Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice
This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2009-03-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100016 |
| _version_ | 1819024048185671680 |
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| author | Dalva Assunção Portari Mancini Rosângela Pavan Torres José Ricardo Pinto Jorge Mancini-Filho |
| author_facet | Dalva Assunção Portari Mancini Rosângela Pavan Torres José Ricardo Pinto Jorge Mancini-Filho |
| author_sort | Dalva Assunção Portari Mancini |
| collection | DOAJ |
| description | This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID50/mL of the HSV-1 was kept for 3 h at 4º C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37º C in CO2-5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The HSV-1 replication inhibition percentage (IP) measured the antiviral action from antioxidants, showing viral reductions of the 82.0, 82.5%, in the presence of rosemary and rosemary + BHT, respectively. As an extension, cell test corresponded to the similar viral decrease (IP = 85.0 and 86.3%) in both aforementioned situations. Results lead to conclude that phenolic compounds from rosemary revealed an antiviral action on herpesvirus-1.<br>Neste estudo foi avaliada a ação antiviral de antioxidantes de especiaria. Extrato aquoso de alecrim (Rosmarinus officinalis, L), que apresentou atividade antioxidante através de espectrofotometria usando o sistema β caroteno/ácido linoléico, foi avaliado em ensaios com vírus herpes-1 na replicação em células VERO. Nestes ensaios foram utilizados 10.000 TCID50%/mL do vírus HSV-1, mantidos em contato com 300 ppm do extrato de alecrim e com 100 ppm de butil hidroxi tolueno (BHT), durante 3h a 4°C. Esses vírus, em seguida, foram inoculados em células VERO incubadas a 37 °C/5% de CO2 por sete dias. Pelo efeito citopático (ECP) e o "end point" de ECP do controle de vírus (sem antioxidante), foi possível observar que houve reduções na replicação viral de 82 e 82,5% na presença do alecrim e do alecrim + BHT, respectivamente. Nessa situação,avaliou-se ainda a redução da adsorção viral às células, que apresentou índices similares de 85,0 e 86,3% de redução na capacidade da adsorção. Estas reduções no desempenho do HSV foram medidas pela fórmula de porcentagem de inibição da replicação viral (PI). Os resultados levam a concluir que os compostos fenólicos do alecrim apresentam ação antiviral sobre o HSV-1. |
| first_indexed | 2024-12-21T04:48:35Z |
| format | Article |
| id | doaj.art-8d74030798c342a6b12f3cb57f79082d |
| institution | Directory Open Access Journal |
| issn | 1984-8250 2175-9790 |
| language | English |
| last_indexed | 2024-12-21T04:48:35Z |
| publishDate | 2009-03-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-8d74030798c342a6b12f3cb57f79082d2022-12-21T19:15:31ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences1984-82502175-97902009-03-0145112713310.1590/S1984-82502009000100016Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spiceDalva Assunção Portari ManciniRosângela Pavan TorresJosé Ricardo PintoJorge Mancini-FilhoThis work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID50/mL of the HSV-1 was kept for 3 h at 4º C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37º C in CO2-5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The HSV-1 replication inhibition percentage (IP) measured the antiviral action from antioxidants, showing viral reductions of the 82.0, 82.5%, in the presence of rosemary and rosemary + BHT, respectively. As an extension, cell test corresponded to the similar viral decrease (IP = 85.0 and 86.3%) in both aforementioned situations. Results lead to conclude that phenolic compounds from rosemary revealed an antiviral action on herpesvirus-1.<br>Neste estudo foi avaliada a ação antiviral de antioxidantes de especiaria. Extrato aquoso de alecrim (Rosmarinus officinalis, L), que apresentou atividade antioxidante através de espectrofotometria usando o sistema β caroteno/ácido linoléico, foi avaliado em ensaios com vírus herpes-1 na replicação em células VERO. Nestes ensaios foram utilizados 10.000 TCID50%/mL do vírus HSV-1, mantidos em contato com 300 ppm do extrato de alecrim e com 100 ppm de butil hidroxi tolueno (BHT), durante 3h a 4°C. Esses vírus, em seguida, foram inoculados em células VERO incubadas a 37 °C/5% de CO2 por sete dias. Pelo efeito citopático (ECP) e o "end point" de ECP do controle de vírus (sem antioxidante), foi possível observar que houve reduções na replicação viral de 82 e 82,5% na presença do alecrim e do alecrim + BHT, respectivamente. Nessa situação,avaliou-se ainda a redução da adsorção viral às células, que apresentou índices similares de 85,0 e 86,3% de redução na capacidade da adsorção. Estas reduções no desempenho do HSV foram medidas pela fórmula de porcentagem de inibição da replicação viral (PI). Os resultados levam a concluir que os compostos fenólicos do alecrim apresentam ação antiviral sobre o HSV-1.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100016Antioxidantes naturaisReplicação viralCompostos fenólicosVírus herpesAlecrimNatural antioxidantsVirus replicationPhenolic compoundsHerpesvirusRosemary |
| spellingShingle | Dalva Assunção Portari Mancini Rosângela Pavan Torres José Ricardo Pinto Jorge Mancini-Filho Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice Brazilian Journal of Pharmaceutical Sciences Antioxidantes naturais Replicação viral Compostos fenólicos Vírus herpes Alecrim Natural antioxidants Virus replication Phenolic compounds Herpesvirus Rosemary |
| title | Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice |
| title_full | Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice |
| title_fullStr | Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice |
| title_full_unstemmed | Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice |
| title_short | Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice |
| title_sort | inhibition of dna virus herpes 1 hsv 1 in cellular culture replication through an antioxidant treatment extracted from rosemary spice |
| topic | Antioxidantes naturais Replicação viral Compostos fenólicos Vírus herpes Alecrim Natural antioxidants Virus replication Phenolic compounds Herpesvirus Rosemary |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100016 |
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