Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens
<p/> <p>The antibacterial effect of lactoferrin (Lf) was tested on isolates of <it>Escherichia coli (E. coli), Staphylococcus aureus (S. aureus)</it>, and coagulase-negative staphylococci (CNS) as well as on <it>Pseudomonas aeruginosa (P. aeruginosa) </it>and <...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-03-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://www.actavetscand.com/content/44/1/35 |
| _version_ | 1828310344601174016 |
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| author | Kaartinen L Saloniemi H Pyörälä S Kutila T |
| author_facet | Kaartinen L Saloniemi H Pyörälä S Kutila T |
| author_sort | Kaartinen L |
| collection | DOAJ |
| description | <p/> <p>The antibacterial effect of lactoferrin (Lf) was tested on isolates of <it>Escherichia coli (E. coli), Staphylococcus aureus (S. aureus)</it>, and coagulase-negative staphylococci (CNS) as well as on <it>Pseudomonas aeruginosa (P. aeruginosa) </it>and <it>Klebsiella pneumoniae (K. pneumoniae)</it>, originally isolated from bovine mastitis. Concentrations of Lf used were 0.67 mg/ml, 1.67 mg/ml, and 2.67 mg/ml. Growth of udder pathogens was monitored by turbidometry either in broth culture or in whey prepared from normal milk. We focused on 3 different growth variables: lag time, slope, and maximum absorbance of bacterial growth curves. Growth inhibition was seen in the broth but hardly at all in whey. The isolates of <it>E. coli </it>and CNS did not grow sufficiently well in whey to draw any conclusions. The most effective inhibitory activity of Lf was seen against <it>E. coli </it>and <it>P. aeruginosa</it>. All 5 <it>E. coli </it>isolates had similar growth patterns. Inhibition of growth by Lf was concentration-dependent. The concentration of 0.67 mg/ml in broth and whey was generally too low for a significant inhibitory effect.</p> |
| first_indexed | 2024-04-13T15:43:12Z |
| format | Article |
| id | doaj.art-8d8189df3f284480a7078b15eb423c87 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-04-13T15:43:12Z |
| publishDate | 2003-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-8d8189df3f284480a7078b15eb423c872022-12-22T02:41:05ZengBMCActa Veterinaria Scandinavica1751-01472003-03-01441354210.1186/1751-0147-44-35Antibacterial Effect of Bovine Lactoferrin Against Udder PathogensKaartinen LSaloniemi HPyörälä SKutila T<p/> <p>The antibacterial effect of lactoferrin (Lf) was tested on isolates of <it>Escherichia coli (E. coli), Staphylococcus aureus (S. aureus)</it>, and coagulase-negative staphylococci (CNS) as well as on <it>Pseudomonas aeruginosa (P. aeruginosa) </it>and <it>Klebsiella pneumoniae (K. pneumoniae)</it>, originally isolated from bovine mastitis. Concentrations of Lf used were 0.67 mg/ml, 1.67 mg/ml, and 2.67 mg/ml. Growth of udder pathogens was monitored by turbidometry either in broth culture or in whey prepared from normal milk. We focused on 3 different growth variables: lag time, slope, and maximum absorbance of bacterial growth curves. Growth inhibition was seen in the broth but hardly at all in whey. The isolates of <it>E. coli </it>and CNS did not grow sufficiently well in whey to draw any conclusions. The most effective inhibitory activity of Lf was seen against <it>E. coli </it>and <it>P. aeruginosa</it>. All 5 <it>E. coli </it>isolates had similar growth patterns. Inhibition of growth by Lf was concentration-dependent. The concentration of 0.67 mg/ml in broth and whey was generally too low for a significant inhibitory effect.</p>http://www.actavetscand.com/content/44/1/35mastitis pathogensgrowth inhibition |
| spellingShingle | Kaartinen L Saloniemi H Pyörälä S Kutila T Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens Acta Veterinaria Scandinavica mastitis pathogens growth inhibition |
| title | Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens |
| title_full | Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens |
| title_fullStr | Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens |
| title_full_unstemmed | Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens |
| title_short | Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens |
| title_sort | antibacterial effect of bovine lactoferrin against udder pathogens |
| topic | mastitis pathogens growth inhibition |
| url | http://www.actavetscand.com/content/44/1/35 |
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