Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix
<i>Acarapis woodi</i> is a pathogen affecting honey bees health worldwide. Its prevalence may be underestimated due to the time-consuming traditional method for its diagnosis and the attitude in focusing the attention only onto <i>Varroa destructor</i>. New PCR techniques hav...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/6/2837 |
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| author | Marco Pietropaoli Silvia Tofani Giovanni Formato Roberta Carlotta Rubino Gabriele Pietrella Camilla Di Ruggiero Marcella Milito Carmine Merola Michele Amorena Antonella Cersini |
| author_facet | Marco Pietropaoli Silvia Tofani Giovanni Formato Roberta Carlotta Rubino Gabriele Pietrella Camilla Di Ruggiero Marcella Milito Carmine Merola Michele Amorena Antonella Cersini |
| author_sort | Marco Pietropaoli |
| collection | DOAJ |
| description | <i>Acarapis woodi</i> is a pathogen affecting honey bees health worldwide. Its prevalence may be underestimated due to the time-consuming traditional method for its diagnosis and the attitude in focusing the attention only onto <i>Varroa destructor</i>. New PCR techniques have allowed for the verification of the presence of <i>A. woodi</i> in 44 samples of honey bees and 11 samples of hive debris collected from 17 apiaries by the veterinary services of the Latium region (Central Italy). Overall, 9.1% of adult honey bee samples (all belonging to one apiary) and 6.3% of hive debris samples (belonging to 6 apiaries) were positive in an end point PCR and presence of the pathogen was confirmed through Sanger sequencing. Results demonstrated the potential underestimation of <i>A. woodi</i> occurrence in Italian apiaries and reported the first detection of <i>A. woodi</i> in hive debris samples. |
| first_indexed | 2024-03-09T20:09:51Z |
| format | Article |
| id | doaj.art-8d38866d54a94ff1a424710f52e51a59 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T20:09:51Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8d38866d54a94ff1a424710f52e51a592023-11-24T00:20:03ZengMDPI AGApplied Sciences2076-34172022-03-01126283710.3390/app12062837Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive MatrixMarco Pietropaoli0Silvia Tofani1Giovanni Formato2Roberta Carlotta Rubino3Gabriele Pietrella4Camilla Di Ruggiero5Marcella Milito6Carmine Merola7Michele Amorena8Antonella Cersini9Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, 00178 Rome, Italy<i>Acarapis woodi</i> is a pathogen affecting honey bees health worldwide. Its prevalence may be underestimated due to the time-consuming traditional method for its diagnosis and the attitude in focusing the attention only onto <i>Varroa destructor</i>. New PCR techniques have allowed for the verification of the presence of <i>A. woodi</i> in 44 samples of honey bees and 11 samples of hive debris collected from 17 apiaries by the veterinary services of the Latium region (Central Italy). Overall, 9.1% of adult honey bee samples (all belonging to one apiary) and 6.3% of hive debris samples (belonging to 6 apiaries) were positive in an end point PCR and presence of the pathogen was confirmed through Sanger sequencing. Results demonstrated the potential underestimation of <i>A. woodi</i> occurrence in Italian apiaries and reported the first detection of <i>A. woodi</i> in hive debris samples.https://www.mdpi.com/2076-3417/12/6/2837<i>Acarapis woodi</i>tracheal mite<i>Apis mellifera</i>hive debrisdiagnostic methodsItaly |
| spellingShingle | Marco Pietropaoli Silvia Tofani Giovanni Formato Roberta Carlotta Rubino Gabriele Pietrella Camilla Di Ruggiero Marcella Milito Carmine Merola Michele Amorena Antonella Cersini Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix Applied Sciences <i>Acarapis woodi</i> tracheal mite <i>Apis mellifera</i> hive debris diagnostic methods Italy |
| title | Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix |
| title_full | Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix |
| title_fullStr | Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix |
| title_full_unstemmed | Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix |
| title_short | Molecular Detection of <i>Acarapis woodi</i> Using Hive Debris as Innovative and Non-Invasive Matrix |
| title_sort | molecular detection of i acarapis woodi i using hive debris as innovative and non invasive matrix |
| topic | <i>Acarapis woodi</i> tracheal mite <i>Apis mellifera</i> hive debris diagnostic methods Italy |
| url | https://www.mdpi.com/2076-3417/12/6/2837 |
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