Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide.
The aim of this study was to produce epithelial attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of epithelial sealing at the p...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5065151?pdf=render |
| _version_ | 1831659550522998784 |
|---|---|
| author | Shiho Sugawara Masahiko Maeno Cliff Lee Shigemi Nagai David M Kim John Da Silva Masazumi Nagai Hisatomo Kondo |
| author_facet | Shiho Sugawara Masahiko Maeno Cliff Lee Shigemi Nagai David M Kim John Da Silva Masazumi Nagai Hisatomo Kondo |
| author_sort | Shiho Sugawara |
| collection | DOAJ |
| description | The aim of this study was to produce epithelial attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of epithelial sealing at the peri-implant collar. Histologically, epithelial sealing is recognized as the attachment of the basement membrane (BM). BM-attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment. We then hypothesized that epithelial BM-attachment was produced when titanium surface was modified to allow platelet aggregation. Titanium surfaces were coated with a protease activated receptor 4-activating peptide (PAR4-AP). PAR4-AP coating yielded rapid aggregation of platelets on the titanium surface. Platelet aggregates released robust amount of epithelial chemoattractants (IGF-I, TGF-β) and growth factors (EGF, VEGF) on the titanium surface. Human gingival epithelial cells, when they were co-cultured on the platelet aggregates, successfully attached to the PAR4-AP coated titanium surface with spread laminin5 positive BM and consecutive staining of the epithelial tight junction component ZO1, indicating the formation of complete epithelial sheet. These in-vitro results indicate the establishment of epithelial BM-attachment to the titanium surface. |
| first_indexed | 2024-12-19T18:00:45Z |
| format | Article |
| id | doaj.art-8d8bd35522294d8892d4b228c14c3369 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T18:00:45Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8d8bd35522294d8892d4b228c14c33692022-12-21T20:11:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011110e016469310.1371/journal.pone.0164693Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide.Shiho SugawaraMasahiko MaenoCliff LeeShigemi NagaiDavid M KimJohn Da SilvaMasazumi NagaiHisatomo KondoThe aim of this study was to produce epithelial attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of epithelial sealing at the peri-implant collar. Histologically, epithelial sealing is recognized as the attachment of the basement membrane (BM). BM-attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment. We then hypothesized that epithelial BM-attachment was produced when titanium surface was modified to allow platelet aggregation. Titanium surfaces were coated with a protease activated receptor 4-activating peptide (PAR4-AP). PAR4-AP coating yielded rapid aggregation of platelets on the titanium surface. Platelet aggregates released robust amount of epithelial chemoattractants (IGF-I, TGF-β) and growth factors (EGF, VEGF) on the titanium surface. Human gingival epithelial cells, when they were co-cultured on the platelet aggregates, successfully attached to the PAR4-AP coated titanium surface with spread laminin5 positive BM and consecutive staining of the epithelial tight junction component ZO1, indicating the formation of complete epithelial sheet. These in-vitro results indicate the establishment of epithelial BM-attachment to the titanium surface.http://europepmc.org/articles/PMC5065151?pdf=render |
| spellingShingle | Shiho Sugawara Masahiko Maeno Cliff Lee Shigemi Nagai David M Kim John Da Silva Masazumi Nagai Hisatomo Kondo Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide. PLoS ONE |
| title | Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide. |
| title_full | Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide. |
| title_fullStr | Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide. |
| title_full_unstemmed | Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide. |
| title_short | Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide. |
| title_sort | establishment of epithelial attachment on titanium surface coated with platelet activating peptide |
| url | http://europepmc.org/articles/PMC5065151?pdf=render |
| work_keys_str_mv | AT shihosugawara establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT masahikomaeno establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT clifflee establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT shigeminagai establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT davidmkim establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT johndasilva establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT masazuminagai establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide AT hisatomokondo establishmentofepithelialattachmentontitaniumsurfacecoatedwithplateletactivatingpeptide |