Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro
Porcine Epidemic Diarrhea Virus (PEDV) is a highly contagious and pathogenic virus that causes symptoms such as diarrhea, vomiting, weight loss, and even death in piglets. Due to its high transmission rate, PEDV has resulted in significant global losses. Although some vaccines have been developed an...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023086760 |
| _version_ | 1797429858880454656 |
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| author | Zi-Xin Huang Shu-Ting Zhou Jing Wang Zhi-Biao Yang Zhe Wang |
| author_facet | Zi-Xin Huang Shu-Ting Zhou Jing Wang Zhi-Biao Yang Zhe Wang |
| author_sort | Zi-Xin Huang |
| collection | DOAJ |
| description | Porcine Epidemic Diarrhea Virus (PEDV) is a highly contagious and pathogenic virus that causes symptoms such as diarrhea, vomiting, weight loss, and even death in piglets. Due to its high transmission rate, PEDV has resulted in significant global losses. Although some vaccines have been developed and utilized to prevent PEDV, their effectiveness is limited due to the virus's mutations. Therefore, it is imperative to investigate new strategies to combat PEDV. Remdesivir, a classic antiviral drug for coronaviruses, has been proven in our experiment to effectively suppress PEDV replication in Vero and LLC-PK1 cells. Additionally, the cell experiment demonstrated its direct inhibition of PEDV RNA-dependent RNA polymerase (RdRp) enzyme activity. Molecular docking simulations were employed to predict the binding site of remdesivir and PEDV RdRp. Moreover, we observed that remdesivir does not impact the production of inflammatory factors and exhibits antagonistic effects with exogenous nucleosides. Furthermore, we conducted RNA-Seq analysis to investigate the global changes in transcriptome of infected cells treated with remdesivir. Overall, our findings indicate that remdesivir holds promise as a potential candidate for the treatment of PEDV infection. |
| first_indexed | 2024-03-09T09:19:52Z |
| format | Article |
| id | doaj.art-8d5fb783c1b74dfe83a80065d43bed09 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:19:52Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8d5fb783c1b74dfe83a80065d43bed092023-12-02T07:02:27ZengElsevierHeliyon2405-84402023-11-01911e21468Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitroZi-Xin Huang0Shu-Ting Zhou1Jing Wang2Zhi-Biao Yang3Zhe Wang4Shanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yang ling, Xianyang 712100, ChinaShanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding author. Shanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Shanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding author. Shanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Porcine Epidemic Diarrhea Virus (PEDV) is a highly contagious and pathogenic virus that causes symptoms such as diarrhea, vomiting, weight loss, and even death in piglets. Due to its high transmission rate, PEDV has resulted in significant global losses. Although some vaccines have been developed and utilized to prevent PEDV, their effectiveness is limited due to the virus's mutations. Therefore, it is imperative to investigate new strategies to combat PEDV. Remdesivir, a classic antiviral drug for coronaviruses, has been proven in our experiment to effectively suppress PEDV replication in Vero and LLC-PK1 cells. Additionally, the cell experiment demonstrated its direct inhibition of PEDV RNA-dependent RNA polymerase (RdRp) enzyme activity. Molecular docking simulations were employed to predict the binding site of remdesivir and PEDV RdRp. Moreover, we observed that remdesivir does not impact the production of inflammatory factors and exhibits antagonistic effects with exogenous nucleosides. Furthermore, we conducted RNA-Seq analysis to investigate the global changes in transcriptome of infected cells treated with remdesivir. Overall, our findings indicate that remdesivir holds promise as a potential candidate for the treatment of PEDV infection.http://www.sciencedirect.com/science/article/pii/S2405844023086760Porcine epidemic diarrhea virusremdesivirReplication inhibitionRdRpRNA-Seq |
| spellingShingle | Zi-Xin Huang Shu-Ting Zhou Jing Wang Zhi-Biao Yang Zhe Wang Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro Heliyon Porcine epidemic diarrhea virus remdesivir Replication inhibition RdRp RNA-Seq |
| title | Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro |
| title_full | Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro |
| title_fullStr | Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro |
| title_full_unstemmed | Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro |
| title_short | Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro |
| title_sort | remdesivir inhibits porcine epidemic diarrhea virus infection in vitro |
| topic | Porcine epidemic diarrhea virus remdesivir Replication inhibition RdRp RNA-Seq |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023086760 |
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