Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects
Gas-chromatography-mass-spectrometry revealed the presence of various bioactive compounds with anticancer properties in protocorm-like-body (PLB) cultures of a Dendrobium hybrid orchid (Dendrobium Enopi x Dendrobium Pink Lady). Pre-illumination of red fluorescent light lessened the stimulating effec...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X2030271X |
| _version_ | 1818382939704000512 |
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| author | Lit Chow Yeow Bee Lynn Chew Subramaniam Sreeramanan |
| author_facet | Lit Chow Yeow Bee Lynn Chew Subramaniam Sreeramanan |
| author_sort | Lit Chow Yeow |
| collection | DOAJ |
| description | Gas-chromatography-mass-spectrometry revealed the presence of various bioactive compounds with anticancer properties in protocorm-like-body (PLB) cultures of a Dendrobium hybrid orchid (Dendrobium Enopi x Dendrobium Pink Lady). Pre-illumination of red fluorescent light lessened the stimulating effects of light-emitting diodes (LEDs) on secondary metabolites production among in vitro PLB cultures, possibly due to habituation. The highest flavonoid content of 16.79 μmol/ g of fresh weight (FW) was achieved under blue-red (1:1) LED for PLBs pre-treated with white LED for more than 3 subculture cycles. Phenolics content significantly reduced as PLBs pre-cultured under red fluorescent light for 2 subculture cycles were exposed to LED illuminations, where far red LED resulted in the lowest total phenolic content (18.85 μmol/ g FW). High intensity green LED (16.9 μmol/s) enhanced the accumulation of phenolics while amino acids such as L-leucine, glycine and proline exhibited no significant stimulating effect for secondary metabolites production. |
| first_indexed | 2024-12-14T02:58:26Z |
| format | Article |
| id | doaj.art-8d39615035ca46d384d97efde5f3ede1 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-14T02:58:26Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-8d39615035ca46d384d97efde5f3ede12022-12-21T23:19:35ZengElsevierBiotechnology Reports2215-017X2020-09-0127e00497Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effectsLit Chow Yeow0Bee Lynn Chew1Subramaniam Sreeramanan2School of Biological Sciences, Universiti Sains Malaysia, 11700 Gelugor, Pulau Pinang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, 11700 Gelugor, Pulau Pinang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, 11700 Gelugor, Pulau Pinang, Malaysia; Centre for Chemical Biology, Universiti Sains Malaysia, 11900 Bayan Lepas, Penang, Malaysia; Corresponding author at: School of Biological Sciences, Universiti Sains Malaysia, 11700 Gelugor, Pulau Pinang, Malaysia.Gas-chromatography-mass-spectrometry revealed the presence of various bioactive compounds with anticancer properties in protocorm-like-body (PLB) cultures of a Dendrobium hybrid orchid (Dendrobium Enopi x Dendrobium Pink Lady). Pre-illumination of red fluorescent light lessened the stimulating effects of light-emitting diodes (LEDs) on secondary metabolites production among in vitro PLB cultures, possibly due to habituation. The highest flavonoid content of 16.79 μmol/ g of fresh weight (FW) was achieved under blue-red (1:1) LED for PLBs pre-treated with white LED for more than 3 subculture cycles. Phenolics content significantly reduced as PLBs pre-cultured under red fluorescent light for 2 subculture cycles were exposed to LED illuminations, where far red LED resulted in the lowest total phenolic content (18.85 μmol/ g FW). High intensity green LED (16.9 μmol/s) enhanced the accumulation of phenolics while amino acids such as L-leucine, glycine and proline exhibited no significant stimulating effect for secondary metabolites production.http://www.sciencedirect.com/science/article/pii/S2215017X2030271XProtocorm-like-bodyBioactive compoundsPhenolicsHabituationLight-emitting diodes |
| spellingShingle | Lit Chow Yeow Bee Lynn Chew Subramaniam Sreeramanan Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects Biotechnology Reports Protocorm-like-body Bioactive compounds Phenolics Habituation Light-emitting diodes |
| title | Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects |
| title_full | Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects |
| title_fullStr | Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects |
| title_full_unstemmed | Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects |
| title_short | Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects |
| title_sort | elevation of secondary metabolites production through light emitting diodes leds illumination in protocorm like bodies plbs of dendrobium hybrid orchid rich in phytochemicals with therapeutic effects |
| topic | Protocorm-like-body Bioactive compounds Phenolics Habituation Light-emitting diodes |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X2030271X |
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