Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study
The aim of this study is to investigate the effect of doxycycline collagen sponge on bisphosphonate-related osteonecrosis of the jaw (BRONJ) and the level of serum biomarkers as an indicator of osteonecrosis. Twenty-four rats were divided into four groups. Animals in the control group were injected...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-07-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1174078 |
| _version_ | 1818499870847139840 |
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| author | Gonca Duygu Çapar Melike Sapmaz-Metin Esma Kütan Ceyda Ozcakir Tomruk Gül Merve Yalcin Nilay Er Gulnur Kizilay Ozfidan |
| author_facet | Gonca Duygu Çapar Melike Sapmaz-Metin Esma Kütan Ceyda Ozcakir Tomruk Gül Merve Yalcin Nilay Er Gulnur Kizilay Ozfidan |
| author_sort | Gonca Duygu Çapar |
| collection | DOAJ |
| description | The aim of this study is to investigate the effect of doxycycline collagen sponge on bisphosphonate-related osteonecrosis of the jaw (BRONJ) and the level of serum biomarkers as an indicator of osteonecrosis. Twenty-four rats were divided into four groups. Animals in the control group were injected with saline and animals in Groups I, II and III were injected with zoledronate three times a week for eight weeks. After eight weeks, the following procedures were performed in each group. In Group I: extraction of maxillary first molar, in Group II: extraction of maxillary first molar and mucoperiosteal coverage was performed and in Group III: extraction of maxillary first molar and mucoperiosteal coverage with doxycycline collagen sponges was performed. At the end of 16 weeks, all animals were sacrificed. Serum collagen type I C-telopeptide (CTx), tartrate-resistant acid phosphatase (TRACP 5b) and alkaline phosphatase (ALP) levels’ analysis, clinical examination, histological and histomorphometrical analysis were performed. As a result no significant difference in CTx, TRACP 5b and ALP levels was observed between groups. Complete mucosal healing was observed in all animals in the control group and 66.7% of animals in Group III. The necrotic bone area in Group III was significantly lower than the other groups (p < 0.01). Statistically significant difference was observed between groups in terms of detached osteoclast number (p < 0.01). In conclusion, local application of doxycycline could have a positive effect in reducing the risk of BRONJ in rats. |
| first_indexed | 2024-12-10T20:35:29Z |
| format | Article |
| id | doaj.art-8d63e331e0774ad58d5e5d4badb72691 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-10T20:35:29Z |
| publishDate | 2016-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-8d63e331e0774ad58d5e5d4badb726912022-12-22T01:34:33ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-07-0130475276110.1080/13102818.2016.11740781174078Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal studyGonca Duygu Çapar0Melike Sapmaz-Metin1Esma Kütan2Ceyda Ozcakir Tomruk3Gül Merve Yalcin4Nilay Er5Gulnur Kizilay Ozfidan6Trakya UniversityTrakya UniversityYeditepe UniversityYeditepe UniversityOkan UniversityTrakya UniversityTrakya UniversityThe aim of this study is to investigate the effect of doxycycline collagen sponge on bisphosphonate-related osteonecrosis of the jaw (BRONJ) and the level of serum biomarkers as an indicator of osteonecrosis. Twenty-four rats were divided into four groups. Animals in the control group were injected with saline and animals in Groups I, II and III were injected with zoledronate three times a week for eight weeks. After eight weeks, the following procedures were performed in each group. In Group I: extraction of maxillary first molar, in Group II: extraction of maxillary first molar and mucoperiosteal coverage was performed and in Group III: extraction of maxillary first molar and mucoperiosteal coverage with doxycycline collagen sponges was performed. At the end of 16 weeks, all animals were sacrificed. Serum collagen type I C-telopeptide (CTx), tartrate-resistant acid phosphatase (TRACP 5b) and alkaline phosphatase (ALP) levels’ analysis, clinical examination, histological and histomorphometrical analysis were performed. As a result no significant difference in CTx, TRACP 5b and ALP levels was observed between groups. Complete mucosal healing was observed in all animals in the control group and 66.7% of animals in Group III. The necrotic bone area in Group III was significantly lower than the other groups (p < 0.01). Statistically significant difference was observed between groups in terms of detached osteoclast number (p < 0.01). In conclusion, local application of doxycycline could have a positive effect in reducing the risk of BRONJ in rats.http://dx.doi.org/10.1080/13102818.2016.1174078BRONJdoxycyclineCTxALPTRACP 5b |
| spellingShingle | Gonca Duygu Çapar Melike Sapmaz-Metin Esma Kütan Ceyda Ozcakir Tomruk Gül Merve Yalcin Nilay Er Gulnur Kizilay Ozfidan Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study Biotechnology & Biotechnological Equipment BRONJ doxycycline CTx ALP TRACP 5b |
| title | Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study |
| title_full | Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study |
| title_fullStr | Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study |
| title_full_unstemmed | Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study |
| title_short | Preventive effect of doxycycline sponge against bisphosphonate-related osteonecrosis of the jaws: an animal study |
| title_sort | preventive effect of doxycycline sponge against bisphosphonate related osteonecrosis of the jaws an animal study |
| topic | BRONJ doxycycline CTx ALP TRACP 5b |
| url | http://dx.doi.org/10.1080/13102818.2016.1174078 |
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