| Summary: | <p/> <p>The antibacterial effect of lactoferrin (Lf) was tested on isolates of <it>Escherichia coli (E. coli), Staphylococcus aureus (S. aureus)</it>, and coagulase-negative staphylococci (CNS) as well as on <it>Pseudomonas aeruginosa (P. aeruginosa) </it>and <it>Klebsiella pneumoniae (K. pneumoniae)</it>, originally isolated from bovine mastitis. Concentrations of Lf used were 0.67 mg/ml, 1.67 mg/ml, and 2.67 mg/ml. Growth of udder pathogens was monitored by turbidometry either in broth culture or in whey prepared from normal milk. We focused on 3 different growth variables: lag time, slope, and maximum absorbance of bacterial growth curves. Growth inhibition was seen in the broth but hardly at all in whey. The isolates of <it>E. coli </it>and CNS did not grow sufficiently well in whey to draw any conclusions. The most effective inhibitory activity of Lf was seen against <it>E. coli </it>and <it>P. aeruginosa</it>. All 5 <it>E. coli </it>isolates had similar growth patterns. Inhibition of growth by Lf was concentration-dependent. The concentration of 0.67 mg/ml in broth and whey was generally too low for a significant inhibitory effect.</p>
|
|---|