Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2011-10-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019 |
| _version_ | 1811203284739817472 |
|---|---|
| author | Antonio Martins Oliveira Pedro de Oliva Neto |
| author_facet | Antonio Martins Oliveira Pedro de Oliva Neto |
| author_sort | Antonio Martins Oliveira |
| collection | DOAJ |
| description | The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. On the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and with short time, thus usable for industrial purpose to obtain more valuable products such as yeast extract enriched in RNA and/or protein, for different applications. |
| first_indexed | 2024-04-12T02:53:00Z |
| format | Article |
| id | doaj.art-8d83386571cd470dbd73b00d76abc89a |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-04-12T02:53:00Z |
| publishDate | 2011-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-8d83386571cd470dbd73b00d76abc89a2022-12-22T03:50:56ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242011-10-015451007101810.1590/S1516-89132011000500019Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysisAntonio Martins OliveiraPedro de Oliva NetoThe optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. On the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and with short time, thus usable for industrial purpose to obtain more valuable products such as yeast extract enriched in RNA and/or protein, for different applications.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019AutolysisNaCl plasmolysisyeast extractRNA extractionnucleotides |
| spellingShingle | Antonio Martins Oliveira Pedro de Oliva Neto Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis Brazilian Archives of Biology and Technology Autolysis NaCl plasmolysis yeast extract RNA extraction nucleotides |
| title | Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis |
| title_full | Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis |
| title_fullStr | Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis |
| title_full_unstemmed | Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis |
| title_short | Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis |
| title_sort | improvement in rna extraction from s cerevisie by optimization in the autolysis and nh3 hydrolysis |
| topic | Autolysis NaCl plasmolysis yeast extract RNA extraction nucleotides |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019 |
| work_keys_str_mv | AT antoniomartinsoliveira improvementinrnaextractionfromscerevisiebyoptimizationintheautolysisandnh3hydrolysis AT pedrodeolivaneto improvementinrnaextractionfromscerevisiebyoptimizationintheautolysisandnh3hydrolysis |