DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay
Introduction: Majority of the developmental delays in children are non-syndromic and they are believed to have an underlying DNA damage, though not well substantiated. Hence the present study was carried out to find out if there is any increased DNA damage in children with non-syndromic developm...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/7806/19578_CE(RA1)_F(T)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Surraj Susai Parkash Chand Vishnu Bhat Ballambattu Nandeesha Hanumanthappa Raveendranath Veeramani |
| author_facet | Surraj Susai Parkash Chand Vishnu Bhat Ballambattu Nandeesha Hanumanthappa Raveendranath Veeramani |
| author_sort | Surraj Susai |
| collection | DOAJ |
| description | Introduction: Majority of the developmental delays in children
are non-syndromic and they are believed to have an underlying
DNA damage, though not well substantiated. Hence the present
study was carried out to find out if there is any increased DNA
damage in children with non-syndromic developmental delay
by using the comet assay.
Aim: The present case-control study was undertaken to assess
the level of DNA damage in children with non syndromic
developmental delay and compare the same with that of age
and sex matched controls using submarine gel electrophoresis
(Comet Assay).
Materials and Methods: The blood from clinically diagnosed
children with non syndromic developmental delay and controls
were subjected for alkaline version of comet assay – Single
cell gel electrophoresis using lymphocytes isolated from the
peripheral blood. The comets were observed under a bright field
microscope; photocaptured and scored using the Image J image
quantification software. Comet parameters were compared
between the cases and controls and statistical analysis and
interpretation of results was done using the statistical software
SPSS version 20.
Results: The mean comet tail length in cases and control was
20.77+7.659μm and 08.97+4.398μm respectively which was
statistically significant (p<0.001). Other comet parameters like
total comet length and % DNA in tail also showed a statistically
significant difference (p < 0.001) between cases and controls.
Conclusion: The current investigation unraveled increased levels
of DNA damage in children with non syndromic developmental
delay when compared to the controls. |
| first_indexed | 2024-12-12T02:33:58Z |
| format | Article |
| id | doaj.art-8d386a0f45494f8b9a42b147cc6f6ccc |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T02:33:58Z |
| publishDate | 2016-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-8d386a0f45494f8b9a42b147cc6f6ccc2022-12-22T00:41:20ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-05-01105AC06AC0810.7860/JCDR/2016/19578.7806DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet AssaySurraj Susai0Parkash Chand1Vishnu Bhat Ballambattu2Nandeesha Hanumanthappa3Raveendranath Veeramani4Junior Resident, Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.Professor and Head, Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.Senior Professor and Head, Department of Neonatology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. Assistant Professor, Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.Assistant Professor, Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.Introduction: Majority of the developmental delays in children are non-syndromic and they are believed to have an underlying DNA damage, though not well substantiated. Hence the present study was carried out to find out if there is any increased DNA damage in children with non-syndromic developmental delay by using the comet assay. Aim: The present case-control study was undertaken to assess the level of DNA damage in children with non syndromic developmental delay and compare the same with that of age and sex matched controls using submarine gel electrophoresis (Comet Assay). Materials and Methods: The blood from clinically diagnosed children with non syndromic developmental delay and controls were subjected for alkaline version of comet assay – Single cell gel electrophoresis using lymphocytes isolated from the peripheral blood. The comets were observed under a bright field microscope; photocaptured and scored using the Image J image quantification software. Comet parameters were compared between the cases and controls and statistical analysis and interpretation of results was done using the statistical software SPSS version 20. Results: The mean comet tail length in cases and control was 20.77+7.659μm and 08.97+4.398μm respectively which was statistically significant (p<0.001). Other comet parameters like total comet length and % DNA in tail also showed a statistically significant difference (p < 0.001) between cases and controls. Conclusion: The current investigation unraveled increased levels of DNA damage in children with non syndromic developmental delay when compared to the controls.https://jcdr.net/articles/PDF/7806/19578_CE(RA1)_F(T)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdfdamage to deoxy-ribonucleic aciddelayed developmentgel electrophrosismalonyldialdehyde |
| spellingShingle | Surraj Susai Parkash Chand Vishnu Bhat Ballambattu Nandeesha Hanumanthappa Raveendranath Veeramani DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay Journal of Clinical and Diagnostic Research damage to deoxy-ribonucleic acid delayed development gel electrophrosis malonyldialdehyde |
| title | DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay |
| title_full | DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay |
| title_fullStr | DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay |
| title_full_unstemmed | DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay |
| title_short | DNA Damage Analysis in Children with Non-syndromic Developmental Delay by Comet Assay |
| title_sort | dna damage analysis in children with non syndromic developmental delay by comet assay |
| topic | damage to deoxy-ribonucleic acid delayed development gel electrophrosis malonyldialdehyde |
| url | https://jcdr.net/articles/PDF/7806/19578_CE(RA1)_F(T)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdf |
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