Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice
Abstract Curcuma longa L. extract (CLE) exerts various biological functions including antioxidant, anti-inflammation, anticancer, and antiallergenic effects. However, its immune-enhancing capacity remains unclear. Therefore, the immune-enhancing effect of CLE was investigated in RAW 264.7 cells and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-02-01
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| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13765-024-00865-y |
| _version_ | 1797273473835335680 |
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| author | Se Jeong Kim San Kim Sehyeon Jang Da Hye Gu Jeong Min Park Jung A. Ryu Sung Ran Yoon Sung Keun Jung |
| author_facet | Se Jeong Kim San Kim Sehyeon Jang Da Hye Gu Jeong Min Park Jung A. Ryu Sung Ran Yoon Sung Keun Jung |
| author_sort | Se Jeong Kim |
| collection | DOAJ |
| description | Abstract Curcuma longa L. extract (CLE) exerts various biological functions including antioxidant, anti-inflammation, anticancer, and antiallergenic effects. However, its immune-enhancing capacity remains unclear. Therefore, the immune-enhancing effect of CLE was investigated in RAW 264.7 cells and cyclophosphamide (CPP)-induced immunosuppression model. CLE upregulated nitric oxide (NO) and reactive oxygen species production and increased inducible nitric oxide synthase and cyclooxygenase-2 expression without affecting the RAW 264.7 cells viability. The results of quantitative real-time reverse transcription polymerase chain reaction and sandwich enzyme-linked immunosorbent assay showed that CLE increased the gene expression and protein levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β in RAW 264.7 cells. Moreover, CLE upregulated p65, I kappa B kinase α/β, and I kappa B α (IκBα) phosphorylation and downregulated IκBα expression in RAW 264.7 cells. CLE also increased p65 translocation from the cytoplasmic to the nucleus in RAW 264.7 cells. The oral administration of CLE increased organ indexes (including the spleen and thymus) and NO production in peritoneal macrophages and improved natural killer cell activity in CPP-induced immunosuppression BALB/c mice. Overall, CLE could be a useful health functional food material that can improve innate immunity via macrophage activation. |
| first_indexed | 2024-03-07T14:44:52Z |
| format | Article |
| id | doaj.art-8d316aaa92b8408b88d05985560dde80 |
| institution | Directory Open Access Journal |
| issn | 2468-0842 |
| language | English |
| last_indexed | 2024-03-07T14:44:52Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-8d316aaa92b8408b88d05985560dde802024-03-05T20:03:07ZengSpringerOpenApplied Biological Chemistry2468-08422024-02-0167111210.1186/s13765-024-00865-yCurcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c miceSe Jeong Kim0San Kim1Sehyeon Jang2Da Hye Gu3Jeong Min Park4Jung A. Ryu5Sung Ran Yoon6Sung Keun Jung7School of Food Science and Biotechnology, Kyungpook National UniversitySchool of Food Science and Biotechnology, Kyungpook National UniversitySchool of Food Science and Biotechnology, Kyungpook National UniversitySchool of Food Science and Biotechnology, Kyungpook National UniversityDivision of Agricultural Environment Research, Gyeongsangbuk-Do Agricultural Research and Extension ServicesDivision of Agricultural Environment Research, Gyeongsangbuk-Do Agricultural Research and Extension ServicesDivision of Agricultural Environment Research, Gyeongsangbuk-Do Agricultural Research and Extension ServicesSchool of Food Science and Biotechnology, Kyungpook National UniversityAbstract Curcuma longa L. extract (CLE) exerts various biological functions including antioxidant, anti-inflammation, anticancer, and antiallergenic effects. However, its immune-enhancing capacity remains unclear. Therefore, the immune-enhancing effect of CLE was investigated in RAW 264.7 cells and cyclophosphamide (CPP)-induced immunosuppression model. CLE upregulated nitric oxide (NO) and reactive oxygen species production and increased inducible nitric oxide synthase and cyclooxygenase-2 expression without affecting the RAW 264.7 cells viability. The results of quantitative real-time reverse transcription polymerase chain reaction and sandwich enzyme-linked immunosorbent assay showed that CLE increased the gene expression and protein levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β in RAW 264.7 cells. Moreover, CLE upregulated p65, I kappa B kinase α/β, and I kappa B α (IκBα) phosphorylation and downregulated IκBα expression in RAW 264.7 cells. CLE also increased p65 translocation from the cytoplasmic to the nucleus in RAW 264.7 cells. The oral administration of CLE increased organ indexes (including the spleen and thymus) and NO production in peritoneal macrophages and improved natural killer cell activity in CPP-induced immunosuppression BALB/c mice. Overall, CLE could be a useful health functional food material that can improve innate immunity via macrophage activation.https://doi.org/10.1186/s13765-024-00865-yHealth functional foodImmune-enhancing effectInterleukin-1βInterleukin-6Natural killer cellNitric oxide |
| spellingShingle | Se Jeong Kim San Kim Sehyeon Jang Da Hye Gu Jeong Min Park Jung A. Ryu Sung Ran Yoon Sung Keun Jung Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice Applied Biological Chemistry Health functional food Immune-enhancing effect Interleukin-1β Interleukin-6 Natural killer cell Nitric oxide |
| title | Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice |
| title_full | Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice |
| title_fullStr | Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice |
| title_full_unstemmed | Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice |
| title_short | Curcuma longa L. extract increased immune responses in RAW 264.7 cells and cyclophosphamide-induced BALB/c mice |
| title_sort | curcuma longa l extract increased immune responses in raw 264 7 cells and cyclophosphamide induced balb c mice |
| topic | Health functional food Immune-enhancing effect Interleukin-1β Interleukin-6 Natural killer cell Nitric oxide |
| url | https://doi.org/10.1186/s13765-024-00865-y |
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