Genetic identification of ophichthid fishes through DNA barcoding
Ophichthidae is the most ecologically diverse family of the order Anguilliformes; however, high morphological similarity and a long larval stage make precise identification difficult. The aim of the present study was to examine the potential for barcoding of ophichthid fishes. A total of 81 mitochon...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-04-01
|
| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2020.1756940 |
| _version_ | 1797639226575028224 |
|---|---|
| author | Bingpeng Xing Xiaoyin Chen Zhilan Zhang Rouxin Sun Peng Xiang Mao Lin Chunguang Wang |
| author_facet | Bingpeng Xing Xiaoyin Chen Zhilan Zhang Rouxin Sun Peng Xiang Mao Lin Chunguang Wang |
| author_sort | Bingpeng Xing |
| collection | DOAJ |
| description | Ophichthidae is the most ecologically diverse family of the order Anguilliformes; however, high morphological similarity and a long larval stage make precise identification difficult. The aim of the present study was to examine the potential for barcoding of ophichthid fishes. A total of 81 mitochondrial cytochrome C oxidase subunit I (COI) barcode sequences were obtained from 23 species and 12 genera of ophichthid fishes collected in the Taiwan Strait. The average Kimura two-parameter distances within species, genera, and families were 0.48%, 18.25%, and 18.79%, respectively; thus, the mean interspecific distance was 31-fold higher than the mean intraspecific distance, which demonstrates good efficiency in DNA barcoding. The sequence divergence among species also varied substantially from 4.60% to 25.90%. This evaluation demonstrates the high efficiency of DNA barcoding for species identification to facilitate research on the diversity and conservation of ophichthid fishes. |
| first_indexed | 2024-03-11T13:14:50Z |
| format | Article |
| id | doaj.art-8d5be2e0362c4ad0b5f9ec5d082b66b8 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:14:50Z |
| publishDate | 2020-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-8d5be2e0362c4ad0b5f9ec5d082b66b82023-11-03T13:50:21ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592020-04-01521970197410.1080/23802359.2020.17569401756940Genetic identification of ophichthid fishes through DNA barcodingBingpeng Xing0Xiaoyin Chen1Zhilan Zhang2Rouxin Sun3Peng Xiang4Mao Lin5Chunguang Wang6Third Institute of Oceanography Ministry of Natural ResourcesThird Institute of Oceanography Ministry of Natural ResourcesThird Institute of Oceanography Ministry of Natural ResourcesThird Institute of Oceanography Ministry of Natural ResourcesThird Institute of Oceanography Ministry of Natural ResourcesThird Institute of Oceanography Ministry of Natural ResourcesThird Institute of Oceanography Ministry of Natural ResourcesOphichthidae is the most ecologically diverse family of the order Anguilliformes; however, high morphological similarity and a long larval stage make precise identification difficult. The aim of the present study was to examine the potential for barcoding of ophichthid fishes. A total of 81 mitochondrial cytochrome C oxidase subunit I (COI) barcode sequences were obtained from 23 species and 12 genera of ophichthid fishes collected in the Taiwan Strait. The average Kimura two-parameter distances within species, genera, and families were 0.48%, 18.25%, and 18.79%, respectively; thus, the mean interspecific distance was 31-fold higher than the mean intraspecific distance, which demonstrates good efficiency in DNA barcoding. The sequence divergence among species also varied substantially from 4.60% to 25.90%. This evaluation demonstrates the high efficiency of DNA barcoding for species identification to facilitate research on the diversity and conservation of ophichthid fishes.http://dx.doi.org/10.1080/23802359.2020.1756940ophichthididentificationbiodiversitydna barcodingcoi |
| spellingShingle | Bingpeng Xing Xiaoyin Chen Zhilan Zhang Rouxin Sun Peng Xiang Mao Lin Chunguang Wang Genetic identification of ophichthid fishes through DNA barcoding Mitochondrial DNA. Part B. Resources ophichthid identification biodiversity dna barcoding coi |
| title | Genetic identification of ophichthid fishes through DNA barcoding |
| title_full | Genetic identification of ophichthid fishes through DNA barcoding |
| title_fullStr | Genetic identification of ophichthid fishes through DNA barcoding |
| title_full_unstemmed | Genetic identification of ophichthid fishes through DNA barcoding |
| title_short | Genetic identification of ophichthid fishes through DNA barcoding |
| title_sort | genetic identification of ophichthid fishes through dna barcoding |
| topic | ophichthid identification biodiversity dna barcoding coi |
| url | http://dx.doi.org/10.1080/23802359.2020.1756940 |
| work_keys_str_mv | AT bingpengxing geneticidentificationofophichthidfishesthroughdnabarcoding AT xiaoyinchen geneticidentificationofophichthidfishesthroughdnabarcoding AT zhilanzhang geneticidentificationofophichthidfishesthroughdnabarcoding AT rouxinsun geneticidentificationofophichthidfishesthroughdnabarcoding AT pengxiang geneticidentificationofophichthidfishesthroughdnabarcoding AT maolin geneticidentificationofophichthidfishesthroughdnabarcoding AT chunguangwang geneticidentificationofophichthidfishesthroughdnabarcoding |