DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay
Introduction: Majority of the developmental delays in children are non-syndromic and they are believed to have an underlying DNA damage, though not well substantiated. Hence the present study was carried out to find out if there is any increased DNA damage in children with non-syndromic developm...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/7806/19578_CE(RA1)_F(T)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdf |
| Summary: | Introduction: Majority of the developmental delays in children
are non-syndromic and they are believed to have an underlying
DNA damage, though not well substantiated. Hence the present
study was carried out to find out if there is any increased DNA
damage in children with non-syndromic developmental delay
by using the comet assay.
Aim: The present case-control study was undertaken to assess
the level of DNA damage in children with non syndromic
developmental delay and compare the same with that of age
and sex matched controls using submarine gel electrophoresis
(Comet Assay).
Materials and Methods: The blood from clinically diagnosed
children with non syndromic developmental delay and controls
were subjected for alkaline version of comet assay – Single
cell gel electrophoresis using lymphocytes isolated from the
peripheral blood. The comets were observed under a bright field
microscope; photocaptured and scored using the Image J image
quantification software. Comet parameters were compared
between the cases and controls and statistical analysis and
interpretation of results was done using the statistical software
SPSS version 20.
Results: The mean comet tail length in cases and control was
20.77+7.659μm and 08.97+4.398μm respectively which was
statistically significant (p<0.001). Other comet parameters like
total comet length and % DNA in tail also showed a statistically
significant difference (p < 0.001) between cases and controls.
Conclusion: The current investigation unraveled increased levels
of DNA damage in children with non syndromic developmental
delay when compared to the controls. |
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| ISSN: | 2249-782X 0973-709X |