Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice
Summary: Recurrent seizures are a common feature in many neurologic disorders. Seizure examination may help with diagnosis, preclinical study, and development of treatment strategies. Here we detail protocols to prepare and implant electrodes, as well as to record and analyze seizure events in freel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722001253 |
| _version_ | 1818992482440970240 |
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| author | Qi Wang Nicola de Prisco Jianrong Tang Vincenzo A. Gennarino |
| author_facet | Qi Wang Nicola de Prisco Jianrong Tang Vincenzo A. Gennarino |
| author_sort | Qi Wang |
| collection | DOAJ |
| description | Summary: Recurrent seizures are a common feature in many neurologic disorders. Seizure examination may help with diagnosis, preclinical study, and development of treatment strategies. Here we detail protocols to prepare and implant electrodes, as well as to record and analyze seizure events in freely moving mice. SCA47 mice exhibit both preclinical seizures (i.e., epileptiform discharges of EEG) starting from ∼14 weeks of age and behavioral seizures (i.e., spontaneous behavioral seizures) starting from ∼22 weeks of age.For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018). |
| first_indexed | 2024-12-20T20:26:52Z |
| format | Article |
| id | doaj.art-8d77471b4ca744df9cdb1b78160c3382 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-20T20:26:52Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8d77471b4ca744df9cdb1b78160c33822022-12-21T19:27:28ZengElsevierSTAR Protocols2666-16672022-06-0132101245Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving miceQi Wang0Nicola de Prisco1Jianrong Tang2Vincenzo A. Gennarino3Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, TX 77030, USA; Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USADepartment of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USAJan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, TX 77030, USA; Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA; Corresponding authorDepartment of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA; Departments of Pediatrics and Neurology, Columbia University Irving Medical Center, New York, NY 10032, USA; Columbia Stem Cell Initiative, Columbia University Irving Medical Center, New York, NY 10032, USA; Initiative for Columbia Ataxia and Tremor, Columbia University Irving Medical Center, New York, NY 10032, USA; Corresponding authorSummary: Recurrent seizures are a common feature in many neurologic disorders. Seizure examination may help with diagnosis, preclinical study, and development of treatment strategies. Here we detail protocols to prepare and implant electrodes, as well as to record and analyze seizure events in freely moving mice. SCA47 mice exhibit both preclinical seizures (i.e., epileptiform discharges of EEG) starting from ∼14 weeks of age and behavioral seizures (i.e., spontaneous behavioral seizures) starting from ∼22 weeks of age.For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018).http://www.sciencedirect.com/science/article/pii/S2666166722001253GeneticsModel OrganismsNeuroscienceBehavior |
| spellingShingle | Qi Wang Nicola de Prisco Jianrong Tang Vincenzo A. Gennarino Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice STAR Protocols Genetics Model Organisms Neuroscience Behavior |
| title | Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice |
| title_full | Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice |
| title_fullStr | Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice |
| title_full_unstemmed | Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice |
| title_short | Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice |
| title_sort | protocol for recording epileptiform discharges of eeg and behavioral seizures in freely moving mice |
| topic | Genetics Model Organisms Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722001253 |
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