The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study
Background: This study aimed to compare the antibacterial effects of calcium hydroxide, curcumin, and Aloe vera as an intracanal medicament on 6-week-old Enterococcus faecalis biofilm. Materials and Methods: In this in vitro study, the solution containing E. faecalis ATCC® 29212™ was inserted into t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Dental Research Journal |
| Subjects: | |
| Online Access: | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2022;volume=19;issue=1;spage=14;epage=14;aulast=Eskandarinezhad |
| _version_ | 1828427604351254528 |
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| author | Mahsa Eskandarinezhad Mohammad Hossein Soroush Barhaghi Kimia Allameh Amirhouman Sadrhaghighi Katayoun Katebi |
| author_facet | Mahsa Eskandarinezhad Mohammad Hossein Soroush Barhaghi Kimia Allameh Amirhouman Sadrhaghighi Katayoun Katebi |
| author_sort | Mahsa Eskandarinezhad |
| collection | DOAJ |
| description | Background: This study aimed to compare the antibacterial effects of calcium hydroxide, curcumin, and Aloe vera as an intracanal medicament on 6-week-old Enterococcus faecalis biofilm.
Materials and Methods: In this in vitro study, the solution containing E. faecalis ATCC® 29212™ was inserted into the canals of 72 single-rooted teeth to produce biofilm. The samples were divided into four groups, and the antibacterial agent as an intracanal drug was used for 1 week. Calcium hydroxide, curcumin, and A. vera were used as intracanal medicaments in three groups, respectively, and the fourth group was irrigated with normal saline. The collected debris was cultured by spread plate method for the bacterial count by colony count machine, and the number of bacteria in each sample per ml was reported in colony-forming unit per ml (CFU/ml). The data were analyzed using SPSS software. KruskalWallis and MannWhitney U-tests were used for comparison of CFU/ml between the study groups. P <0.05 was considered significant.
Results: The mean CFU/ml in the groups of calcium hydroxide, curcumin, and A. vera were 749.44, 630.55, and 1529.16, respectively. Compared with the control group, curcumin, calcium hydroxide, and A. vera showed 99.5%, 99.41%, and 98.79% antimicrobial effects, respectively. All three groups were significantly effective than the control group (P = 0.023, P = 0.023, and P = 0.024, respectively) but were not significantly different from each other (P = 0.057).
Conclusion: All three groups showed significant antibacterial activity compared to the control group, curcumin had the most significant effect, followed by calcium hydroxide and A. vera. Therefore, herbal materials can be considered safe alternatives to synthetic medicaments for intracanal usage. |
| first_indexed | 2024-12-10T17:05:10Z |
| format | Article |
| id | doaj.art-8d49ce08db8d4cc8bcfc079aebb38a97 |
| institution | Directory Open Access Journal |
| issn | 1735-3327 2008-0255 |
| language | English |
| last_indexed | 2024-12-10T17:05:10Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Dental Research Journal |
| spelling | doaj.art-8d49ce08db8d4cc8bcfc079aebb38a972022-12-22T01:40:29ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552022-01-01191141410.4103/1735-3327.338777The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro studyMahsa EskandarinezhadMohammad Hossein Soroush BarhaghiKimia AllamehAmirhouman SadrhaghighiKatayoun KatebiBackground: This study aimed to compare the antibacterial effects of calcium hydroxide, curcumin, and Aloe vera as an intracanal medicament on 6-week-old Enterococcus faecalis biofilm. Materials and Methods: In this in vitro study, the solution containing E. faecalis ATCC® 29212™ was inserted into the canals of 72 single-rooted teeth to produce biofilm. The samples were divided into four groups, and the antibacterial agent as an intracanal drug was used for 1 week. Calcium hydroxide, curcumin, and A. vera were used as intracanal medicaments in three groups, respectively, and the fourth group was irrigated with normal saline. The collected debris was cultured by spread plate method for the bacterial count by colony count machine, and the number of bacteria in each sample per ml was reported in colony-forming unit per ml (CFU/ml). The data were analyzed using SPSS software. KruskalWallis and MannWhitney U-tests were used for comparison of CFU/ml between the study groups. P <0.05 was considered significant. Results: The mean CFU/ml in the groups of calcium hydroxide, curcumin, and A. vera were 749.44, 630.55, and 1529.16, respectively. Compared with the control group, curcumin, calcium hydroxide, and A. vera showed 99.5%, 99.41%, and 98.79% antimicrobial effects, respectively. All three groups were significantly effective than the control group (P = 0.023, P = 0.023, and P = 0.024, respectively) but were not significantly different from each other (P = 0.057). Conclusion: All three groups showed significant antibacterial activity compared to the control group, curcumin had the most significant effect, followed by calcium hydroxide and A. vera. Therefore, herbal materials can be considered safe alternatives to synthetic medicaments for intracanal usage.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2022;volume=19;issue=1;spage=14;epage=14;aulast=Eskandarinezhadaloebiofilmscalcium hydroxidecurcuminenterococcus faecalis |
| spellingShingle | Mahsa Eskandarinezhad Mohammad Hossein Soroush Barhaghi Kimia Allameh Amirhouman Sadrhaghighi Katayoun Katebi The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study Dental Research Journal aloe biofilms calcium hydroxide curcumin enterococcus faecalis |
| title | The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study |
| title_full | The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study |
| title_fullStr | The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study |
| title_full_unstemmed | The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study |
| title_short | The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study |
| title_sort | comparison of calcium hydroxide curcumin and aloe vera antibacterial effects on 6 week old enterococcus faecalis biofilm as an intracanal medicament an in vitro study |
| topic | aloe biofilms calcium hydroxide curcumin enterococcus faecalis |
| url | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2022;volume=19;issue=1;spage=14;epage=14;aulast=Eskandarinezhad |
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