Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs
Abstract Differences in total number of piglets born per litter are observed between the Norwegian Duroc (ND) sire and Norwegian Landrace (NL) dam line. The aim of this study was to evaluate ovarian characteristics, and in vitro nuclear and cytoplasmic oocyte maturation in both breeds. One day after...
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| Format: | Article |
| Language: | English |
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Wiley
2021-09-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.498 |
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| author | Reina Jochems Ann Helen Gaustad Louisa J. Zak Eli Grindflek Teklu T. Zeremichael Irma C. Oskam Frøydis D. Myromslien Elisabeth Kommisrud Anette K. Krogenæs |
| author_facet | Reina Jochems Ann Helen Gaustad Louisa J. Zak Eli Grindflek Teklu T. Zeremichael Irma C. Oskam Frøydis D. Myromslien Elisabeth Kommisrud Anette K. Krogenæs |
| author_sort | Reina Jochems |
| collection | DOAJ |
| description | Abstract Differences in total number of piglets born per litter are observed between the Norwegian Duroc (ND) sire and Norwegian Landrace (NL) dam line. The aim of this study was to evaluate ovarian characteristics, and in vitro nuclear and cytoplasmic oocyte maturation in both breeds. One day after weaning, follicular phase ovaries were collected. Ovary length and weight were measured and the number of follicles (< 3 mm and 3–8 mm) was counted. Cumulus‐oocyte complexes (COCs) were collected and matured for 48 hr. To assess cumulus expansion, COC area was analysed at 0 and 20 hr. Nuclear maturation and cortical granule (CG) distribution were analysed at 20 and 48 hr, and total glutathione (GSH) was measured at 48 hr to further elucidate cytoplasmic maturation. In first parity sows, a smaller ovary length and fewer 3 to 8 mm follicles were observed in ND compared to NL. For all sows, ND COCs covered a significantly smaller area at 0 hr, but a higher cumulus expansion ratio was observed at 20 hr compared to NL (364 ± 46% versus. 278 ± 27%, p < 0.001). At 20 hr, more ND oocytes exhibited advanced stages of nuclear maturation, while more NL oocytes showed advanced stages of CG distribution. Nuclear maturation to MII stage at 48 hr did not differ between ND and NL oocytes (90.1% and 87.7%, respectively). Moreover, no significant differences were observed for GSH content or CG distribution after maturation. In conclusion, differences with regard to ovarian characteristics as well as to cumulus expansion, and nuclear and cytoplasmic oocyte maturation at 20 hr were observed between the breeds. Further studies are required to determine if this subsequently affects in vitro fertilization and embryo development. |
| first_indexed | 2024-12-22T09:46:35Z |
| format | Article |
| id | doaj.art-8d686cc30d334fd6abccaa56ba82df75 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-12-22T09:46:35Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-8d686cc30d334fd6abccaa56ba82df752022-12-21T18:30:31ZengWileyVeterinary Medicine and Science2053-10952021-09-01751845185310.1002/vms3.498Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigsReina Jochems0Ann Helen Gaustad1Louisa J. Zak2Eli Grindflek3Teklu T. Zeremichael4Irma C. Oskam5Frøydis D. Myromslien6Elisabeth Kommisrud7Anette K. Krogenæs8Norsvin SA Hamar NorwayNorsvin SA Hamar NorwayTopigs Norsvin Research Center Beuningen The NetherlandsNorsvin SA Hamar NorwayDepartment of Biotechnology Inland Norway University of Applied Sciences Hamar NorwayThe Animal Production Experimental Centre Norwegian University of Life Sciences Ås NorwayDepartment of Biotechnology Inland Norway University of Applied Sciences Hamar NorwayDepartment of Biotechnology Inland Norway University of Applied Sciences Hamar NorwayFaculty of Veterinary Medicine Norwegian University of Life Sciences Oslo NorwayAbstract Differences in total number of piglets born per litter are observed between the Norwegian Duroc (ND) sire and Norwegian Landrace (NL) dam line. The aim of this study was to evaluate ovarian characteristics, and in vitro nuclear and cytoplasmic oocyte maturation in both breeds. One day after weaning, follicular phase ovaries were collected. Ovary length and weight were measured and the number of follicles (< 3 mm and 3–8 mm) was counted. Cumulus‐oocyte complexes (COCs) were collected and matured for 48 hr. To assess cumulus expansion, COC area was analysed at 0 and 20 hr. Nuclear maturation and cortical granule (CG) distribution were analysed at 20 and 48 hr, and total glutathione (GSH) was measured at 48 hr to further elucidate cytoplasmic maturation. In first parity sows, a smaller ovary length and fewer 3 to 8 mm follicles were observed in ND compared to NL. For all sows, ND COCs covered a significantly smaller area at 0 hr, but a higher cumulus expansion ratio was observed at 20 hr compared to NL (364 ± 46% versus. 278 ± 27%, p < 0.001). At 20 hr, more ND oocytes exhibited advanced stages of nuclear maturation, while more NL oocytes showed advanced stages of CG distribution. Nuclear maturation to MII stage at 48 hr did not differ between ND and NL oocytes (90.1% and 87.7%, respectively). Moreover, no significant differences were observed for GSH content or CG distribution after maturation. In conclusion, differences with regard to ovarian characteristics as well as to cumulus expansion, and nuclear and cytoplasmic oocyte maturation at 20 hr were observed between the breeds. Further studies are required to determine if this subsequently affects in vitro fertilization and embryo development.https://doi.org/10.1002/vms3.498breed differencesDurocin vitro oocyte maturationLandraceovaryporcine |
| spellingShingle | Reina Jochems Ann Helen Gaustad Louisa J. Zak Eli Grindflek Teklu T. Zeremichael Irma C. Oskam Frøydis D. Myromslien Elisabeth Kommisrud Anette K. Krogenæs Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs Veterinary Medicine and Science breed differences Duroc in vitro oocyte maturation Landrace ovary porcine |
| title | Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs |
| title_full | Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs |
| title_fullStr | Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs |
| title_full_unstemmed | Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs |
| title_short | Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs |
| title_sort | ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in duroc and landrace pigs |
| topic | breed differences Duroc in vitro oocyte maturation Landrace ovary porcine |
| url | https://doi.org/10.1002/vms3.498 |
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