Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells
Long non-coding RNAs (lncRNAs) have been proved to have an important role in different malignancies including clear cell renal cell carcinoma (ccRCC). However, their role in disease progression is still not clear. The objective of the study was to identify lncRNA-based prognostic biomarkers and furt...
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PeerJ Inc.
2020-10-01
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| Online Access: | https://peerj.com/articles/10149.pdf |
| _version_ | 1797424693072887808 |
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| author | Feilong Yang Cheng Liu Guojiang Zhao Liyuan Ge Yimeng Song Zhigang Chen Zhuo Liu Kai Hong Lulin Ma |
| author_facet | Feilong Yang Cheng Liu Guojiang Zhao Liyuan Ge Yimeng Song Zhigang Chen Zhuo Liu Kai Hong Lulin Ma |
| author_sort | Feilong Yang |
| collection | DOAJ |
| description | Long non-coding RNAs (lncRNAs) have been proved to have an important role in different malignancies including clear cell renal cell carcinoma (ccRCC). However, their role in disease progression is still not clear. The objective of the study was to identify lncRNA-based prognostic biomarkers and further to investigate the role of one lncRNA LINC01234 in progression of ccRCC cells. We found that six adverse prognostic lncRNA biomarkers including LINC01234 were identified in ccRCC patients by bioinformatic analysis using The Cancer Genome Atlas database. LINC01234 knockdown impaired cell proliferation, migration and invasion in vitro as compared to negative control. Furthermore, the epithelial-mesenchymal transition was inhibited after LINC01234 knockdown. Additionally, LINC01234 knockdown impaired hypoxia-inducible factor-2a (HIF-2α) pathways, including a suppression of the expression of HIF-2α, vascular endothelial growth factor A, epidermal growth factor receptor, c-Myc, Cyclin D1 and MET. Together, these datas showed that LINC01234 was likely to regulate the progression of ccRCC by HIF-2α pathways, and LINC01234 was both a promising prognostic biomarker and a potential therapeutic target for ccRCC. |
| first_indexed | 2024-03-09T08:04:33Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:04:33Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-8d76cfc81ff5443ca60260e4f13db1ae2023-12-03T00:23:54ZengPeerJ Inc.PeerJ2167-83592020-10-018e1014910.7717/peerj.10149Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cellsFeilong YangCheng LiuGuojiang ZhaoLiyuan GeYimeng SongZhigang ChenZhuo LiuKai HongLulin MaLong non-coding RNAs (lncRNAs) have been proved to have an important role in different malignancies including clear cell renal cell carcinoma (ccRCC). However, their role in disease progression is still not clear. The objective of the study was to identify lncRNA-based prognostic biomarkers and further to investigate the role of one lncRNA LINC01234 in progression of ccRCC cells. We found that six adverse prognostic lncRNA biomarkers including LINC01234 were identified in ccRCC patients by bioinformatic analysis using The Cancer Genome Atlas database. LINC01234 knockdown impaired cell proliferation, migration and invasion in vitro as compared to negative control. Furthermore, the epithelial-mesenchymal transition was inhibited after LINC01234 knockdown. Additionally, LINC01234 knockdown impaired hypoxia-inducible factor-2a (HIF-2α) pathways, including a suppression of the expression of HIF-2α, vascular endothelial growth factor A, epidermal growth factor receptor, c-Myc, Cyclin D1 and MET. Together, these datas showed that LINC01234 was likely to regulate the progression of ccRCC by HIF-2α pathways, and LINC01234 was both a promising prognostic biomarker and a potential therapeutic target for ccRCC.https://peerj.com/articles/10149.pdfBioinformaticslncRNALINC01234Clear cell renal cell carcinomaEMTHIF-2α pathways |
| spellingShingle | Feilong Yang Cheng Liu Guojiang Zhao Liyuan Ge Yimeng Song Zhigang Chen Zhuo Liu Kai Hong Lulin Ma Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells PeerJ Bioinformatics lncRNA LINC01234 Clear cell renal cell carcinoma EMT HIF-2α pathways |
| title | Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells |
| title_full | Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells |
| title_fullStr | Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells |
| title_full_unstemmed | Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells |
| title_short | Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells |
| title_sort | long non coding rna linc01234 regulates proliferation migration and invasion via hif 2α pathways in clear cell renal cell carcinoma cells |
| topic | Bioinformatics lncRNA LINC01234 Clear cell renal cell carcinoma EMT HIF-2α pathways |
| url | https://peerj.com/articles/10149.pdf |
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