Untargeted stable isotope-resolved metabolomics to assess the effect of PI3Kβ inhibition on metabolic pathway activities in a PTEN null breast cancer cell line

Untargeted stable isotope-resolved metabolomics to assess the effect of PI3Kβ inhibition on metabolic pathway activities in a PTEN null breast cancer cell line

The combination of high-resolution LC-MS untargeted metabolomics with stable isotope-resolved tracing is a promising approach for the global exploration of metabolic pathway activities. In our established workflow we combine targeted isotopologue feature extraction with the non-targeted X13CMS routi...

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Bibliographic Details
Main Authors: Marcel Lackner, Sylvia K. Neef, Stefan Winter, Sandra Beer-Hammer, Bernd Nürnberg, Matthias Schwab, Ute Hofmann, Mathias Haag
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Molecular Biosciences
Subjects:
non-targeted analysis
13C labeling
LC-QTOF-MS
glycolysis
TCA cycle
PI3K
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.1004602/full

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