The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM
Abstract Strain-specific probiotics can present antioxidant activity and reduce damage caused by oxidation. Streptococcus alactolyticus strain FGM (S. alactolyticus strain FGM) isolated from the chicken cecum shows potential probiotic properties which have been previously demonstrated. However, the...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-58933-8 |
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| author | Xueyan Gu Heng Wang Lei Wang Kang Zhang Yuhu Tian Xiaoya Wang Guowei Xu Zhiting Guo Saad Ahmad Hanyurwumutima Egide Jiahui Liu Jianxi Li Huub F. J. Savelkoul Jingyan Zhang Xuezhi Wang |
| author_facet | Xueyan Gu Heng Wang Lei Wang Kang Zhang Yuhu Tian Xiaoya Wang Guowei Xu Zhiting Guo Saad Ahmad Hanyurwumutima Egide Jiahui Liu Jianxi Li Huub F. J. Savelkoul Jingyan Zhang Xuezhi Wang |
| author_sort | Xueyan Gu |
| collection | DOAJ |
| description | Abstract Strain-specific probiotics can present antioxidant activity and reduce damage caused by oxidation. Streptococcus alactolyticus strain FGM (S. alactolyticus strain FGM) isolated from the chicken cecum shows potential probiotic properties which have been previously demonstrated. However, the antioxidant properties of S. alactolyticus strain FGM remain unknown. In this view, cell-free supernatant (CFS), intact cells (IC) and intracellular extracts (CFE) of strain FGM and 3 strains of Lactobacillus (LAB) were prepared, and their scavenging capacities against DPPH, hydroxyl radicals and linoleic acid peroxidation inhibitory were compared in this study. The effects of strain FGM cell-free supernatant (FCFS) on NO production, activity of SOD and GSH-Px in RAW264.7 cells and LPS-induced RAW264.7 cells were analyzed. The metabolites in the supernatant were quantitated by N300 Quantitative Metabolome. It was shown that the physicochemical characteristics of CFS to scavenge DPPH, hydroxyl radicals, and linoleic acid peroxidation inhibitory were significantly stronger than that of IC and CFE in the strain FGM (P < 0.05), respectively 87.12% ± 1.62, 45.03% ± 1.27, 15.63% ± 1.34. FCFS had a promotional effect on RAW264.7 cells, and significantly elevated SOD and GSH-Px activities in RAW264.7 cells. 25 μL FCFS significantly promoted the proliferation of RAW264.7 cells induced by LPS, increased the activities of SOD and GSH-PX, and decreased the release of NO. Furthermore, among the differential metabolites of FCFS quantified by N300, 12 metabolites were significantly up-regulated, including lactic acid, indole lactic acid, linoleic acid, pyruvic acid etc., many of which are known with antioxidant properties. In conclusion, FCFS had good antioxidant properties and activity, which can be attributed to metabolites produced from strain FGM fermentation. It was further confirmed that S. alactolyticus strain FGM and its postbiotic have potential probiotic properties and bright application prospects in livestock and poultry breeding. |
| first_indexed | 2024-04-24T09:54:27Z |
| format | Article |
| id | doaj.art-8d66c7ed90a74923b955b2187e822cf7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T09:54:27Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-8d66c7ed90a74923b955b2187e822cf72024-04-14T11:12:51ZengNature PortfolioScientific Reports2045-23222024-04-0114111510.1038/s41598-024-58933-8The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGMXueyan Gu0Heng Wang1Lei Wang2Kang Zhang3Yuhu Tian4Xiaoya Wang5Guowei Xu6Zhiting Guo7Saad Ahmad8Hanyurwumutima Egide9Jiahui Liu10Jianxi Li11Huub F. J. Savelkoul12Jingyan Zhang13Xuezhi Wang14Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesCell Biology and Immunology Group, Wageningen University & ResearchEngineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural SciencesLanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract Strain-specific probiotics can present antioxidant activity and reduce damage caused by oxidation. Streptococcus alactolyticus strain FGM (S. alactolyticus strain FGM) isolated from the chicken cecum shows potential probiotic properties which have been previously demonstrated. However, the antioxidant properties of S. alactolyticus strain FGM remain unknown. In this view, cell-free supernatant (CFS), intact cells (IC) and intracellular extracts (CFE) of strain FGM and 3 strains of Lactobacillus (LAB) were prepared, and their scavenging capacities against DPPH, hydroxyl radicals and linoleic acid peroxidation inhibitory were compared in this study. The effects of strain FGM cell-free supernatant (FCFS) on NO production, activity of SOD and GSH-Px in RAW264.7 cells and LPS-induced RAW264.7 cells were analyzed. The metabolites in the supernatant were quantitated by N300 Quantitative Metabolome. It was shown that the physicochemical characteristics of CFS to scavenge DPPH, hydroxyl radicals, and linoleic acid peroxidation inhibitory were significantly stronger than that of IC and CFE in the strain FGM (P < 0.05), respectively 87.12% ± 1.62, 45.03% ± 1.27, 15.63% ± 1.34. FCFS had a promotional effect on RAW264.7 cells, and significantly elevated SOD and GSH-Px activities in RAW264.7 cells. 25 μL FCFS significantly promoted the proliferation of RAW264.7 cells induced by LPS, increased the activities of SOD and GSH-PX, and decreased the release of NO. Furthermore, among the differential metabolites of FCFS quantified by N300, 12 metabolites were significantly up-regulated, including lactic acid, indole lactic acid, linoleic acid, pyruvic acid etc., many of which are known with antioxidant properties. In conclusion, FCFS had good antioxidant properties and activity, which can be attributed to metabolites produced from strain FGM fermentation. It was further confirmed that S. alactolyticus strain FGM and its postbiotic have potential probiotic properties and bright application prospects in livestock and poultry breeding.https://doi.org/10.1038/s41598-024-58933-8S. alactolyticus strain FGMAntioxidant activityMetabolomic analysisSupernatantOxidative stress |
| spellingShingle | Xueyan Gu Heng Wang Lei Wang Kang Zhang Yuhu Tian Xiaoya Wang Guowei Xu Zhiting Guo Saad Ahmad Hanyurwumutima Egide Jiahui Liu Jianxi Li Huub F. J. Savelkoul Jingyan Zhang Xuezhi Wang The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM Scientific Reports S. alactolyticus strain FGM Antioxidant activity Metabolomic analysis Supernatant Oxidative stress |
| title | The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM |
| title_full | The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM |
| title_fullStr | The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM |
| title_full_unstemmed | The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM |
| title_short | The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM |
| title_sort | antioxidant activity and metabolomic analysis of the supernatant of streptococcus alactolyticus strain fgm |
| topic | S. alactolyticus strain FGM Antioxidant activity Metabolomic analysis Supernatant Oxidative stress |
| url | https://doi.org/10.1038/s41598-024-58933-8 |
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