Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens.
Background:The emergence of horizontally transferable antibiotic resistance (AR) in probiotic strains of lactic acid bacteria (LAB) has incited a need to establish stringent safety measures for the assessment of commercial probiotics. Lactobacillus rhamnosusis not only a p...
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| Format: | Article |
| Language: | English |
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Advance Educational Institute & Research Centre
2020-12-01
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| Series: | International Journal of Endorsing Health Science Research |
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| Online Access: | http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/489/647 |
| _version_ | 1818881592223858688 |
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| author | Muffrah Hasan Afsheen Arif Abid Hasnain Tanveer Abbas |
| author_facet | Muffrah Hasan Afsheen Arif Abid Hasnain Tanveer Abbas |
| author_sort | Muffrah Hasan |
| collection | DOAJ |
| description | Background:The emergence of horizontally transferable antibiotic resistance (AR) in probiotic strains of lactic acid bacteria (LAB) has incited a need to establish stringent safety measures for the assessment of commercial probiotics. Lactobacillus rhamnosusis not only a prevalent constituent of commercially available probiotics but also has an abundant presence in fermented foods. Methodology:The present study tested antimicrobial activity and antibiotic susceptibility of L. rhamnosusisolated from fermented cabbage. Agar well diffusion assay was used to determine the antibacterial activity against a number of Gram-positive and Gram-negative bacteria, and antibiotic susceptibility testing was used to determine the antibiogram of several antibiotics. For this, the neutralized Cell-Free Supernatant (nCFS) of L.rhamnosuswas used, which exhibited antibacterial activity against several bacteria. Results:Antagonistic activity of nCFS of L.rhamnosuswas found against Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Salmonella typhimurium, Klebsiella pneumoniae, Salmonella enterica. Maximum inhibitory activity was observed against Listeria monocytogenes. Whereas, no activity was found against Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus subtilis, Vibrio alginolyticus, Proteus mirabilisand Proteus vulgaris. The intensity of inhibition among the isolates was in the sequence of Listeria monocytogenes> Escherichia coli> Bacillus cereus> Vibrio parahaemolyticus> Salmonella typhimurium. L.rhamnosuswas found susceptible against Chloramphenicol and Novobiocin. Moreover, it exhibited resistance to Amoxicillin, Levofloxacin, Oxacillin, Streptomycin and Tobramycin. Conclusion:In conclusion, this study suggests that the use of L.rhamnosusas a probiotic requires extensive examination of AR genes present in this strain. |
| first_indexed | 2024-12-19T15:04:19Z |
| format | Article |
| id | doaj.art-8d44bd615423417dbe5edf4263654de7 |
| institution | Directory Open Access Journal |
| issn | 2307-3748 2310-3841 |
| language | English |
| last_indexed | 2024-12-19T15:04:19Z |
| publishDate | 2020-12-01 |
| publisher | Advance Educational Institute & Research Centre |
| record_format | Article |
| series | International Journal of Endorsing Health Science Research |
| spelling | doaj.art-8d44bd615423417dbe5edf4263654de72022-12-21T20:16:29ZengAdvance Educational Institute & Research CentreInternational Journal of Endorsing Health Science Research2307-37482310-38412020-12-01914910.29052/IJEHSR.v9.i1.2021.04-09Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens.Muffrah Hasan0https://orcid.org/0000-0002-2569-346XAfsheen Arif1https://orcid.org/0000-0002-5681-0833Abid Hasnain2https://orcid.org/0000-0002-7390-0662Tanveer Abbas3https://orcid.org/0000-0002-1784-3077Department of Microbiology, University of Karachi, Karachi-Pakistan.Karachi Institute of Biotechnology and Genetic Engineering(KIBGE), University of Karachi, Karachi-Pakistan.Department of Food, Science &Technology, University of Karachi, Karachi-Pakistan.Department of Microbiology, University of Karachi, Karachi-Pakistan.Background:The emergence of horizontally transferable antibiotic resistance (AR) in probiotic strains of lactic acid bacteria (LAB) has incited a need to establish stringent safety measures for the assessment of commercial probiotics. Lactobacillus rhamnosusis not only a prevalent constituent of commercially available probiotics but also has an abundant presence in fermented foods. Methodology:The present study tested antimicrobial activity and antibiotic susceptibility of L. rhamnosusisolated from fermented cabbage. Agar well diffusion assay was used to determine the antibacterial activity against a number of Gram-positive and Gram-negative bacteria, and antibiotic susceptibility testing was used to determine the antibiogram of several antibiotics. For this, the neutralized Cell-Free Supernatant (nCFS) of L.rhamnosuswas used, which exhibited antibacterial activity against several bacteria. Results:Antagonistic activity of nCFS of L.rhamnosuswas found against Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Salmonella typhimurium, Klebsiella pneumoniae, Salmonella enterica. Maximum inhibitory activity was observed against Listeria monocytogenes. Whereas, no activity was found against Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus subtilis, Vibrio alginolyticus, Proteus mirabilisand Proteus vulgaris. The intensity of inhibition among the isolates was in the sequence of Listeria monocytogenes> Escherichia coli> Bacillus cereus> Vibrio parahaemolyticus> Salmonella typhimurium. L.rhamnosuswas found susceptible against Chloramphenicol and Novobiocin. Moreover, it exhibited resistance to Amoxicillin, Levofloxacin, Oxacillin, Streptomycin and Tobramycin. Conclusion:In conclusion, this study suggests that the use of L.rhamnosusas a probiotic requires extensive examination of AR genes present in this strain.http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/489/647antibacterial activitylactic acid bacteriaantibiotic susceptibilitylactobacillus rhamnosus |
| spellingShingle | Muffrah Hasan Afsheen Arif Abid Hasnain Tanveer Abbas Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens. International Journal of Endorsing Health Science Research antibacterial activity lactic acid bacteria antibiotic susceptibility lactobacillus rhamnosus |
| title | Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens. |
| title_full | Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens. |
| title_fullStr | Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens. |
| title_full_unstemmed | Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens. |
| title_short | Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosusMT539286 against Foodborne and Clinical pathogens. |
| title_sort | antibiotic susceptibility and antibacterial activity of neutralized cell free supernatant of lactobacillus rhamnosusmt539286 against foodborne and clinical pathogens |
| topic | antibacterial activity lactic acid bacteria antibiotic susceptibility lactobacillus rhamnosus |
| url | http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/489/647 |
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