Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis

Antibacterial drugs are routinely used in therapy of periodontal diseases. However, increasing incidence of antibiotics resistance necessitates development of novel therapeutic approaches for oral diseases. Currently, newly designed antibacterial agents based on natural, semi-synthetic and synthetic...

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Main Authors: N. G. Sarkisian, N. N. Kataeva, I. A. Tuzankina, S. G. Melikyan, V. A. Zurochka, A. V. Zurochka
Format: Article
Language:Russian
Published: Sankt-Peterburg : NIIÈM imeni Pastera 2019-11-01
Series:Инфекция и иммунитет
Subjects:
Online Access:https://www.iimmun.ru/iimm/article/view/766
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author N. G. Sarkisian
N. N. Kataeva
I. A. Tuzankina
S. G. Melikyan
V. A. Zurochka
A. V. Zurochka
author_facet N. G. Sarkisian
N. N. Kataeva
I. A. Tuzankina
S. G. Melikyan
V. A. Zurochka
A. V. Zurochka
author_sort N. G. Sarkisian
collection DOAJ
description Antibacterial drugs are routinely used in therapy of periodontal diseases. However, increasing incidence of antibiotics resistance necessitates development of novel therapeutic approaches for oral diseases. Currently, newly designed antibacterial agents based on natural, semi-synthetic and synthetic peptides is the most promising approach in dentistry. Among them is a product containing synthetic peptide (ZP2) replicating active site of granulocyte-macrophage colonystimulating factor (GM-CSF) as the main active ingredient in Atsegram-spray (manufactured by Academic Innovation Research Center, Chelyabinsk). Our study was aimed at assessing efficacy of a combination therapy of inflammatory periodontal diseases by introducing Atsegram-spray as well as examining potential relationship between peptide ZP2-related biological properties, physicochemical properties of the spray and mechanism of antibacterial and immunotropic action for substantiating its application on oral mucosa. During the first stage experiments, it was found that the peptide ZP2 was able to trigger lymphocyte blast transformation in vitro indicating that it might influence cell proliferation and exhibit marked immunotropic activity. Next, we assessed potential effects of the peptide ZP2 on biofilm formation mediated by staphylococcal clinical isolates. It was shown that peptide ZP2 inhibited biofilm formation in 75.0±9.0% of S. aureus and 50.0±15.1% of S. epidermidis strains, with mean inhibition index of biofilm formation reaching 25.1±3.8 and 50.4±6.0%, respectively. However, peptide ZP2 in 8.3–25.0% of staphylococcal clinical isolates was found to stimulate/lack effect on biofilm formation by 14.9–48.5 and 16.7–25.0% cultures, respectively. Thus, the synthetic peptide ZP2 exerts divergent, but mainly inhibitory effects on biofilm formation with staphylococcal clinical strains, which are characterized by inter- and intraspecific (strain) variability. Use of a synthetic peptide-based spray in antibacterial therapy of mild chronic generalized periodontitis (main group) one month after the onset was found to improve oral hygiene by 28.5% as well as decrease PMA index and gum bleeding index by 82.8 and 100%, respectively. In contrast, such parameters in patients receiving basic therapy (comparison group) were lower on average by 2-fold. While analyzing physicochemical properties of the spray such as pH, buffer capacity and solution osmotic pressure, it was found that they were related to the antibacterial mechanism of drug activity and efficacy in treatment of inflammatory periodontal diseases. Thus, assessing peptide ZP2-related biological properties and physicochemical parameters of the spray allows to evaluate their role in mechanism of previously unknown antibacterial and immunotropic activity. These findings confirm feasibility and efficacy of using Atsegram-spray in dentistry as an alternative means to antimicrobial agents, such as antibacterial drugs.
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spelling doaj.art-8d811d45d83c40139927947a40e1b9872023-01-02T19:35:48ZrusSankt-Peterburg : NIIÈM imeni PasteraИнфекция и иммунитет2220-76192313-73982019-11-0193-454955810.15789/2220-7619-2019-3-4-549-558782Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitisN. G. Sarkisian0N. N. Kataeva1I. A. Tuzankina2S. G. Melikyan3V. A. Zurochka4A. V. Zurochka5Ural State Medical University of the Ministry of Health of the Russian Federation, Yekaterinburg Institute of Immunology and Physiology Ural detachment of the Russian Academy of Sciences, YekaterinburgUral State Medical University of the Ministry of Health of the Russian FederationInstitute of Immunology and Physiology Ural detachment of the Russian Academy of SciencesUral State Medical University of the Ministry of Health of the Russian FederationInstitute of Immunology and Physiology Ural detachment of the Russian Academy of Sciences; South Ural State University (National Research University)Institute of Immunology and Physiology Ural detachment of the Russian Academy of Sciences; South Ural State University (National Research University)Antibacterial drugs are routinely used in therapy of periodontal diseases. However, increasing incidence of antibiotics resistance necessitates development of novel therapeutic approaches for oral diseases. Currently, newly designed antibacterial agents based on natural, semi-synthetic and synthetic peptides is the most promising approach in dentistry. Among them is a product containing synthetic peptide (ZP2) replicating active site of granulocyte-macrophage colonystimulating factor (GM-CSF) as the main active ingredient in Atsegram-spray (manufactured by Academic Innovation Research Center, Chelyabinsk). Our study was aimed at assessing efficacy of a combination therapy of inflammatory periodontal diseases by introducing Atsegram-spray as well as examining potential relationship between peptide ZP2-related biological properties, physicochemical properties of the spray and mechanism of antibacterial and immunotropic action for substantiating its application on oral mucosa. During the first stage experiments, it was found that the peptide ZP2 was able to trigger lymphocyte blast transformation in vitro indicating that it might influence cell proliferation and exhibit marked immunotropic activity. Next, we assessed potential effects of the peptide ZP2 on biofilm formation mediated by staphylococcal clinical isolates. It was shown that peptide ZP2 inhibited biofilm formation in 75.0±9.0% of S. aureus and 50.0±15.1% of S. epidermidis strains, with mean inhibition index of biofilm formation reaching 25.1±3.8 and 50.4±6.0%, respectively. However, peptide ZP2 in 8.3–25.0% of staphylococcal clinical isolates was found to stimulate/lack effect on biofilm formation by 14.9–48.5 and 16.7–25.0% cultures, respectively. Thus, the synthetic peptide ZP2 exerts divergent, but mainly inhibitory effects on biofilm formation with staphylococcal clinical strains, which are characterized by inter- and intraspecific (strain) variability. Use of a synthetic peptide-based spray in antibacterial therapy of mild chronic generalized periodontitis (main group) one month after the onset was found to improve oral hygiene by 28.5% as well as decrease PMA index and gum bleeding index by 82.8 and 100%, respectively. In contrast, such parameters in patients receiving basic therapy (comparison group) were lower on average by 2-fold. While analyzing physicochemical properties of the spray such as pH, buffer capacity and solution osmotic pressure, it was found that they were related to the antibacterial mechanism of drug activity and efficacy in treatment of inflammatory periodontal diseases. Thus, assessing peptide ZP2-related biological properties and physicochemical parameters of the spray allows to evaluate their role in mechanism of previously unknown antibacterial and immunotropic activity. These findings confirm feasibility and efficacy of using Atsegram-spray in dentistry as an alternative means to antimicrobial agents, such as antibacterial drugs.https://www.iimmun.ru/iimm/article/view/766periodontal diseasesynthetic peptideimmunotropic activityphysical and chemical propertiesantibacterial effect
spellingShingle N. G. Sarkisian
N. N. Kataeva
I. A. Tuzankina
S. G. Melikyan
V. A. Zurochka
A. V. Zurochka
Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis
Инфекция и иммунитет
periodontal disease
synthetic peptide
immunotropic activity
physical and chemical properties
antibacterial effect
title Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis
title_full Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis
title_fullStr Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis
title_full_unstemmed Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis
title_short Assessing efficiency of synthetic peptide-containing spray in combination therapy of chronic generalized periodontitis
title_sort assessing efficiency of synthetic peptide containing spray in combination therapy of chronic generalized periodontitis
topic periodontal disease
synthetic peptide
immunotropic activity
physical and chemical properties
antibacterial effect
url https://www.iimmun.ru/iimm/article/view/766
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