A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines
Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs aga...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-10-01
|
| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0026 |
| _version_ | 1797660249528729600 |
|---|---|
| author | Yu Peng Chun'e Zhang Minyan Deng Haijuan Jiang Huishu Huang Yue Li Weiping Lai Yu-pin Lin Jun Yu |
| author_facet | Yu Peng Chun'e Zhang Minyan Deng Haijuan Jiang Huishu Huang Yue Li Weiping Lai Yu-pin Lin Jun Yu |
| author_sort | Yu Peng |
| collection | DOAJ |
| description | Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research. |
| first_indexed | 2024-03-11T18:28:03Z |
| format | Article |
| id | doaj.art-8d3956c1a1774b83b4e66b3d66f85710 |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-03-11T18:28:03Z |
| publishDate | 2023-10-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-8d3956c1a1774b83b4e66b3d66f857102023-10-13T14:54:43ZengFuture Science LtdBioTechniques0736-62051940-98182023-10-0175415015610.2144/btn-2023-0026A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokinesYu Peng0Chun'e Zhang1Minyan Deng2Haijuan Jiang3Huishu Huang4Yue Li5Weiping Lai6Yu-pin Lin7Jun Yu81Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, ChinaRecombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.https://www.future-science.com/doi/10.2144/btn-2023-0026affinitycell hybridizationELISArabbit recombinant antibodysurface plasmon resonance |
| spellingShingle | Yu Peng Chun'e Zhang Minyan Deng Haijuan Jiang Huishu Huang Yue Li Weiping Lai Yu-pin Lin Jun Yu A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines BioTechniques affinity cell hybridization ELISA rabbit recombinant antibody surface plasmon resonance |
| title | A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines |
| title_full | A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines |
| title_fullStr | A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines |
| title_full_unstemmed | A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines |
| title_short | A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines |
| title_sort | cell hybridization based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines |
| topic | affinity cell hybridization ELISA rabbit recombinant antibody surface plasmon resonance |
| url | https://www.future-science.com/doi/10.2144/btn-2023-0026 |
| work_keys_str_mv | AT yupeng acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT chunezhang acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT minyandeng acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT haijuanjiang acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT huishuhuang acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT yueli acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT weipinglai acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT yupinlin acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT junyu acellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT yupeng cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT chunezhang cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT minyandeng cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT haijuanjiang cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT huishuhuang cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT yueli cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT weipinglai cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT yupinlin cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines AT junyu cellhybridizationbasedmethodofgeneratingrecombinantrabbitmonoclonalantibodiesfordetectingcytokines |