MicroRNA-9 controls dendritic development by targeting REST
MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/02755 |
| _version_ | 1811236952075141120 |
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| author | Sebastian A Giusti Annette M Vogl Marisa M Brockmann Claudia A Vercelli Martin L Rein Dietrich Trümbach Wolfgang Wurst Demian Cazalla Valentin Stein Jan M Deussing Damian Refojo |
| author_facet | Sebastian A Giusti Annette M Vogl Marisa M Brockmann Claudia A Vercelli Martin L Rein Dietrich Trümbach Wolfgang Wurst Demian Cazalla Valentin Stein Jan M Deussing Damian Refojo |
| author_sort | Sebastian A Giusti |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth. |
| first_indexed | 2024-04-12T12:17:56Z |
| format | Article |
| id | doaj.art-8d59cdbcf0d342a4a0ce2238b26225f7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:17:56Z |
| publishDate | 2014-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8d59cdbcf0d342a4a0ce2238b26225f72022-12-22T03:33:23ZengeLife Sciences Publications LtdeLife2050-084X2014-11-01310.7554/eLife.02755MicroRNA-9 controls dendritic development by targeting RESTSebastian A Giusti0Annette M Vogl1Marisa M Brockmann2Claudia A Vercelli3Martin L Rein4Dietrich Trümbach5Wolfgang Wurst6Demian Cazalla7Valentin Stein8Jan M Deussing9Damian Refojo10Department of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, GermanyDepartment of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, GermanyDepartment of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany; Institute of Physiology, University of Bonn, Bonn, GermanyDepartment of Molecular Neurobiology, Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA)-CONICET-Partner Institute of the Max Planck Society, Buenos Aires, ArgentinaDepartment of Neurobiology of Stress and Neurogenetics, Max Planck Institute of Psychiatry, Munich, GermanyInstitute of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, GermanyInstitute of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany; Technische Universität, Munich, Germany; Deutsches Zentrum für Neurodegenerative Erkrankungen, Munich, Germany; Munich Cluster for System Neurology, Munich, GermanyDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, United StatesInstitute of Physiology, University of Bonn, Bonn, GermanyDepartment of Neurobiology of Stress and Neurogenetics, Max Planck Institute of Psychiatry, Munich, GermanyDepartment of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, GermanyMicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.https://elifesciences.org/articles/02755miR-9miR-9 spongemiR-9 reporterdendrite developmentREST |
| spellingShingle | Sebastian A Giusti Annette M Vogl Marisa M Brockmann Claudia A Vercelli Martin L Rein Dietrich Trümbach Wolfgang Wurst Demian Cazalla Valentin Stein Jan M Deussing Damian Refojo MicroRNA-9 controls dendritic development by targeting REST eLife miR-9 miR-9 sponge miR-9 reporter dendrite development REST |
| title | MicroRNA-9 controls dendritic development by targeting REST |
| title_full | MicroRNA-9 controls dendritic development by targeting REST |
| title_fullStr | MicroRNA-9 controls dendritic development by targeting REST |
| title_full_unstemmed | MicroRNA-9 controls dendritic development by targeting REST |
| title_short | MicroRNA-9 controls dendritic development by targeting REST |
| title_sort | microrna 9 controls dendritic development by targeting rest |
| topic | miR-9 miR-9 sponge miR-9 reporter dendrite development REST |
| url | https://elifesciences.org/articles/02755 |
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