Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia
Acute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools are required...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/8/10/466 |
| _version_ | 1797572122295402496 |
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| author | Paula Calderon-Ruiz Gregor Haist Annina Mascus Andres F. Holguin-Rocha Philip Koliopoulos Tim Daniel Gabriel Velez Berlin Londono-Renteria Britta Gröndahl Alberto Tobon-Castano Stephan Gehring |
| author_facet | Paula Calderon-Ruiz Gregor Haist Annina Mascus Andres F. Holguin-Rocha Philip Koliopoulos Tim Daniel Gabriel Velez Berlin Londono-Renteria Britta Gröndahl Alberto Tobon-Castano Stephan Gehring |
| author_sort | Paula Calderon-Ruiz |
| collection | DOAJ |
| description | Acute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools are required to identify the causative agents. A descriptive cross-sectional study was conducted to evaluate the circulation and differential diagnosis of six pathogens in two regions of Colombia. The results obtained via multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) were comparable to those obtained using rapid tests conducted at the time of patient enrollment. Of 155 patients evaluated, 25 (16.1%) and 16 (10.3%) were positive for malaria and dengue, respectively; no samples were positive for any of the other infectious agents tested. In most cases, m-RT-PCR-ELISA confirmed the results previously obtained through rapid testing. |
| first_indexed | 2024-03-10T20:50:37Z |
| format | Article |
| id | doaj.art-8d4e1f5ead6d45b39d263de183faac34 |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-10T20:50:37Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-8d4e1f5ead6d45b39d263de183faac342023-11-19T18:23:02ZengMDPI AGTropical Medicine and Infectious Disease2414-63662023-10-0181046610.3390/tropicalmed8100466Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of ColombiaPaula Calderon-Ruiz0Gregor Haist1Annina Mascus2Andres F. Holguin-Rocha3Philip Koliopoulos4Tim Daniel5Gabriel Velez6Berlin Londono-Renteria7Britta Gröndahl8Alberto Tobon-Castano9Stephan Gehring10Center of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyCenter of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyCenter of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyDepartment of Entomology, College of Agriculture, Kansas State University, Manhattan, KS 66506, USACenter of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyCenter of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyMalaria Group, Lab 610, Faculty of Medicine, University of Antioquia, Medellin 050010, ColombiaSchool of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USACenter of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyMalaria Group, Lab 610, Faculty of Medicine, University of Antioquia, Medellin 050010, ColombiaCenter of Pediatric and Adolescent Medicine, University Medical Center, 55131 Mainz, GermanyAcute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools are required to identify the causative agents. A descriptive cross-sectional study was conducted to evaluate the circulation and differential diagnosis of six pathogens in two regions of Colombia. The results obtained via multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) were comparable to those obtained using rapid tests conducted at the time of patient enrollment. Of 155 patients evaluated, 25 (16.1%) and 16 (10.3%) were positive for malaria and dengue, respectively; no samples were positive for any of the other infectious agents tested. In most cases, m-RT-PCR-ELISA confirmed the results previously obtained through rapid testing.https://www.mdpi.com/2414-6366/8/10/466acute febrile syndromeColombiamalariadenguerapid testsmultiplex RT-PCR-ELISA |
| spellingShingle | Paula Calderon-Ruiz Gregor Haist Annina Mascus Andres F. Holguin-Rocha Philip Koliopoulos Tim Daniel Gabriel Velez Berlin Londono-Renteria Britta Gröndahl Alberto Tobon-Castano Stephan Gehring Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia Tropical Medicine and Infectious Disease acute febrile syndrome Colombia malaria dengue rapid tests multiplex RT-PCR-ELISA |
| title | Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_full | Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_fullStr | Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_full_unstemmed | Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_short | Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_sort | multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay screening for arbovirus and parasitic infections in febrile patients living in endemic regions of colombia |
| topic | acute febrile syndrome Colombia malaria dengue rapid tests multiplex RT-PCR-ELISA |
| url | https://www.mdpi.com/2414-6366/8/10/466 |
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