Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study
Diffuse malignant peritoneal mesothelioma (DMPM) is a rare malignant neoplasm with a poor survival. Although some advances in knowledge have been obtained for the pleural form, much less is known about DMPM. Advantages in terms of prognosis are still limited and strong efforts need to be made. The a...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/6/386 |
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| author | Federica Pezzuto Gabriella Serio Francesco Fortarezza Anna Scattone Concetta Caporusso Alessandra Punzi Domenica Cavone Antonio Pennella Andrea Marzullo Luigi Vimercati |
| author_facet | Federica Pezzuto Gabriella Serio Francesco Fortarezza Anna Scattone Concetta Caporusso Alessandra Punzi Domenica Cavone Antonio Pennella Andrea Marzullo Luigi Vimercati |
| author_sort | Federica Pezzuto |
| collection | DOAJ |
| description | Diffuse malignant peritoneal mesothelioma (DMPM) is a rare malignant neoplasm with a poor survival. Although some advances in knowledge have been obtained for the pleural form, much less is known about DMPM. Advantages in terms of prognosis are still limited and strong efforts need to be made. The aim of our study was to correlate several histological and molecular factors with survival in a large cohort of 45 DMPMs. We evaluated histotype, nuclear grade, mitotic count, necrosis, inflammation, desmoplastic reaction, Ki67 percentage, WT-1 expression, p16 protein by immunohistochemistry and CDKN2A deletion by FISH. Our results showed that epithelioid histotype, nuclear grade 2, mitotic count ≤5 x mm<sup>2</sup>, absence of desmoplasia and p16/CDKN2A deletion, low Ki67 value, and high WT-1 expression were correlated with the most prolonged survival (<i>p</i> = 0.0001). Moreover, p16 loss in immunohistochemistry reflected CDKN2A deletion detected with FISH, and both were correlated with the worst survival (<i>p</i> = 0.0001). At multivariate analysis, Ki67 value, WT-1 expression and p16/CDKN2A deletion emerged as independent prognostic factors (<i>p</i> = 0.01, <i>p</i> = 0.0001 and <i>p</i> = 0.01, respectively). These parameters are easy to analyse at the time of DMPM diagnosis and may support better patient stratification, prediction of treatment effectiveness and therapeutic optimization. |
| first_indexed | 2024-03-10T19:17:05Z |
| format | Article |
| id | doaj.art-8d6247ea91b048c49a7e7cc6ce391717 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T19:17:05Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-8d6247ea91b048c49a7e7cc6ce3917172023-11-20T03:18:01ZengMDPI AGDiagnostics2075-44182020-06-0110638610.3390/diagnostics10060386Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort StudyFederica Pezzuto0Gabriella Serio1Francesco Fortarezza2Anna Scattone3Concetta Caporusso4Alessandra Punzi5Domenica Cavone6Antonio Pennella7Andrea Marzullo8Luigi Vimercati9Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, ItalyPathology Division, Department of Emergency and Organ Transplantation, University of Bari, 70124 Bari, ItalyDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, ItalyPathology Division, IRCCS National Cancer Institute “Giovanni Paolo II”, 70124 Bari, ItalyPathology Division, Department of Emergency and Organ Transplantation, University of Bari, 70124 Bari, ItalyPathology Division, Department of Emergency and Organ Transplantation, University of Bari, 70124 Bari, ItalyOccupational Health Division, Department of Interdisciplinary Medicine, University of Bari, 70124 Bari, ItalyPathology Division, Department of Surgery, University of Foggia, 71122 Foggia, ItalyPathology Division, Department of Emergency and Organ Transplantation, University of Bari, 70124 Bari, ItalyOccupational Health Division, Department of Interdisciplinary Medicine, University of Bari, 70124 Bari, ItalyDiffuse malignant peritoneal mesothelioma (DMPM) is a rare malignant neoplasm with a poor survival. Although some advances in knowledge have been obtained for the pleural form, much less is known about DMPM. Advantages in terms of prognosis are still limited and strong efforts need to be made. The aim of our study was to correlate several histological and molecular factors with survival in a large cohort of 45 DMPMs. We evaluated histotype, nuclear grade, mitotic count, necrosis, inflammation, desmoplastic reaction, Ki67 percentage, WT-1 expression, p16 protein by immunohistochemistry and CDKN2A deletion by FISH. Our results showed that epithelioid histotype, nuclear grade 2, mitotic count ≤5 x mm<sup>2</sup>, absence of desmoplasia and p16/CDKN2A deletion, low Ki67 value, and high WT-1 expression were correlated with the most prolonged survival (<i>p</i> = 0.0001). Moreover, p16 loss in immunohistochemistry reflected CDKN2A deletion detected with FISH, and both were correlated with the worst survival (<i>p</i> = 0.0001). At multivariate analysis, Ki67 value, WT-1 expression and p16/CDKN2A deletion emerged as independent prognostic factors (<i>p</i> = 0.01, <i>p</i> = 0.0001 and <i>p</i> = 0.01, respectively). These parameters are easy to analyse at the time of DMPM diagnosis and may support better patient stratification, prediction of treatment effectiveness and therapeutic optimization.https://www.mdpi.com/2075-4418/10/6/386diffuse malignant peritoneal mesotheliomaprognostic factorsKi67WT-1p16CDKN2A |
| spellingShingle | Federica Pezzuto Gabriella Serio Francesco Fortarezza Anna Scattone Concetta Caporusso Alessandra Punzi Domenica Cavone Antonio Pennella Andrea Marzullo Luigi Vimercati Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study Diagnostics diffuse malignant peritoneal mesothelioma prognostic factors Ki67 WT-1 p16 CDKN2A |
| title | Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study |
| title_full | Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study |
| title_fullStr | Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study |
| title_full_unstemmed | Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study |
| title_short | Prognostic Value of Ki67 Percentage, WT-1 Expression and p16/CDKN2A Deletion in Diffuse Malignant Peritoneal Mesothelioma: A Single-Centre Cohort Study |
| title_sort | prognostic value of ki67 percentage wt 1 expression and p16 cdkn2a deletion in diffuse malignant peritoneal mesothelioma a single centre cohort study |
| topic | diffuse malignant peritoneal mesothelioma prognostic factors Ki67 WT-1 p16 CDKN2A |
| url | https://www.mdpi.com/2075-4418/10/6/386 |
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