Ivanova Orlin Angelov Todorka Vladkova |
| author_sort | Dragomira Stoyanova |
| collection | DOAJ |
| description | This article aims to explore the antibacterial activity of thin films of TiO2 doped with Ag and Cu using two types of Gram-negative and Gram-positive test bacteria with clinical significance (Gracilicutes and Firmcutes bacteria). The thin films (thickness of about 60 nm) were deposited on glass substrates by radio frequency magnetron co-sputtering (r.f. power of 50 W) of TiO2 target with Ag and Cu pieces on its surface in an Ar atmosphere (0.8 Pa) without heating during the deposition. The total surface area of the Ag was 60 mm2 and that of the Cu was 100 mm2. Bacillus cereus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli and Pseudomonas sp. were used as test strains. The antibacterial actvity of the films was evaluated by the classical Koch's method and optical density measurements. The bactericidal effect was established at different time points between 30 min and 90 min for Pseudomonas sp. and S. enterica. The Firmicutes bacteria B. cereus and S. epidermidis were killed at the 4th and 8th hour of the treatment, respectively. The effect on E. coli was bacteriostatic until the 10th hour. The results were confirmed by assessment of the bacterial dehydrogenase activity. The studied thin films of TiO2 co-doped with Ag and Cu have a potential for application as antibacterial coatings. |
| first_indexed | 2024-12-17T09:06:46Z |
| format | Article |
| id | doaj.art-8d292c3fdf5147568bad921fe9fca258 |
| institution | Directory Open Access Journal |
| issn | 2050-2966 |
| language | English |
| last_indexed | 2024-12-17T09:06:46Z |
| publishDate | 2017-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | BioDiscovery |
| spelling | doaj.art-8d292c3fdf5147568bad921fe9fca2582022-12-21T21:55:26ZengPensoft PublishersBioDiscovery2050-29662017-12-012011410.3897/biodiscovery.20.e2159621596Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteriaDragomira Stoyanova0Iliana Ivanova1Orlin Angelov2Todorka Vladkova3Department of “General and Industrial Microbiology”, Faculty of Biology, Sofia University “St. Kl. Ohridski”Department of “General and Industrial Microbiology”, Faculty of Biology, Sofia University “St. Kl. Ohridski”Central Laboratory of Solar Energy and New Energy Sources, Bulgarian Academy of SciencesUniversity of Chemical Technology and MetallurgyThis article aims to explore the antibacterial activity of thin films of TiO2 doped with Ag and Cu using two types of Gram-negative and Gram-positive test bacteria with clinical significance (Gracilicutes and Firmcutes bacteria). The thin films (thickness of about 60 nm) were deposited on glass substrates by radio frequency magnetron co-sputtering (r.f. power of 50 W) of TiO2 target with Ag and Cu pieces on its surface in an Ar atmosphere (0.8 Pa) without heating during the deposition. The total surface area of the Ag was 60 mm2 and that of the Cu was 100 mm2. Bacillus cereus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli and Pseudomonas sp. were used as test strains. The antibacterial actvity of the films was evaluated by the classical Koch's method and optical density measurements. The bactericidal effect was established at different time points between 30 min and 90 min for Pseudomonas sp. and S. enterica. The Firmicutes bacteria B. cereus and S. epidermidis were killed at the 4th and 8th hour of the treatment, respectively. The effect on E. coli was bacteriostatic until the 10th hour. The results were confirmed by assessment of the bacterial dehydrogenase activity. The studied thin films of TiO2 co-doped with Ag and Cu have a potential for application as antibacterial coatings.https://biodiscovery.pensoft.net/article/21596/download/pdf/TiO<sub>2</sub> thin filmsbactericidal effect |
| spellingShingle | Dragomira Stoyanova Iliana Ivanova Orlin Angelov Todorka Vladkova Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria BioDiscovery TiO<sub>2</sub> thin films bactericidal effect |
| title | Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria |
| title_full | Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria |
| title_fullStr | Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria |
| title_full_unstemmed | Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria |
| title_short | Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria |
| title_sort | antibacterial activity of thin films tio2 doped with ag and cu on gracilicutes and firmicutes bacteria |
| topic | TiO<sub>2</sub> thin films bactericidal effect |
| url | https://biodiscovery.pensoft.net/article/21596/download/pdf/ |
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