Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism
Abstract The use of non-drug intervention for calcium deficiency has attracted attention in recent years. Although calcium carbonate is the preferred raw material for calcium supplementation, there are few reports on the mechanism of the combined action of chondroitin sulfate and calcium to alleviat...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-02-01
|
| Series: | Nutrition & Metabolism |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12986-023-00726-3 |
| _version_ | 1811166071021895680 |
|---|---|
| author | Tianshu Liu Hai Yu Shuai Wang Huimin Li Xinyiran Du Xiaodong He |
| author_facet | Tianshu Liu Hai Yu Shuai Wang Huimin Li Xinyiran Du Xiaodong He |
| author_sort | Tianshu Liu |
| collection | DOAJ |
| description | Abstract The use of non-drug intervention for calcium deficiency has attracted attention in recent years. Although calcium carbonate is the preferred raw material for calcium supplementation, there are few reports on the mechanism of the combined action of chondroitin sulfate and calcium to alleviate osteoporosis from the perspective of gut microbiota and metabolomics. In this study, a rat model of osteoporosis was established by feeding a low-calcium diet. The intestinal microbiota abundance, fecal and plasma metabolite expression levels of rats fed a basal diet, a low-calcium diet, a low-calcium diet plus calcium carbonate, and a low-calcium diet plus chondroitin sulfate were compared. The results showed that compared with the low calcium group, the calcium content and bone mineral density of femur were significantly increased in the calcium carbonate and chondroitin sulfate groups. 16 S rRNA sequencing and metabolomics analysis showed that chondroitin sulfate intervention could reduce short-chain fatty acid synthesis of intestinal flora, slow down inflammatory response, inhibit osteoclast differentiation, promote calcium absorption and antioxidant mechanism, and alleviate osteoporosis in low-calcium feeding rats. Correlation analysis showed that the selected intestinal flora was significantly correlated with metabolites enriched in feces and plasma. This study provides scientific evidence of the potential impact of chondroitin sulfate as a dietary supplement for patients with osteoporosis. |
| first_indexed | 2024-04-10T15:46:32Z |
| format | Article |
| id | doaj.art-8d5bac72057b479da347faefbfae2c74 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-04-10T15:46:32Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-8d5bac72057b479da347faefbfae2c742023-02-12T12:06:08ZengBMCNutrition & Metabolism1743-70752023-02-0120111810.1186/s12986-023-00726-3Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolismTianshu Liu0Hai Yu1Shuai Wang2Huimin Li3Xinyiran Du4Xiaodong He5Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong UniversityDivision of Cancer RNA Research, National Cancer Center Research InstituteInstitute of Toxicology, School of Public Health, Cheeloo College of Medicine, Shandong UniversityDepartment of Physical and Chemical Inspection, School of Public Health, Cheeloo College of Medicine, Shandong UniversityCollege of Stomatology, Jining Medical UniversityDepartment of Physical and Chemical Inspection, School of Public Health, Cheeloo College of Medicine, Shandong UniversityAbstract The use of non-drug intervention for calcium deficiency has attracted attention in recent years. Although calcium carbonate is the preferred raw material for calcium supplementation, there are few reports on the mechanism of the combined action of chondroitin sulfate and calcium to alleviate osteoporosis from the perspective of gut microbiota and metabolomics. In this study, a rat model of osteoporosis was established by feeding a low-calcium diet. The intestinal microbiota abundance, fecal and plasma metabolite expression levels of rats fed a basal diet, a low-calcium diet, a low-calcium diet plus calcium carbonate, and a low-calcium diet plus chondroitin sulfate were compared. The results showed that compared with the low calcium group, the calcium content and bone mineral density of femur were significantly increased in the calcium carbonate and chondroitin sulfate groups. 16 S rRNA sequencing and metabolomics analysis showed that chondroitin sulfate intervention could reduce short-chain fatty acid synthesis of intestinal flora, slow down inflammatory response, inhibit osteoclast differentiation, promote calcium absorption and antioxidant mechanism, and alleviate osteoporosis in low-calcium feeding rats. Correlation analysis showed that the selected intestinal flora was significantly correlated with metabolites enriched in feces and plasma. This study provides scientific evidence of the potential impact of chondroitin sulfate as a dietary supplement for patients with osteoporosis.https://doi.org/10.1186/s12986-023-00726-3OsteoporosisChondroitin sulfateIntestinal floraMetabolomicsLipid metabolism |
| spellingShingle | Tianshu Liu Hai Yu Shuai Wang Huimin Li Xinyiran Du Xiaodong He Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism Nutrition & Metabolism Osteoporosis Chondroitin sulfate Intestinal flora Metabolomics Lipid metabolism |
| title | Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism |
| title_full | Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism |
| title_fullStr | Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism |
| title_full_unstemmed | Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism |
| title_short | Chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism |
| title_sort | chondroitin sulfate alleviates osteoporosis caused by calcium deficiency by regulating lipid metabolism |
| topic | Osteoporosis Chondroitin sulfate Intestinal flora Metabolomics Lipid metabolism |
| url | https://doi.org/10.1186/s12986-023-00726-3 |
| work_keys_str_mv | AT tianshuliu chondroitinsulfatealleviatesosteoporosiscausedbycalciumdeficiencybyregulatinglipidmetabolism AT haiyu chondroitinsulfatealleviatesosteoporosiscausedbycalciumdeficiencybyregulatinglipidmetabolism AT shuaiwang chondroitinsulfatealleviatesosteoporosiscausedbycalciumdeficiencybyregulatinglipidmetabolism AT huiminli chondroitinsulfatealleviatesosteoporosiscausedbycalciumdeficiencybyregulatinglipidmetabolism AT xinyirandu chondroitinsulfatealleviatesosteoporosiscausedbycalciumdeficiencybyregulatinglipidmetabolism AT xiaodonghe chondroitinsulfatealleviatesosteoporosiscausedbycalciumdeficiencybyregulatinglipidmetabolism |