A lipid-soluble antioxidant from polyallomer centrifuge tubes
Santowhite, a commercial antioxidant used in the manufacture of polypropylene, contaminates 12-ml polyallomer tubes to the extent of 0.2–0.3 mg/tube. It is distributed through the plastic and appears as a microscopic dust on the tubes' surfaces. In the preparation of polysomes from rat liver by...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1974-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520368073 |
| _version_ | 1819076417078427648 |
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| author | Clyde E. Opliger Peter C. Heinrich Robert E. Olson |
| author_facet | Clyde E. Opliger Peter C. Heinrich Robert E. Olson |
| author_sort | Clyde E. Opliger |
| collection | DOAJ |
| description | Santowhite, a commercial antioxidant used in the manufacture of polypropylene, contaminates 12-ml polyallomer tubes to the extent of 0.2–0.3 mg/tube. It is distributed through the plastic and appears as a microscopic dust on the tubes' surfaces. In the preparation of polysomes from rat liver by standard methods, approximately 15% of the antioxidant in previously water-washed tubes was removed by suspension of the polysome pellet with stirring in 1–2 ml of Tris buffer, pH 7.8. The polyallomer impurity has been shown to be identical with Santowhite, which is 4,4′-butylidene-bis(6-tert-butyl-m-cresol), by UV, mass, and NMR spectra. It is not uniformly removed from polyallomer tubes by common detergents but is removed by washing with acetone, to which the plastic is resistant. |
| first_indexed | 2024-12-21T18:40:58Z |
| format | Article |
| id | doaj.art-8d4945a5a3884270aa9d01e5fb847d6d |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:40:58Z |
| publishDate | 1974-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-8d4945a5a3884270aa9d01e5fb847d6d2022-12-21T18:54:00ZengElsevierJournal of Lipid Research0022-22751974-05-01153281283A lipid-soluble antioxidant from polyallomer centrifuge tubesClyde E. Opliger0Peter C. Heinrich1Robert E. Olson2Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104 and; Department of Vitamin and Nutrition Research, The Hoffmann-La Roche Laboratories, Basel, CH 4002, SwitzerlandDepartment of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104 and; Department of Vitamin and Nutrition Research, The Hoffmann-La Roche Laboratories, Basel, CH 4002, SwitzerlandDepartment of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104 and; Department of Vitamin and Nutrition Research, The Hoffmann-La Roche Laboratories, Basel, CH 4002, SwitzerlandSantowhite, a commercial antioxidant used in the manufacture of polypropylene, contaminates 12-ml polyallomer tubes to the extent of 0.2–0.3 mg/tube. It is distributed through the plastic and appears as a microscopic dust on the tubes' surfaces. In the preparation of polysomes from rat liver by standard methods, approximately 15% of the antioxidant in previously water-washed tubes was removed by suspension of the polysome pellet with stirring in 1–2 ml of Tris buffer, pH 7.8. The polyallomer impurity has been shown to be identical with Santowhite, which is 4,4′-butylidene-bis(6-tert-butyl-m-cresol), by UV, mass, and NMR spectra. It is not uniformly removed from polyallomer tubes by common detergents but is removed by washing with acetone, to which the plastic is resistant.http://www.sciencedirect.com/science/article/pii/S0022227520368073polysomesSantowhitelipid analysis |
| spellingShingle | Clyde E. Opliger Peter C. Heinrich Robert E. Olson A lipid-soluble antioxidant from polyallomer centrifuge tubes Journal of Lipid Research polysomes Santowhite lipid analysis |
| title | A lipid-soluble antioxidant from polyallomer centrifuge tubes |
| title_full | A lipid-soluble antioxidant from polyallomer centrifuge tubes |
| title_fullStr | A lipid-soluble antioxidant from polyallomer centrifuge tubes |
| title_full_unstemmed | A lipid-soluble antioxidant from polyallomer centrifuge tubes |
| title_short | A lipid-soluble antioxidant from polyallomer centrifuge tubes |
| title_sort | lipid soluble antioxidant from polyallomer centrifuge tubes |
| topic | polysomes Santowhite lipid analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520368073 |
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