Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum)
Superoxide dismutases (SODs) are a family of key antioxidant enzymes that play a crucial role in plant growth and development. Previously, this gene family has been investigated in Arabidopsis and rice. In the present study, a genome-wide analysis of the SOD gene family in wheat were performed. Twen...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-11-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8062.pdf |
| _version_ | 1797419303595671552 |
|---|---|
| author | Wenqiang Jiang Lei Yang Yiqin He Haotian Zhang Wei Li Huaigu Chen Dongfang Ma Junliang Yin |
| author_facet | Wenqiang Jiang Lei Yang Yiqin He Haotian Zhang Wei Li Huaigu Chen Dongfang Ma Junliang Yin |
| author_sort | Wenqiang Jiang |
| collection | DOAJ |
| description | Superoxide dismutases (SODs) are a family of key antioxidant enzymes that play a crucial role in plant growth and development. Previously, this gene family has been investigated in Arabidopsis and rice. In the present study, a genome-wide analysis of the SOD gene family in wheat were performed. Twenty-six SOD genes were identified from the whole genome of wheat, including 17 Cu/Zn-SODs, six Fe-SODs, and three Mn-SODs. The chromosomal location mapping analysis indicated that these three types of SOD genes were only distributed on 2, 4, and 7 chromosomes, respectively. Phylogenetic analyses of wheat SODs and several other species revealed that these SOD proteins can be assigned to two major categories. SOD1 mainly comprises of Cu/Zn-SODs, and SOD2 mainly comprises of Fe-SODs and Mn-SODs. Gene structure and motif analyses indicated that most of the SOD genes showed a relatively conserved exon/intron arrangement and motif composition. Analyses of transcriptional data indicated that most of the wheat SOD genes were expressed in almost all of the examined tissues and had important functions in abiotic stress resistance. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to reveal the regulating roles of wheat SOD gene family in response to NaCl, mannitol, and polyethylene glycol stresses. qRT-PCR showed that eight randomly selected genes with relatively high expression levels responded to all three stresses based on released transcriptome data. However, their degree of response and response patterns were different. Interestingly, among these genes, TaSOD1.7, TaSOD1.9, TaSOD2.1, and TaSOD2.3 feature research value owing to their remarkable expression-fold change in leaves or roots under different stresses. Overall, our results provide a basis of further functional research on the SOD gene family in wheat and facilitate their potential use for applications in the genetic improvement on wheat in drought and salt stress environments. |
| first_indexed | 2024-03-09T06:45:26Z |
| format | Article |
| id | doaj.art-8d5ddb093f034e8a9d1a1c1388cf3d47 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:45:26Z |
| publishDate | 2019-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-8d5ddb093f034e8a9d1a1c1388cf3d472023-12-03T10:37:07ZengPeerJ Inc.PeerJ2167-83592019-11-017e806210.7717/peerj.8062Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum)Wenqiang Jiang0Lei Yang1Yiqin He2Haotian Zhang3Wei Li4Huaigu Chen5Dongfang Ma6Junliang Yin7Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou, Hubei, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou, Hubei, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou, Hubei, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou, Hubei, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou, Hubei, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou, Hubei, ChinaSuperoxide dismutases (SODs) are a family of key antioxidant enzymes that play a crucial role in plant growth and development. Previously, this gene family has been investigated in Arabidopsis and rice. In the present study, a genome-wide analysis of the SOD gene family in wheat were performed. Twenty-six SOD genes were identified from the whole genome of wheat, including 17 Cu/Zn-SODs, six Fe-SODs, and three Mn-SODs. The chromosomal location mapping analysis indicated that these three types of SOD genes were only distributed on 2, 4, and 7 chromosomes, respectively. Phylogenetic analyses of wheat SODs and several other species revealed that these SOD proteins can be assigned to two major categories. SOD1 mainly comprises of Cu/Zn-SODs, and SOD2 mainly comprises of Fe-SODs and Mn-SODs. Gene structure and motif analyses indicated that most of the SOD genes showed a relatively conserved exon/intron arrangement and motif composition. Analyses of transcriptional data indicated that most of the wheat SOD genes were expressed in almost all of the examined tissues and had important functions in abiotic stress resistance. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to reveal the regulating roles of wheat SOD gene family in response to NaCl, mannitol, and polyethylene glycol stresses. qRT-PCR showed that eight randomly selected genes with relatively high expression levels responded to all three stresses based on released transcriptome data. However, their degree of response and response patterns were different. Interestingly, among these genes, TaSOD1.7, TaSOD1.9, TaSOD2.1, and TaSOD2.3 feature research value owing to their remarkable expression-fold change in leaves or roots under different stresses. Overall, our results provide a basis of further functional research on the SOD gene family in wheat and facilitate their potential use for applications in the genetic improvement on wheat in drought and salt stress environments.https://peerj.com/articles/8062.pdfSODGene structureProtein characterizationAbiotic stressExpression profiles |
| spellingShingle | Wenqiang Jiang Lei Yang Yiqin He Haotian Zhang Wei Li Huaigu Chen Dongfang Ma Junliang Yin Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) PeerJ SOD Gene structure Protein characterization Abiotic stress Expression profiles |
| title | Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) |
| title_full | Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) |
| title_fullStr | Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) |
| title_full_unstemmed | Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) |
| title_short | Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) |
| title_sort | genome wide identification and transcriptional expression analysis of superoxide dismutase sod family in wheat triticum aestivum |
| topic | SOD Gene structure Protein characterization Abiotic stress Expression profiles |
| url | https://peerj.com/articles/8062.pdf |
| work_keys_str_mv | AT wenqiangjiang genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT leiyang genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT yiqinhe genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT haotianzhang genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT weili genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT huaiguchen genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT dongfangma genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum AT junliangyin genomewideidentificationandtranscriptionalexpressionanalysisofsuperoxidedismutasesodfamilyinwheattriticumaestivum |