Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population
Parkinson’s disease (PD) is a complex neurodegenerative condition characterized by alpha-synuclein aggregation and dysfunctional protein degradation pathways. This study investigates the differential gene expression of pivotal components (UBE2K, PSMC4, SKP1, and HSPA8) within these pathways in a Mex...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-12-01
|
| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1298560/full |
| _version_ | 1797404585891987456 |
|---|---|
| author | Alma C. Salas-Leal Sergio M. Salas-Pacheco Erik I. Hernández-Cosaín Lilia M. Vélez-Vélez Elizabeth I. Antuna-Salcido Francisco X. Castellanos-Juárez Edna M. Méndez-Hernández Osmel La Llave-León Gerardo Quiñones-Canales Oscar Arias-Carrión Ada A. Sandoval-Carrillo José M. Salas-Pacheco |
| author_facet | Alma C. Salas-Leal Sergio M. Salas-Pacheco Erik I. Hernández-Cosaín Lilia M. Vélez-Vélez Elizabeth I. Antuna-Salcido Francisco X. Castellanos-Juárez Edna M. Méndez-Hernández Osmel La Llave-León Gerardo Quiñones-Canales Oscar Arias-Carrión Ada A. Sandoval-Carrillo José M. Salas-Pacheco |
| author_sort | Alma C. Salas-Leal |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is a complex neurodegenerative condition characterized by alpha-synuclein aggregation and dysfunctional protein degradation pathways. This study investigates the differential gene expression of pivotal components (UBE2K, PSMC4, SKP1, and HSPA8) within these pathways in a Mexican-Mestizo PD population compared to healthy controls. We enrolled 87 PD patients and 87 controls, assessing their gene expression levels via RT-qPCR. Our results reveal a significant downregulation of PSMC4, SKP1, and HSPA8 in the PD group (p = 0.033, p = 0.003, and p = 0.002, respectively). Logistic regression analyses establish a strong association between PD and reduced expression of PSMC4, SKP1, and HSPA8 (OR = 0.640, 95% CI = 0.415–0.987; OR = 0.000, 95% CI = 0.000–0.075; OR = 0.550, 95% CI = 0.368–0.823, respectively). Conversely, UBE2K exhibited no significant association or expression difference between the groups. Furthermore, we develop a gene expression model based on HSPA8, PSMC4, and SKP1, demonstrating robust discrimination between healthy controls and PD patients. Notably, the model’s diagnostic efficacy is particularly pronounced in early-stage PD. In conclusion, our study provides compelling evidence linking decreased gene expression of PSMC4, SKP1, and HSPA8 to PD in the Mexican-Mestizo population. Additionally, our gene expression model exhibits promise as a diagnostic tool, particularly for early-stage PD diagnosis. |
| first_indexed | 2024-03-09T02:57:09Z |
| format | Article |
| id | doaj.art-8d300725e5e948b6bf07ed8111dec9a1 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-03-09T02:57:09Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-8d300725e5e948b6bf07ed8111dec9a12023-12-05T04:24:46ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-12-011610.3389/fnmol.2023.12985601298560Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo populationAlma C. Salas-Leal0Sergio M. Salas-Pacheco1Erik I. Hernández-Cosaín2Lilia M. Vélez-Vélez3Elizabeth I. Antuna-Salcido4Francisco X. Castellanos-Juárez5Edna M. Méndez-Hernández6Osmel La Llave-León7Gerardo Quiñones-Canales8Oscar Arias-Carrión9Ada A. Sandoval-Carrillo10José M. Salas-Pacheco11Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoHospital General Santiago Ramón y Cajal-ISSSTE, Durango, MéxicoUnidad de Trastornos del Movimiento y Sueño, Hospital General Dr. Manuel Gea González, Ciudad de México, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoInstituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, MéxicoParkinson’s disease (PD) is a complex neurodegenerative condition characterized by alpha-synuclein aggregation and dysfunctional protein degradation pathways. This study investigates the differential gene expression of pivotal components (UBE2K, PSMC4, SKP1, and HSPA8) within these pathways in a Mexican-Mestizo PD population compared to healthy controls. We enrolled 87 PD patients and 87 controls, assessing their gene expression levels via RT-qPCR. Our results reveal a significant downregulation of PSMC4, SKP1, and HSPA8 in the PD group (p = 0.033, p = 0.003, and p = 0.002, respectively). Logistic regression analyses establish a strong association between PD and reduced expression of PSMC4, SKP1, and HSPA8 (OR = 0.640, 95% CI = 0.415–0.987; OR = 0.000, 95% CI = 0.000–0.075; OR = 0.550, 95% CI = 0.368–0.823, respectively). Conversely, UBE2K exhibited no significant association or expression difference between the groups. Furthermore, we develop a gene expression model based on HSPA8, PSMC4, and SKP1, demonstrating robust discrimination between healthy controls and PD patients. Notably, the model’s diagnostic efficacy is particularly pronounced in early-stage PD. In conclusion, our study provides compelling evidence linking decreased gene expression of PSMC4, SKP1, and HSPA8 to PD in the Mexican-Mestizo population. Additionally, our gene expression model exhibits promise as a diagnostic tool, particularly for early-stage PD diagnosis.https://www.frontiersin.org/articles/10.3389/fnmol.2023.1298560/fullParkinson’s diseaseUBE2KPSMC4SKP1HSPA8protein degradation systems |
| spellingShingle | Alma C. Salas-Leal Sergio M. Salas-Pacheco Erik I. Hernández-Cosaín Lilia M. Vélez-Vélez Elizabeth I. Antuna-Salcido Francisco X. Castellanos-Juárez Edna M. Méndez-Hernández Osmel La Llave-León Gerardo Quiñones-Canales Oscar Arias-Carrión Ada A. Sandoval-Carrillo José M. Salas-Pacheco Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population Frontiers in Molecular Neuroscience Parkinson’s disease UBE2K PSMC4 SKP1 HSPA8 protein degradation systems |
| title | Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population |
| title_full | Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population |
| title_fullStr | Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population |
| title_full_unstemmed | Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population |
| title_short | Differential expression of PSMC4, SKP1, and HSPA8 in Parkinson’s disease: insights from a Mexican mestizo population |
| title_sort | differential expression of psmc4 skp1 and hspa8 in parkinson s disease insights from a mexican mestizo population |
| topic | Parkinson’s disease UBE2K PSMC4 SKP1 HSPA8 protein degradation systems |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1298560/full |
| work_keys_str_mv | AT almacsalasleal differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT sergiomsalaspacheco differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT erikihernandezcosain differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT liliamvelezvelez differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT elizabethiantunasalcido differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT franciscoxcastellanosjuarez differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT ednammendezhernandez differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT osmellallaveleon differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT gerardoquinonescanales differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT oscarariascarrion differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT adaasandovalcarrillo differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation AT josemsalaspacheco differentialexpressionofpsmc4skp1andhspa8inparkinsonsdiseaseinsightsfromamexicanmestizopopulation |