Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing.
Pathogenic non-O1/non-O139 Vibrio cholerae strains can cause sporadic outbreaks of cholera worldwide. In this study, multilocus sequence typing (MLST) of seven housekeeping genes was applied to 55 non-O1/non-O139 isolates from clinical and environmental sources. Data from five published O1 isolates...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3679125?pdf=render |
| _version_ | 1811336589041729536 |
|---|---|
| author | Sophie Octavia Anna Salim Jacob Kurniawan Connie Lam Queenie Leung Sunjukta Ahsan Peter R Reeves G Balakrish Nair Ruiting Lan |
| author_facet | Sophie Octavia Anna Salim Jacob Kurniawan Connie Lam Queenie Leung Sunjukta Ahsan Peter R Reeves G Balakrish Nair Ruiting Lan |
| author_sort | Sophie Octavia |
| collection | DOAJ |
| description | Pathogenic non-O1/non-O139 Vibrio cholerae strains can cause sporadic outbreaks of cholera worldwide. In this study, multilocus sequence typing (MLST) of seven housekeeping genes was applied to 55 non-O1/non-O139 isolates from clinical and environmental sources. Data from five published O1 isolates and 17 genomes were also included, giving a total of 77 isolates available for analysis. There were 66 sequence types (STs), with the majority being unique, and only three clonal complexes. The V. cholerae strains can be divided into four subpopulations with evidence of recombination among the subpopulations. Subpopulations I and III contained predominantly clinical strains. PCR screening for virulence factors including Vibrio pathogenicity island (VPI), cholera toxin prophage (CTXΦ), type III secretion system (T3SS), and enterotoxin genes (rtxA and sto/stn) showed that combinations of these factors were present in the clinical isolates with 85.7% having rtxA, 51.4% T3SS, 31.4% VPI, 31.4% sto/stn (NAG-ST) and 11.4% CTXΦ. These factors were also present in environmental isolates but at a lower frequency. Five strains previously mis-identified as V. cholerae serogroups O114 to O117 were also analysed and formed a separate population with V. mimicus. The MLST scheme developed in this study provides a framework to identify sporadic cholera isolates by genetic identity. |
| first_indexed | 2024-04-13T17:41:37Z |
| format | Article |
| id | doaj.art-8d2c511da9b14cbeb5875619b71de4f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T17:41:37Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8d2c511da9b14cbeb5875619b71de4f72022-12-22T02:37:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6534210.1371/journal.pone.0065342Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing.Sophie OctaviaAnna SalimJacob KurniawanConnie LamQueenie LeungSunjukta AhsanPeter R ReevesG Balakrish NairRuiting LanPathogenic non-O1/non-O139 Vibrio cholerae strains can cause sporadic outbreaks of cholera worldwide. In this study, multilocus sequence typing (MLST) of seven housekeeping genes was applied to 55 non-O1/non-O139 isolates from clinical and environmental sources. Data from five published O1 isolates and 17 genomes were also included, giving a total of 77 isolates available for analysis. There were 66 sequence types (STs), with the majority being unique, and only three clonal complexes. The V. cholerae strains can be divided into four subpopulations with evidence of recombination among the subpopulations. Subpopulations I and III contained predominantly clinical strains. PCR screening for virulence factors including Vibrio pathogenicity island (VPI), cholera toxin prophage (CTXΦ), type III secretion system (T3SS), and enterotoxin genes (rtxA and sto/stn) showed that combinations of these factors were present in the clinical isolates with 85.7% having rtxA, 51.4% T3SS, 31.4% VPI, 31.4% sto/stn (NAG-ST) and 11.4% CTXΦ. These factors were also present in environmental isolates but at a lower frequency. Five strains previously mis-identified as V. cholerae serogroups O114 to O117 were also analysed and formed a separate population with V. mimicus. The MLST scheme developed in this study provides a framework to identify sporadic cholera isolates by genetic identity.http://europepmc.org/articles/PMC3679125?pdf=render |
| spellingShingle | Sophie Octavia Anna Salim Jacob Kurniawan Connie Lam Queenie Leung Sunjukta Ahsan Peter R Reeves G Balakrish Nair Ruiting Lan Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing. PLoS ONE |
| title | Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing. |
| title_full | Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing. |
| title_fullStr | Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing. |
| title_full_unstemmed | Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing. |
| title_short | Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing. |
| title_sort | population structure and evolution of non o1 non o139 vibrio cholerae by multilocus sequence typing |
| url | http://europepmc.org/articles/PMC3679125?pdf=render |
| work_keys_str_mv | AT sophieoctavia populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT annasalim populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT jacobkurniawan populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT connielam populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT queenieleung populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT sunjuktaahsan populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT peterrreeves populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT gbalakrishnair populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping AT ruitinglan populationstructureandevolutionofnono1nono139vibriocholeraebymultilocussequencetyping |