Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo
Lysozyme (Lyz) is an antimicrobial peptide, a safe adjunct, and it has been indicated that Lyz can promote vibrissae follicle growth by enhancing the hair-inductive capacity of dermal papilla cells in mice. The present study produced a new type of minoxidil (Mx)-coated antifungal Lyz-shelled microbu...
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| Format: | Article |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.668754/full |
| _version_ | 1818936650174038016 |
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| author | Ai-Ho Liao Ai-Ho Liao Yu-Jhen Huang Ho-Chiao Chuang Chih-Hung Wang Chih-Hung Wang Chih-Hung Wang Cheng-Ping Shih Chien-Ping Chiang Chien-Ping Chiang |
| author_facet | Ai-Ho Liao Ai-Ho Liao Yu-Jhen Huang Ho-Chiao Chuang Chih-Hung Wang Chih-Hung Wang Chih-Hung Wang Cheng-Ping Shih Chien-Ping Chiang Chien-Ping Chiang |
| author_sort | Ai-Ho Liao |
| collection | DOAJ |
| description | Lysozyme (Lyz) is an antimicrobial peptide, a safe adjunct, and it has been indicated that Lyz can promote vibrissae follicle growth by enhancing the hair-inductive capacity of dermal papilla cells in mice. The present study produced a new type of minoxidil (Mx)-coated antifungal Lyz-shelled microbubble (LyzMB) for inhibiting bacteria and allergies on the oily scalp. The potential of Mx-coated LyzMBs (Mx-LyzMBs) combined with ultrasound (US) and the role of LyzMB fragments in enhancing hair follicle growth were investigated. Mx grafted with LyzMBs were synthesized and the loading efficiency of Mx on cationic LyzMBs was 20.3%. The biological activity of Lyz in skin was determined using an activity assay kit and immunohistochemistry expression, and the activities in the US+Mx-LyzMBs group were 65.8 and 118.5 μU/mL at 6 and 18 h, respectively. In hair follicle cell culture experiments, the lengths of hair follicle cells were significantly enhanced in the US+Mx-LyzMBs group (108.2 ± 11.6 μm) compared to in the US+LyzMBs+Mx group (44.3 ± 9.8 μm) and the group with Mx alone (79.6 ± 12.0 μm) on day 2 (p < 0.001). During 21 days of treatment in animal experiments, the growth rates at days 10 and 14 in the US+Mx-LyzMBs group increased by 19.4 and 65.7%, respectively, and there were significant differences (p < 0.05) between the US+Mx-LyzMBs group and the other four groups. These findings indicate that 1-MHz US (applied at 3 W/cm2, acoustic pressure = 0.266 MPa) for 1 min combined with Mx-LyzMBs can significantly increase more penetration of Mx and LyzMB fragments into skin and enhance hair growth than Mx alone. |
| first_indexed | 2024-12-20T05:39:26Z |
| format | Article |
| id | doaj.art-8d3f120822d74c3eaa891d92cd8fa299 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-20T05:39:26Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8d3f120822d74c3eaa891d92cd8fa2992022-12-21T19:51:29ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-04-011210.3389/fphar.2021.668754668754Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In VivoAi-Ho Liao0Ai-Ho Liao1Yu-Jhen Huang2Ho-Chiao Chuang3Chih-Hung Wang4Chih-Hung Wang5Chih-Hung Wang6Cheng-Ping Shih7Chien-Ping Chiang8Chien-Ping Chiang9Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanDepartment of Biomedical Engineering, National Defense Medical Center, Taipei, TaiwanGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanDepartment of Mechanical Engineering, National Taipei University of Technology, Taipei, TaiwanGraduate Institute of Medical Sciences, National Defense Medical Center, Taipei, TaiwanDepartment of Otolaryngology–Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, TaiwanTaichung Armed Forces General Hospital, Taichung, TaiwanDepartment of Otolaryngology–Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, TaiwanDepartment of Dermatology, Tri-Service General Hospital, National Defense Medical Center, Taipei, TaiwanDepartment of Biochemistry, National Defense Medical Center, Taipei, TaiwanLysozyme (Lyz) is an antimicrobial peptide, a safe adjunct, and it has been indicated that Lyz can promote vibrissae follicle growth by enhancing the hair-inductive capacity of dermal papilla cells in mice. The present study produced a new type of minoxidil (Mx)-coated antifungal Lyz-shelled microbubble (LyzMB) for inhibiting bacteria and allergies on the oily scalp. The potential of Mx-coated LyzMBs (Mx-LyzMBs) combined with ultrasound (US) and the role of LyzMB fragments in enhancing hair follicle growth were investigated. Mx grafted with LyzMBs were synthesized and the loading efficiency of Mx on cationic LyzMBs was 20.3%. The biological activity of Lyz in skin was determined using an activity assay kit and immunohistochemistry expression, and the activities in the US+Mx-LyzMBs group were 65.8 and 118.5 μU/mL at 6 and 18 h, respectively. In hair follicle cell culture experiments, the lengths of hair follicle cells were significantly enhanced in the US+Mx-LyzMBs group (108.2 ± 11.6 μm) compared to in the US+LyzMBs+Mx group (44.3 ± 9.8 μm) and the group with Mx alone (79.6 ± 12.0 μm) on day 2 (p < 0.001). During 21 days of treatment in animal experiments, the growth rates at days 10 and 14 in the US+Mx-LyzMBs group increased by 19.4 and 65.7%, respectively, and there were significant differences (p < 0.05) between the US+Mx-LyzMBs group and the other four groups. These findings indicate that 1-MHz US (applied at 3 W/cm2, acoustic pressure = 0.266 MPa) for 1 min combined with Mx-LyzMBs can significantly increase more penetration of Mx and LyzMB fragments into skin and enhance hair growth than Mx alone.https://www.frontiersin.org/articles/10.3389/fphar.2021.668754/fullultrasoundcavitationlysozyme-shelled microbubblesminoxidilhair follicle |
| spellingShingle | Ai-Ho Liao Ai-Ho Liao Yu-Jhen Huang Ho-Chiao Chuang Chih-Hung Wang Chih-Hung Wang Chih-Hung Wang Cheng-Ping Shih Chien-Ping Chiang Chien-Ping Chiang Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo Frontiers in Pharmacology ultrasound cavitation lysozyme-shelled microbubbles minoxidil hair follicle |
| title | Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo |
| title_full | Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo |
| title_fullStr | Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo |
| title_full_unstemmed | Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo |
| title_short | Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo |
| title_sort | minoxidil coated lysozyme shelled microbubbes combined with ultrasound for the enhancement of hair follicle growth efficacy in vitro and in vivo |
| topic | ultrasound cavitation lysozyme-shelled microbubbles minoxidil hair follicle |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.668754/full |
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