SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’
Lily (<i>Lilium</i> spp.) is an important cut flower around the world. Flower senescence in lilies is characterized by the wilting and abscission of tepals, which results in a decrease in flower quality and huge economic loss. However, the mechanism underlying flower senescence in lilies...
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MDPI AG
2021-06-01
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| author | Jing Luo Ruirui Li Xintong Xu Hairui Niu Yujie Zhang Caiyun Wang |
| author_facet | Jing Luo Ruirui Li Xintong Xu Hairui Niu Yujie Zhang Caiyun Wang |
| author_sort | Jing Luo |
| collection | DOAJ |
| description | Lily (<i>Lilium</i> spp.) is an important cut flower around the world. Flower senescence in lilies is characterized by the wilting and abscission of tepals, which results in a decrease in flower quality and huge economic loss. However, the mechanism underlying flower senescence in lilies is largely unknown. In this study, single-molecule, real-time (SMRT) and Illumina sequencing were carried out in <i>L</i><i>. oriental</i> ‘Siberia’. Sequencing yielded 73,218 non-redundant transcripts, with an N50 of 3792 bp. These data were further integrated with three published transcriptomes through cogent analysis, which yielded 62,960 transcripts, with an increase in N50 of 3935 bp. Analysis of differentially expressed genes showed that 319 transcription factors were highly upregulated during flower senescence. The expression of twelve <i>NAC</i> genes and eleven senescence-associated genes (<i>SAGs</i>) showed that <i>LoNAC29</i> and <i>LoSAG39</i> were highly expressed in senescent flowers. Transient overexpression of <i>LoNAC29</i> and <i>LoSAG39</i> in tepals of lily notably accelerated flower senescence, and the promoter activity of <i>LoSAG39</i> was strongly induced by <i>LoNAC29</i>. This work supported new evidence for the molecular mechanism of flower senescence and provided better sequence data for further study in lilies. |
| first_indexed | 2024-03-10T10:40:08Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T10:40:08Z |
| publishDate | 2021-06-01 |
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| spelling | doaj.art-8d8a312d66294d8aae5849d9e1efeed12023-11-21T22:59:17ZengMDPI AGGenes2073-44252021-06-0112686910.3390/genes12060869SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’Jing Luo0Ruirui Li1Xintong Xu2Hairui Niu3Yujie Zhang4Caiyun Wang5Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaLily (<i>Lilium</i> spp.) is an important cut flower around the world. Flower senescence in lilies is characterized by the wilting and abscission of tepals, which results in a decrease in flower quality and huge economic loss. However, the mechanism underlying flower senescence in lilies is largely unknown. In this study, single-molecule, real-time (SMRT) and Illumina sequencing were carried out in <i>L</i><i>. oriental</i> ‘Siberia’. Sequencing yielded 73,218 non-redundant transcripts, with an N50 of 3792 bp. These data were further integrated with three published transcriptomes through cogent analysis, which yielded 62,960 transcripts, with an increase in N50 of 3935 bp. Analysis of differentially expressed genes showed that 319 transcription factors were highly upregulated during flower senescence. The expression of twelve <i>NAC</i> genes and eleven senescence-associated genes (<i>SAGs</i>) showed that <i>LoNAC29</i> and <i>LoSAG39</i> were highly expressed in senescent flowers. Transient overexpression of <i>LoNAC29</i> and <i>LoSAG39</i> in tepals of lily notably accelerated flower senescence, and the promoter activity of <i>LoSAG39</i> was strongly induced by <i>LoNAC29</i>. This work supported new evidence for the molecular mechanism of flower senescence and provided better sequence data for further study in lilies.https://www.mdpi.com/2073-4425/12/6/869<i>Lilium</i> spp.flower senescenceSMRT sequencingsenescence-associated geneNAC |
| spellingShingle | Jing Luo Ruirui Li Xintong Xu Hairui Niu Yujie Zhang Caiyun Wang SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’ Genes <i>Lilium</i> spp. flower senescence SMRT sequencing senescence-associated gene NAC |
| title | SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’ |
| title_full | SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’ |
| title_fullStr | SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’ |
| title_full_unstemmed | SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’ |
| title_short | SMRT and Illumina RNA Sequencing and Characterization of a Key <i>NAC</i> Gene <i>LoNAC29</i> during the Flower Senescence in <i>Lilium oriental</i> ‘Siberia’ |
| title_sort | smrt and illumina rna sequencing and characterization of a key i nac i gene i lonac29 i during the flower senescence in i lilium oriental i siberia |
| topic | <i>Lilium</i> spp. flower senescence SMRT sequencing senescence-associated gene NAC |
| url | https://www.mdpi.com/2073-4425/12/6/869 |
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