Agrochemical control of gene expression using evolved split RNA polymerase
Chemically-inducible gene expression systems are valuable tools for rational control of gene expression both for basic research and biotechnology. However, most chemical inducers are confined to certain groups of organisms. Therefore, dissecting interactions between different organisms could be chal...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2022-06-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/13619.pdf |
| _version_ | 1797419018420748288 |
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| author | Yuan Yuan Jin Miao |
| author_facet | Yuan Yuan Jin Miao |
| author_sort | Yuan Yuan |
| collection | DOAJ |
| description | Chemically-inducible gene expression systems are valuable tools for rational control of gene expression both for basic research and biotechnology. However, most chemical inducers are confined to certain groups of organisms. Therefore, dissecting interactions between different organisms could be challenging using existing chemically-inducible systems. We engineered a mandipropamid-induced gene expression system (Mandi-T7) based on evolved split T7 RNAP system. As a proof-of-principle, we induced GFP expression in E. coli cells grown inside plant tissue. |
| first_indexed | 2024-03-09T06:41:27Z |
| format | Article |
| id | doaj.art-8d55190af24d4f52a914c942dc5b5ea5 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:41:27Z |
| publishDate | 2022-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-8d55190af24d4f52a914c942dc5b5ea52023-12-03T10:49:45ZengPeerJ Inc.PeerJ2167-83592022-06-0110e1361910.7717/peerj.13619Agrochemical control of gene expression using evolved split RNA polymeraseYuan Yuan0Jin Miao1Department of Neurophysiology and Neuropharmacology, Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, ChinaDuke Kunshan University, Kunshan, ChinaChemically-inducible gene expression systems are valuable tools for rational control of gene expression both for basic research and biotechnology. However, most chemical inducers are confined to certain groups of organisms. Therefore, dissecting interactions between different organisms could be challenging using existing chemically-inducible systems. We engineered a mandipropamid-induced gene expression system (Mandi-T7) based on evolved split T7 RNAP system. As a proof-of-principle, we induced GFP expression in E. coli cells grown inside plant tissue.https://peerj.com/articles/13619.pdfInducible gene expressionT7 RNAPMandipropamid |
| spellingShingle | Yuan Yuan Jin Miao Agrochemical control of gene expression using evolved split RNA polymerase PeerJ Inducible gene expression T7 RNAP Mandipropamid |
| title | Agrochemical control of gene expression using evolved split RNA polymerase |
| title_full | Agrochemical control of gene expression using evolved split RNA polymerase |
| title_fullStr | Agrochemical control of gene expression using evolved split RNA polymerase |
| title_full_unstemmed | Agrochemical control of gene expression using evolved split RNA polymerase |
| title_short | Agrochemical control of gene expression using evolved split RNA polymerase |
| title_sort | agrochemical control of gene expression using evolved split rna polymerase |
| topic | Inducible gene expression T7 RNAP Mandipropamid |
| url | https://peerj.com/articles/13619.pdf |
| work_keys_str_mv | AT yuanyuan agrochemicalcontrolofgeneexpressionusingevolvedsplitrnapolymerase AT jinmiao agrochemicalcontrolofgeneexpressionusingevolvedsplitrnapolymerase |