The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i>
Glycine- and proline-rich proteins (GPRPs) comprise a small conserved family that is widely distributed in the plant kingdom. GPRPs are relatively short peptides (<200 amino acids) that contain three typical domains, including an N-terminal XYPP-repeat domain, a middle hydrophobic domain rich in...
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/17/6168 |
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| author | Xiaojing Liu Xin Wang Xin Yan Shaobo Li Hui Peng |
| author_facet | Xiaojing Liu Xin Wang Xin Yan Shaobo Li Hui Peng |
| author_sort | Xiaojing Liu |
| collection | DOAJ |
| description | Glycine- and proline-rich proteins (GPRPs) comprise a small conserved family that is widely distributed in the plant kingdom. GPRPs are relatively short peptides (<200 amino acids) that contain three typical domains, including an N-terminal XYPP-repeat domain, a middle hydrophobic domain rich in alanine, and a C-terminal HGK-repeat domain. These proteins have been proposed to play fundamental roles in plant growth and environmental adaptation, but their functions remain unknown. In this study, we selected an <i>Arabidopsis</i> GPRP (AtGPRP3) to profile the physiological role of GPRPs. Transcripts of <i>AtGPRP3</i> could be detected in the whole <i>Arabidopsis</i> plant, but greater amounts were found in the rosette, followed by the cauline. The AtGPRP3::GFP fusion protein was mainly localized in the nucleus. The overexpression and knockout of <i>AtGPRP3</i>, respectively, retarded and accelerated the growth of <i>Arabidopsis</i> seedlings, while the increase in the growth rate of <i>atgprp3</i> plants was offset by the complementary expression of <i>AtGPRP3</i>. CAT2 and CAT3, but not CAT1, interacted with AtGPRP3 in the nuclei of <i>Arabidopsis</i> protoplasts. The knockout of <i>CAT2</i> by CRISPR-Cas9 retarded the growth of the <i>Arabidopsis</i> seedlings. Together, our data suggest that AtGPRP3 negatively regulates plant growth, potentially through CAT2 and CAT3. |
| first_indexed | 2024-03-10T16:47:59Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:47:59Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8d87150453be440884f0b2f24660807d2023-11-20T11:28:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117616810.3390/ijms21176168The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i>Xiaojing Liu0Xin Wang1Xin Yan2Shaobo Li3Hui Peng4Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang 330031, ChinaKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang 330031, ChinaKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang 330031, ChinaKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang 330031, ChinaThe Genome Center and Department of Plant Sciences, University of California, Davis, CA 95616, USAGlycine- and proline-rich proteins (GPRPs) comprise a small conserved family that is widely distributed in the plant kingdom. GPRPs are relatively short peptides (<200 amino acids) that contain three typical domains, including an N-terminal XYPP-repeat domain, a middle hydrophobic domain rich in alanine, and a C-terminal HGK-repeat domain. These proteins have been proposed to play fundamental roles in plant growth and environmental adaptation, but their functions remain unknown. In this study, we selected an <i>Arabidopsis</i> GPRP (AtGPRP3) to profile the physiological role of GPRPs. Transcripts of <i>AtGPRP3</i> could be detected in the whole <i>Arabidopsis</i> plant, but greater amounts were found in the rosette, followed by the cauline. The AtGPRP3::GFP fusion protein was mainly localized in the nucleus. The overexpression and knockout of <i>AtGPRP3</i>, respectively, retarded and accelerated the growth of <i>Arabidopsis</i> seedlings, while the increase in the growth rate of <i>atgprp3</i> plants was offset by the complementary expression of <i>AtGPRP3</i>. CAT2 and CAT3, but not CAT1, interacted with AtGPRP3 in the nuclei of <i>Arabidopsis</i> protoplasts. The knockout of <i>CAT2</i> by CRISPR-Cas9 retarded the growth of the <i>Arabidopsis</i> seedlings. Together, our data suggest that AtGPRP3 negatively regulates plant growth, potentially through CAT2 and CAT3.https://www.mdpi.com/1422-0067/21/17/6168<i>Arabidopsis</i>glycine- and proline-rich proteinprotein interactionseedling growth |
| spellingShingle | Xiaojing Liu Xin Wang Xin Yan Shaobo Li Hui Peng The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i> International Journal of Molecular Sciences <i>Arabidopsis</i> glycine- and proline-rich protein protein interaction seedling growth |
| title | The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i> |
| title_full | The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i> |
| title_fullStr | The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i> |
| title_full_unstemmed | The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i> |
| title_short | The Glycine- and Proline-Rich Protein AtGPRP3 Negatively Regulates Plant Growth in <i>Arabidopsis</i> |
| title_sort | glycine and proline rich protein atgprp3 negatively regulates plant growth in i arabidopsis i |
| topic | <i>Arabidopsis</i> glycine- and proline-rich protein protein interaction seedling growth |
| url | https://www.mdpi.com/1422-0067/21/17/6168 |
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