SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK
<span style="font-family: 'Times New Roman', serif; font-size: medium;"><span style="font-size: 16px; line-height: 18px;"><p>Strains of <em>S. aureus</em> were isolated from individual milk samples of 500 lacting cows from...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HACCP Consulting
2010-11-01
|
| Series: | Potravinarstvo |
| Subjects: | |
| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/21 |
| Summary: | <span style="font-family: 'Times New Roman', serif; font-size: medium;"><span style="font-size: 16px; line-height: 18px;"><p>Strains of <em>S. aureus</em> were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 - Košice region, 300 samples; PH 2 - Žilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus <em>Staphylococcus</em> isolated on Baird-Parker agar from milk samples was p < 0,0001<sup>(</sup><sup>***)</sup><sup> </sup>. Totally, 122 milk samples were positive for the presence of <em>Staphylococcus aureus</em> (83 Košice regions, 52 Žilina regions). All 122 isolates of <em>S. aureus</em> were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 <em>S. aureus</em> isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance <em>mec</em>A gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of <em>S. aureus</em> from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of <em>mec</em>A gene coding the methicilin resistance.</p><p> </p><p><span style="font-family: Verdana, Arial, Helvetica, sans-serif; line-height: normal; font-size: 12px;"><strong>doi:10.5219/21</strong></span></p> <p> </p> <br /></span></span> |
|---|---|
| ISSN: | 1338-0230 1337-0960 |