Background signals in stimulated Raman scattering microscopy and current solutions to avoid them
ABSTRACTStimulated Raman scattering (SRS) microscopy has gained popularity in recent years due to its linearity to molecule concentration and laser intensity, and to the lack of the nonresonant background that affects its analogous technique, coherent anti-Stokes Raman scattering. However, SRS is no...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Advances in Physics: X |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23746149.2023.2176258 |
| _version_ | 1827789535939919872 |
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| author | Luca Genchi Sergey P. Laptenok Carlo Liberale |
| author_facet | Luca Genchi Sergey P. Laptenok Carlo Liberale |
| author_sort | Luca Genchi |
| collection | DOAJ |
| description | ABSTRACTStimulated Raman scattering (SRS) microscopy has gained popularity in recent years due to its linearity to molecule concentration and laser intensity, and to the lack of the nonresonant background that affects its analogous technique, coherent anti-Stokes Raman scattering. However, SRS is not a background-free technique. In fact, there are other optical processes – nonlinear transient scattering and nonlinear transient absorption – that can be detrimental to the contrast and sensitivity of SRS microscopy. In this review, we provide a description of these competing optical processes and present an up-to-date description of current solutions to minimize their effect on SRS measurements. |
| first_indexed | 2024-03-11T17:20:41Z |
| format | Article |
| id | doaj.art-8d87ad1dbc8147d6a175dcad9df5d2c0 |
| institution | Directory Open Access Journal |
| issn | 2374-6149 |
| language | English |
| last_indexed | 2024-03-11T17:20:41Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Advances in Physics: X |
| spelling | doaj.art-8d87ad1dbc8147d6a175dcad9df5d2c02023-10-19T13:03:27ZengTaylor & Francis GroupAdvances in Physics: X2374-61492023-12-018110.1080/23746149.2023.2176258Background signals in stimulated Raman scattering microscopy and current solutions to avoid themLuca Genchi0Sergey P. Laptenok1Carlo Liberale2Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaBiological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaBiological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaABSTRACTStimulated Raman scattering (SRS) microscopy has gained popularity in recent years due to its linearity to molecule concentration and laser intensity, and to the lack of the nonresonant background that affects its analogous technique, coherent anti-Stokes Raman scattering. However, SRS is not a background-free technique. In fact, there are other optical processes – nonlinear transient scattering and nonlinear transient absorption – that can be detrimental to the contrast and sensitivity of SRS microscopy. In this review, we provide a description of these competing optical processes and present an up-to-date description of current solutions to minimize their effect on SRS measurements.https://www.tandfonline.com/doi/10.1080/23746149.2023.2176258Coherent Raman scattering microscopystimulated Raman scatteringlabel-free microscopymulti-photon microscopy |
| spellingShingle | Luca Genchi Sergey P. Laptenok Carlo Liberale Background signals in stimulated Raman scattering microscopy and current solutions to avoid them Advances in Physics: X Coherent Raman scattering microscopy stimulated Raman scattering label-free microscopy multi-photon microscopy |
| title | Background signals in stimulated Raman scattering microscopy and current solutions to avoid them |
| title_full | Background signals in stimulated Raman scattering microscopy and current solutions to avoid them |
| title_fullStr | Background signals in stimulated Raman scattering microscopy and current solutions to avoid them |
| title_full_unstemmed | Background signals in stimulated Raman scattering microscopy and current solutions to avoid them |
| title_short | Background signals in stimulated Raman scattering microscopy and current solutions to avoid them |
| title_sort | background signals in stimulated raman scattering microscopy and current solutions to avoid them |
| topic | Coherent Raman scattering microscopy stimulated Raman scattering label-free microscopy multi-photon microscopy |
| url | https://www.tandfonline.com/doi/10.1080/23746149.2023.2176258 |
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