The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase
Introduction: Production of extended-spectrum beta-lactamase (ESBL) is one of the most important resistance mechanisms. Today, community-acquired infections caused by ESBL-producing isolates are mentioned as well as nosocomial infections. Theaim of this study was to assess in vitro activity of vario...
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| Format: | Article |
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Bilimsel Tip Yayinevi
2015-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
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| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2015-20-02-092-098.pdf |
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| author | Şafak ÖZER BALİN Ayhan AKBULUT Affan DENK Ayşe SAĞMAK TARTAR Mehmet ÖZDEN Kudbettin DEMİRDAĞ |
| author_facet | Şafak ÖZER BALİN Ayhan AKBULUT Affan DENK Ayşe SAĞMAK TARTAR Mehmet ÖZDEN Kudbettin DEMİRDAĞ |
| author_sort | Şafak ÖZER BALİN |
| collection | DOAJ |
| description | Introduction: Production of extended-spectrum beta-lactamase (ESBL) is one of the most important resistance mechanisms. Today, community-acquired infections caused by ESBL-producing isolates are mentioned as well as nosocomial infections. Theaim of this study was to assess in vitro activity of various antimicrobials against 75 ESBL-producing Escherichia coli isolates obtained from urine samples which were determined in patients with the diagnosis of urinary tract infections.
Patients and Methods: In this study, all the 75 ESBL-producing E. Coli isolates obtained from urine samples between September 2011 and February 2012 were included. ESBL production of the isolates was detected by double disk synergy test. The minimum inhibitory concentrations (MICs) of antibiotics (ertapenem, imipenem, doripenem, and cefoperazone-sulbactam) were determined by broth microdilution method according to CLSI (Clinical and Laboratory Standards Institute) guidelines.
Results: While MIC50-MIC90 values were 0.125-0.25 μg/mL for ertapenem, 0.5-0.5 μg/mL for imipenem, and 0.06-0.06 μg/mL for
doripenem against nosocomial E. Coli isolates (n= 57), MIC50-MIC90 values were 0.06-0.125 μg/mL for ertapenem, 0.25-0.5 μg/mL for imipenem, and 0.06-0.06 μg/mL for doripenem against community-acquired E. Coli isolates (n= 18). Statistically, no difference was determined between these two groups (p> 0.05). Cefoperazone-sulbactam MIC50-MIC90 values were 8-64 μg/mL against both nosocomial and community-acquired E. Coli isolates.
Conclusion: Ertapenem, imipenem, and doripenem were more effective as in vitro than cefaperazone-sulbactam and they are
antimicrobials that could be used safely in the treatment of infections caused by ESBL-producing isolates. |
| first_indexed | 2024-04-10T13:35:49Z |
| format | Article |
| id | doaj.art-8d3f889a4721452b8bb5512732abc582 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T13:35:49Z |
| publishDate | 2015-06-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-8d3f889a4721452b8bb5512732abc5822023-02-15T16:11:23ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2015-06-012029298The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-LactamaseŞafak ÖZER BALİN0Ayhan AKBULUT1Affan DENK2Ayşe SAĞMAK TARTAR3Mehmet ÖZDEN4Kudbettin DEMİRDAĞ5Clinic of Infectious Diseases and Clinical Microbiology, Harput State Hospital, Elazig, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Fırat, Elazig, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Fırat, Elazig, TurkeyClinic of Infectious Diseases and Clinical Microbiology, Sirnak Cizre Dr. Sebahattin Cizrelioglu State Hospital, Sirnak, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Fırat, Elazig, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Fırat, Elazig, TurkeyIntroduction: Production of extended-spectrum beta-lactamase (ESBL) is one of the most important resistance mechanisms. Today, community-acquired infections caused by ESBL-producing isolates are mentioned as well as nosocomial infections. Theaim of this study was to assess in vitro activity of various antimicrobials against 75 ESBL-producing Escherichia coli isolates obtained from urine samples which were determined in patients with the diagnosis of urinary tract infections. Patients and Methods: In this study, all the 75 ESBL-producing E. Coli isolates obtained from urine samples between September 2011 and February 2012 were included. ESBL production of the isolates was detected by double disk synergy test. The minimum inhibitory concentrations (MICs) of antibiotics (ertapenem, imipenem, doripenem, and cefoperazone-sulbactam) were determined by broth microdilution method according to CLSI (Clinical and Laboratory Standards Institute) guidelines. Results: While MIC50-MIC90 values were 0.125-0.25 μg/mL for ertapenem, 0.5-0.5 μg/mL for imipenem, and 0.06-0.06 μg/mL for doripenem against nosocomial E. Coli isolates (n= 57), MIC50-MIC90 values were 0.06-0.125 μg/mL for ertapenem, 0.25-0.5 μg/mL for imipenem, and 0.06-0.06 μg/mL for doripenem against community-acquired E. Coli isolates (n= 18). Statistically, no difference was determined between these two groups (p> 0.05). Cefoperazone-sulbactam MIC50-MIC90 values were 8-64 μg/mL against both nosocomial and community-acquired E. Coli isolates. Conclusion: Ertapenem, imipenem, and doripenem were more effective as in vitro than cefaperazone-sulbactam and they are antimicrobials that could be used safely in the treatment of infections caused by ESBL-producing isolates.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2015-20-02-092-098.pdfErtapenemDoripenemImipenemCephoperazone-sulbactam |
| spellingShingle | Şafak ÖZER BALİN Ayhan AKBULUT Affan DENK Ayşe SAĞMAK TARTAR Mehmet ÖZDEN Kudbettin DEMİRDAĞ The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Ertapenem Doripenem Imipenem Cephoperazone-sulbactam |
| title | The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase |
| title_full | The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase |
| title_fullStr | The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase |
| title_full_unstemmed | The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase |
| title_short | The Comparison of Various Antibiotics Sensitivities in Uropathogen Escherichia coli Strains Producing Community and Hospital-Acquired Extended Spectrum Beta-Lactamase |
| title_sort | comparison of various antibiotics sensitivities in uropathogen escherichia coli strains producing community and hospital acquired extended spectrum beta lactamase |
| topic | Ertapenem Doripenem Imipenem Cephoperazone-sulbactam |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2015-20-02-092-098.pdf |
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