Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance
The increase in antimicrobial resistance has raised questions about how to use these drugs safely, especially in veterinary medicine, animal nutrition, and agriculture. <i>Escherichia coli</i> is an important human and animal pathogen that frequently contains plasmids carrying antibiotic...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/8/1509 |
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| author | Armando Hernández-Mendoza Rosalba Salgado-Morales Abimael Morán-Vázquez David López-Torres Blanca Inés García-Gómez Edgar Dantán-González |
| author_facet | Armando Hernández-Mendoza Rosalba Salgado-Morales Abimael Morán-Vázquez David López-Torres Blanca Inés García-Gómez Edgar Dantán-González |
| author_sort | Armando Hernández-Mendoza |
| collection | DOAJ |
| description | The increase in antimicrobial resistance has raised questions about how to use these drugs safely, especially in veterinary medicine, animal nutrition, and agriculture. <i>Escherichia coli</i> is an important human and animal pathogen that frequently contains plasmids carrying antibiotic resistance genes. Extra chromosomal elements are required for various functions or conditions in microorganisms. Several phage-like plasmids have been identified, which are important in antibiotic resistance. In this work, the molecular characterization of the pBOq-IncQ (4.5 kb) and pBOq-95LK (95 kb) plasmids found in the <i>E. coli</i> strain BOq 01, a multidrug resistant bacteria isolated from a poultry farm, are considered. Plasmid pBOq-IncQ belongs to the incQ incompatibility plasmid family and is involved in sulfonamide resistance. Plasmid pBOq-95LK is a lytic phage-like plasmid that is involved in the lysis of the <i>E. coli</i> BOq 01 strain and carries a bleomycin resistance gene and a strain cured of this plasmid shows bleomycin sensitivity. Induction of the lytic cycle indicates that this phage-like plasmid is an active phage. This type of plasmid has been reported to acquire genes such as <i>mcr-1</i>, which codes for colistin resistance and bacterial persistence and is a significant public health threat. A genome comparison, a pangenomic and phylogenomic analysis with other phage-like plasmids reported in the literature were performed to understand better the evolution of this kind of plasmid in bacteria and its potential importance in antibiotic resistance. |
| first_indexed | 2024-03-09T04:04:43Z |
| format | Article |
| id | doaj.art-8d3da268d1544154878d043e730d34db |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:04:43Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-8d3da268d1544154878d043e730d34db2023-12-03T14:08:52ZengMDPI AGMicroorganisms2076-26072022-07-01108150910.3390/microorganisms10081509Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic ResistanceArmando Hernández-Mendoza0Rosalba Salgado-Morales1Abimael Morán-Vázquez2David López-Torres3Blanca Inés García-Gómez4Edgar Dantán-González5Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca 62210, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca 62210, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca 62210, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca 62210, MexicoInstituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca 62210, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca 62210, MexicoThe increase in antimicrobial resistance has raised questions about how to use these drugs safely, especially in veterinary medicine, animal nutrition, and agriculture. <i>Escherichia coli</i> is an important human and animal pathogen that frequently contains plasmids carrying antibiotic resistance genes. Extra chromosomal elements are required for various functions or conditions in microorganisms. Several phage-like plasmids have been identified, which are important in antibiotic resistance. In this work, the molecular characterization of the pBOq-IncQ (4.5 kb) and pBOq-95LK (95 kb) plasmids found in the <i>E. coli</i> strain BOq 01, a multidrug resistant bacteria isolated from a poultry farm, are considered. Plasmid pBOq-IncQ belongs to the incQ incompatibility plasmid family and is involved in sulfonamide resistance. Plasmid pBOq-95LK is a lytic phage-like plasmid that is involved in the lysis of the <i>E. coli</i> BOq 01 strain and carries a bleomycin resistance gene and a strain cured of this plasmid shows bleomycin sensitivity. Induction of the lytic cycle indicates that this phage-like plasmid is an active phage. This type of plasmid has been reported to acquire genes such as <i>mcr-1</i>, which codes for colistin resistance and bacterial persistence and is a significant public health threat. A genome comparison, a pangenomic and phylogenomic analysis with other phage-like plasmids reported in the literature were performed to understand better the evolution of this kind of plasmid in bacteria and its potential importance in antibiotic resistance.https://www.mdpi.com/2076-2607/10/8/1509phage-likeantibiotic resistancelysogenicinsertion sequenceevolution |
| spellingShingle | Armando Hernández-Mendoza Rosalba Salgado-Morales Abimael Morán-Vázquez David López-Torres Blanca Inés García-Gómez Edgar Dantán-González Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance Microorganisms phage-like antibiotic resistance lysogenic insertion sequence evolution |
| title | Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance |
| title_full | Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance |
| title_fullStr | Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance |
| title_full_unstemmed | Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance |
| title_short | Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of <i>Escherichia coli</i> BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance |
| title_sort | molecular characterization of pboq incq and pboq 95lk plasmids of i escherichia coli i boq 01 a new isolated strain from poultry farming involved in antibiotic resistance |
| topic | phage-like antibiotic resistance lysogenic insertion sequence evolution |
| url | https://www.mdpi.com/2076-2607/10/8/1509 |
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