Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss
Like most of the medicinal plants Soymida febrifuga (Meliaceae) possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protoco...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2014-03-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9202 |
| _version_ | 1828522823708049408 |
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| author | Kishore Kumar CHIRUVELLA Arifullah MOHAMMED Rama Gopal GHANTA |
| author_facet | Kishore Kumar CHIRUVELLA Arifullah MOHAMMED Rama Gopal GHANTA |
| author_sort | Kishore Kumar CHIRUVELLA |
| collection | DOAJ |
| description | Like most of the medicinal plants Soymida febrifuga (Meliaceae) possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protocols for in vitro mass propagation has been established from field grown and aseptic seedlings explants. Strikingly, we observed aberrant structures such as vitrified shoots, faciated shoots, albino shoots as well shoot necrosis during its micropropagation. These phenotypic maladies were observed during organogenesis and rooting. Compared to other abnormalities, shoot necrosis nonetheless was frequent and pronounced leading to plant death. Shoots when subjected to rooting also displayed necrosis which was controlled by transferring to MS medium containing various concentrations and combinations of calcium levels, activated charcoal, glucose, fructose and auxins. Microshoots initiated roots on half strength MS medium with IBA and IAA individually or in combination within two weeks. MS half strength solid medium supplemented with CAN (556 mg l–1), CAP (1.0 mg l–1), IAA (2.0 mg l–1) and IBA (2.0 mg l–1) in combination was found to be more efficient in showing high frequency (95%) of root regeneration. Rooted plantlets were successfully hardened and 70-85% of regenerated plants were successfully acclimatized to natural environment. In vitro derived plantlets were morphologically similar to in vivo plants. |
| first_indexed | 2024-12-11T20:16:14Z |
| format | Article |
| id | doaj.art-8d609a76f63145b2b54fd974674caa6e |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-11T20:16:14Z |
| publishDate | 2014-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-8d609a76f63145b2b54fd974674caa6e2022-12-22T00:52:11ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642014-03-01619910410.15835/nsb6192028014Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. JussKishore Kumar CHIRUVELLA0Arifullah MOHAMMED1Rama Gopal GHANTA2Postdoctoral fellow, stockholm universityDivision of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600, Jeli, KelantanDivision of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra PradeshLike most of the medicinal plants Soymida febrifuga (Meliaceae) possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protocols for in vitro mass propagation has been established from field grown and aseptic seedlings explants. Strikingly, we observed aberrant structures such as vitrified shoots, faciated shoots, albino shoots as well shoot necrosis during its micropropagation. These phenotypic maladies were observed during organogenesis and rooting. Compared to other abnormalities, shoot necrosis nonetheless was frequent and pronounced leading to plant death. Shoots when subjected to rooting also displayed necrosis which was controlled by transferring to MS medium containing various concentrations and combinations of calcium levels, activated charcoal, glucose, fructose and auxins. Microshoots initiated roots on half strength MS medium with IBA and IAA individually or in combination within two weeks. MS half strength solid medium supplemented with CAN (556 mg l–1), CAP (1.0 mg l–1), IAA (2.0 mg l–1) and IBA (2.0 mg l–1) in combination was found to be more efficient in showing high frequency (95%) of root regeneration. Rooted plantlets were successfully hardened and 70-85% of regenerated plants were successfully acclimatized to natural environment. In vitro derived plantlets were morphologically similar to in vivo plants.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9202 |
| spellingShingle | Kishore Kumar CHIRUVELLA Arifullah MOHAMMED Rama Gopal GHANTA Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss Notulae Scientia Biologicae |
| title | Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss |
| title_full | Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss |
| title_fullStr | Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss |
| title_full_unstemmed | Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss |
| title_short | Phenotypic aberrations during micropropagation of <i>Soymida febrifuga</i> (Roxb.) Adr. Juss |
| title_sort | phenotypic aberrations during micropropagation of lt i gt soymida febrifuga lt i gt roxb adr juss |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9202 |
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