SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK
<span style="font-family: 'Times New Roman', serif; font-size: medium;"><span style="font-size: 16px; line-height: 18px;"><p>Strains of <em>S. aureus</em> were isolated from individual milk samples of 500 lacting cows from...
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| Format: | Article |
| Language: | English |
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HACCP Consulting
2010-11-01
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| Series: | Potravinarstvo |
| Subjects: | |
| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/21 |
| _version_ | 1818756993886715904 |
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| author | Eva Dudriková Jana Pukáčová Lucia Poľaková |
| author_facet | Eva Dudriková Jana Pukáčová Lucia Poľaková |
| author_sort | Eva Dudriková |
| collection | DOAJ |
| description | <span style="font-family: 'Times New Roman', serif; font-size: medium;"><span style="font-size: 16px; line-height: 18px;"><p>Strains of <em>S. aureus</em> were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 - Košice region, 300 samples; PH 2 - Žilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus <em>Staphylococcus</em> isolated on Baird-Parker agar from milk samples was p < 0,0001<sup>(</sup><sup>***)</sup><sup> </sup>. Totally, 122 milk samples were positive for the presence of <em>Staphylococcus aureus</em> (83 Košice regions, 52 Žilina regions). All 122 isolates of <em>S. aureus</em> were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 <em>S. aureus</em> isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance <em>mec</em>A gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of <em>S. aureus</em> from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of <em>mec</em>A gene coding the methicilin resistance.</p><p> </p><p><span style="font-family: Verdana, Arial, Helvetica, sans-serif; line-height: normal; font-size: 12px;"><strong>doi:10.5219/21</strong></span></p> <p> </p> <br /></span></span> |
| first_indexed | 2024-12-18T06:03:52Z |
| format | Article |
| id | doaj.art-8d2f8c31dbd64222b251a80f4b452f82 |
| institution | Directory Open Access Journal |
| issn | 1338-0230 1337-0960 |
| language | English |
| last_indexed | 2024-12-18T06:03:52Z |
| publishDate | 2010-11-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-8d2f8c31dbd64222b251a80f4b452f822022-12-21T21:18:37ZengHACCP ConsultingPotravinarstvo1338-02301337-09602010-11-0144566410.5219/21SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILKEva DudrikováJana PukáčováLucia Poľaková<span style="font-family: 'Times New Roman', serif; font-size: medium;"><span style="font-size: 16px; line-height: 18px;"><p>Strains of <em>S. aureus</em> were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 - Košice region, 300 samples; PH 2 - Žilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus <em>Staphylococcus</em> isolated on Baird-Parker agar from milk samples was p < 0,0001<sup>(</sup><sup>***)</sup><sup> </sup>. Totally, 122 milk samples were positive for the presence of <em>Staphylococcus aureus</em> (83 Košice regions, 52 Žilina regions). All 122 isolates of <em>S. aureus</em> were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 <em>S. aureus</em> isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance <em>mec</em>A gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of <em>S. aureus</em> from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of <em>mec</em>A gene coding the methicilin resistance.</p><p> </p><p><span style="font-family: Verdana, Arial, Helvetica, sans-serif; line-height: normal; font-size: 12px;"><strong>doi:10.5219/21</strong></span></p> <p> </p> <br /></span></span>http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/21milk, antibiotic resistance, mecA gene, PCR |
| spellingShingle | Eva Dudriková Jana Pukáčová Lucia Poľaková SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK Potravinarstvo milk, antibiotic resistance, mecA gene, PCR |
| title | SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK |
| title_full | SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK |
| title_fullStr | SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK |
| title_full_unstemmed | SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK |
| title_short | SENSITIVY TO ANTIBIOTICS IN STRAINS OF S. AUREUS ISOLATED FROM COW´S MILK |
| title_sort | sensitivy to antibiotics in strains of s aureus isolated from cow´s milk |
| topic | milk, antibiotic resistance, mecA gene, PCR |
| url | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/21 |
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