Screening and biological characteristics of excellent lactic acid bacteria from Kefir

Four excellent lactic acid bacteria were screened from Kefir. After morphological observation, physical and chemical detection, and 16SrDNA sequence comparison, three strains were preliminarily identified as Lactobacillus Kefir, and one strain was Lactobacillus acidophilus. Two strains were selected...

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Main Authors: Guo Zhihua, Batbatan Christopher
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2023/04/bioconf_icbb2023_01018.pdf
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author Guo Zhihua
Batbatan Christopher
author_facet Guo Zhihua
Batbatan Christopher
author_sort Guo Zhihua
collection DOAJ
description Four excellent lactic acid bacteria were screened from Kefir. After morphological observation, physical and chemical detection, and 16SrDNA sequence comparison, three strains were preliminarily identified as Lactobacillus Kefir, and one strain was Lactobacillus acidophilus. Two strains were selected to study their biological characteristics. The results showed that the lactic acid bacteria isolated from Kefir had good probiotic properties, with a survival rate of more than 42% after artificial gastric juice digestion for 3 hours and more than 46% after artificial intestinal juice digestion for 6 hours; It has inhibitory effects on intestinal pathogenic bacteria such as Staphylococcus aureus and Escherichia coli; Does not produce hemolysis; There are different levels of resistance to antibiotics.
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spelling doaj.art-8d6008d9995e4bd38e7a06d7ca16b6e82023-05-11T09:08:11ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01590101810.1051/bioconf/20235901018bioconf_icbb2023_01018Screening and biological characteristics of excellent lactic acid bacteria from KefirGuo Zhihua0Batbatan Christopher1Department of biology, College of Arts and Sciences, Central Mindanao UniversityDepartment of biology, College of Arts and Sciences, Central Mindanao UniversityFour excellent lactic acid bacteria were screened from Kefir. After morphological observation, physical and chemical detection, and 16SrDNA sequence comparison, three strains were preliminarily identified as Lactobacillus Kefir, and one strain was Lactobacillus acidophilus. Two strains were selected to study their biological characteristics. The results showed that the lactic acid bacteria isolated from Kefir had good probiotic properties, with a survival rate of more than 42% after artificial gastric juice digestion for 3 hours and more than 46% after artificial intestinal juice digestion for 6 hours; It has inhibitory effects on intestinal pathogenic bacteria such as Staphylococcus aureus and Escherichia coli; Does not produce hemolysis; There are different levels of resistance to antibiotics.https://www.bio-conferences.org/articles/bioconf/pdf/2023/04/bioconf_icbb2023_01018.pdf
spellingShingle Guo Zhihua
Batbatan Christopher
Screening and biological characteristics of excellent lactic acid bacteria from Kefir
BIO Web of Conferences
title Screening and biological characteristics of excellent lactic acid bacteria from Kefir
title_full Screening and biological characteristics of excellent lactic acid bacteria from Kefir
title_fullStr Screening and biological characteristics of excellent lactic acid bacteria from Kefir
title_full_unstemmed Screening and biological characteristics of excellent lactic acid bacteria from Kefir
title_short Screening and biological characteristics of excellent lactic acid bacteria from Kefir
title_sort screening and biological characteristics of excellent lactic acid bacteria from kefir
url https://www.bio-conferences.org/articles/bioconf/pdf/2023/04/bioconf_icbb2023_01018.pdf
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