Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury)
The olfactory system of insects is important for behavioral activities as it recognizes internal and external volatile stimuli in the environment. Insect odorant degrading enzymes (ODEs), including antennal-specific carboxylesterases (CXEs), are known to degrade redundant odorant molecules or to hyd...
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PeerJ Inc.
2021-03-01
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| Online Access: | https://peerj.com/articles/10919.pdf |
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| author | Jia Ye Dingze Mang Ke Kang Cheng Chen Xiaoqing Zhang Yanping Tang Endang R. Purba Liwen Song Qing-He Zhang Longwa Zhang |
| author_facet | Jia Ye Dingze Mang Ke Kang Cheng Chen Xiaoqing Zhang Yanping Tang Endang R. Purba Liwen Song Qing-He Zhang Longwa Zhang |
| author_sort | Jia Ye |
| collection | DOAJ |
| description | The olfactory system of insects is important for behavioral activities as it recognizes internal and external volatile stimuli in the environment. Insect odorant degrading enzymes (ODEs), including antennal-specific carboxylesterases (CXEs), are known to degrade redundant odorant molecules or to hydrolyze important olfactory sex pheromone components and plant volatiles. Compared to many well-studied Type-I sex pheromone-producing lepidopteran species, the molecular mechanisms of the olfactory system of Type-II sex pheromone-producing Hyphantria cunea (Drury) remain poorly understood. In the current study, we first identified a total of ten CXE genes based on our previous H. unea antennal transcriptomic data. We constructed a phylogenetic tree to evaluate the relationship of HcunCXEs with other insects’ CXEs, and used quantitative PCR to investigate the gene expression of H. cunea CXEs (HcunCXEs). Our results indicate that HcunCXEs are highly expressed in antennae, legs and wings, suggesting a potential function in degrading sex pheromone components, host plant volatiles, and other xenobiotics. This study not only provides a theoretical basis for subsequent olfactory mechanism studies on H. cunea, but also offers some new insights into functions and evolutionary characteristics of CXEs in lepidopteran insects. From a practical point of view, these HcunCXEs might represent meaningful targets for developing behavioral interference control strategies against H. cunea. |
| first_indexed | 2024-03-09T06:40:22Z |
| format | Article |
| id | doaj.art-8d394c82754847a4a01c57e6ca3869a2 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:40:22Z |
| publishDate | 2021-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-8d394c82754847a4a01c57e6ca3869a22023-12-03T10:51:08ZengPeerJ Inc.PeerJ2167-83592021-03-019e1091910.7717/peerj.10919Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury)Jia Ye0Dingze Mang1Ke Kang2Cheng Chen3Xiaoqing Zhang4Yanping Tang5Endang R. Purba6Liwen Song7Qing-He Zhang8Longwa Zhang9Anhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, ChinaGraduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tyoko, JapanAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, ChinaAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, ChinaAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, ChinaAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, ChinaStructural Cellular Biology Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, JapanJilin Provincial Academy of Forestry Sciences, Changchun, ChinaSterling International, Inc., Spokane, WA, USAAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, ChinaThe olfactory system of insects is important for behavioral activities as it recognizes internal and external volatile stimuli in the environment. Insect odorant degrading enzymes (ODEs), including antennal-specific carboxylesterases (CXEs), are known to degrade redundant odorant molecules or to hydrolyze important olfactory sex pheromone components and plant volatiles. Compared to many well-studied Type-I sex pheromone-producing lepidopteran species, the molecular mechanisms of the olfactory system of Type-II sex pheromone-producing Hyphantria cunea (Drury) remain poorly understood. In the current study, we first identified a total of ten CXE genes based on our previous H. unea antennal transcriptomic data. We constructed a phylogenetic tree to evaluate the relationship of HcunCXEs with other insects’ CXEs, and used quantitative PCR to investigate the gene expression of H. cunea CXEs (HcunCXEs). Our results indicate that HcunCXEs are highly expressed in antennae, legs and wings, suggesting a potential function in degrading sex pheromone components, host plant volatiles, and other xenobiotics. This study not only provides a theoretical basis for subsequent olfactory mechanism studies on H. cunea, but also offers some new insights into functions and evolutionary characteristics of CXEs in lepidopteran insects. From a practical point of view, these HcunCXEs might represent meaningful targets for developing behavioral interference control strategies against H. cunea.https://peerj.com/articles/10919.pdf Hyphantria cunea (Drury) Olfactory system Carboxylesterases (CXEs) Expression pattern |
| spellingShingle | Jia Ye Dingze Mang Ke Kang Cheng Chen Xiaoqing Zhang Yanping Tang Endang R. Purba Liwen Song Qing-He Zhang Longwa Zhang Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury) PeerJ Hyphantria cunea (Drury) Olfactory system Carboxylesterases (CXEs) Expression pattern |
| title | Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury) |
| title_full | Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury) |
| title_fullStr | Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury) |
| title_full_unstemmed | Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury) |
| title_short | Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury) |
| title_sort | putative carboxylesterase gene identification and their expression patterns in hyphantria cunea drury |
| topic | Hyphantria cunea (Drury) Olfactory system Carboxylesterases (CXEs) Expression pattern |
| url | https://peerj.com/articles/10919.pdf |
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