UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum
In this study, a total of 66 UDP-glucose pyrophosphorylase (UGP) (EC 2.7.7.9) genes were identified from the genomes of four cotton species, which are the members of Pfam glycosyltransferase family (PF01702) and catalyze the reaction between glucose-1-phosphate and UTP to produce UDPG. The analysis...
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PeerJ Inc.
2022-05-01
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| Online Access: | https://peerj.com/articles/13460.pdf |
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| author | Zhongyang Xu Jiasen He Muhammad Tehseen Azhar Zhen Zhang Senmiao Fan Xiao Jiang Tingting Jia Haihong Shang Youlu Yuan |
| author_facet | Zhongyang Xu Jiasen He Muhammad Tehseen Azhar Zhen Zhang Senmiao Fan Xiao Jiang Tingting Jia Haihong Shang Youlu Yuan |
| author_sort | Zhongyang Xu |
| collection | DOAJ |
| description | In this study, a total of 66 UDP-glucose pyrophosphorylase (UGP) (EC 2.7.7.9) genes were identified from the genomes of four cotton species, which are the members of Pfam glycosyltransferase family (PF01702) and catalyze the reaction between glucose-1-phosphate and UTP to produce UDPG. The analysis of evolutionary relationship, gene structure, and expression provides the basis for studies on function of UGP genes in cotton. The evolutionary tree and gene structure analysis revealed that the UGP gene family is evolutionarily conserved. Collinearity and Ka/Ks analysis indicated that amplification of UGP genes is due to repetitive crosstalk generating between new family genes, while being under strong selection pressure. The analysis of cis-acting elements exhibited that UGP genes play important role in cotton growth, development, abiotic and hormonal stresses. Six UGP genes that were highly expressed in cotton fiber at 15 DPA were screened by transcriptome data and qRT-PCR analysis. The addition of low concentrations of IAA and GA3 to ovule cultures revealed that energy efficiency promoted the development of ovules and fiber clusters, and qRT-PCR showed that expression of these six UGP genes was differentially increased. These results suggest that the UGP gene may play an important role in fiber development, and provides the opportunity to plant researchers to explore the mechanisms involve in fiber development in cotton. |
| first_indexed | 2024-03-09T08:08:45Z |
| format | Article |
| id | doaj.art-8d60381c22eb491eb0a00f06479bd00b |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:08:45Z |
| publishDate | 2022-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-8d60381c22eb491eb0a00f06479bd00b2023-12-02T23:35:56ZengPeerJ Inc.PeerJ2167-83592022-05-0110e1346010.7717/peerj.13460UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutumZhongyang Xu0Jiasen He1Muhammad Tehseen Azhar2Zhen Zhang3Senmiao Fan4Xiao Jiang5Tingting Jia6Haihong Shang7Youlu Yuan8Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Plant Breeding and Genetics, University of Agriculture, Faisalabad, PakistanState Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministryof Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministryof Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministryof Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministryof Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, ChinaState Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministryof Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaIn this study, a total of 66 UDP-glucose pyrophosphorylase (UGP) (EC 2.7.7.9) genes were identified from the genomes of four cotton species, which are the members of Pfam glycosyltransferase family (PF01702) and catalyze the reaction between glucose-1-phosphate and UTP to produce UDPG. The analysis of evolutionary relationship, gene structure, and expression provides the basis for studies on function of UGP genes in cotton. The evolutionary tree and gene structure analysis revealed that the UGP gene family is evolutionarily conserved. Collinearity and Ka/Ks analysis indicated that amplification of UGP genes is due to repetitive crosstalk generating between new family genes, while being under strong selection pressure. The analysis of cis-acting elements exhibited that UGP genes play important role in cotton growth, development, abiotic and hormonal stresses. Six UGP genes that were highly expressed in cotton fiber at 15 DPA were screened by transcriptome data and qRT-PCR analysis. The addition of low concentrations of IAA and GA3 to ovule cultures revealed that energy efficiency promoted the development of ovules and fiber clusters, and qRT-PCR showed that expression of these six UGP genes was differentially increased. These results suggest that the UGP gene may play an important role in fiber development, and provides the opportunity to plant researchers to explore the mechanisms involve in fiber development in cotton.https://peerj.com/articles/13460.pdfCottonFiber developmentUDP-glucose pyrophosphorylase |
| spellingShingle | Zhongyang Xu Jiasen He Muhammad Tehseen Azhar Zhen Zhang Senmiao Fan Xiao Jiang Tingting Jia Haihong Shang Youlu Yuan UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum PeerJ Cotton Fiber development UDP-glucose pyrophosphorylase |
| title | UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum |
| title_full | UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum |
| title_fullStr | UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum |
| title_full_unstemmed | UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum |
| title_short | UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum |
| title_sort | udp glucose pyrophosphorylase genome wide identification expression and functional analyses in gossypium hirsutum |
| topic | Cotton Fiber development UDP-glucose pyrophosphorylase |
| url | https://peerj.com/articles/13460.pdf |
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