Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars
Since artificial insemination is common practice in pig breeding, the quality and persistence of the semen are decisive for the usability of individual boars. In the current study, genome-wide association analyses were performed to investigate the genetic variability underlying phenotypic variations...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/15/3/382 |
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| author | Henry Reyer Ibrahim Abou-Soliman Martin Schulze Hubert Henne Norbert Reinsch Jennifer Schoen Klaus Wimmers |
| author_facet | Henry Reyer Ibrahim Abou-Soliman Martin Schulze Hubert Henne Norbert Reinsch Jennifer Schoen Klaus Wimmers |
| author_sort | Henry Reyer |
| collection | DOAJ |
| description | Since artificial insemination is common practice in pig breeding, the quality and persistence of the semen are decisive for the usability of individual boars. In the current study, genome-wide association analyses were performed to investigate the genetic variability underlying phenotypic variations in semen characteristics. These traits comprise sperm morphology and sperm motility under different temporal and thermal storage conditions, in addition to standard semen quality parameters. Two consecutive samples of the fourth and fifth ejaculates from the same boar were comprehensively analyzed in a genotyped Piétrain boar population. A total of 13 genomic regions on different chromosomes were identified that contain single-nucleotide polymorphisms significantly associated with these traits. Subsequent analysis of the genomic regions revealed candidate genes described to be involved in spermatogenesis, such as <i>FOXL3</i>, <i>GPER1</i>, <i>PDGFA</i>, <i>PRKAR1B</i>, <i>SNRK</i>, <i>SUN1</i>, and <i>TSPO</i>, and sperm motility, including <i>ARRDC4</i>, <i>CEP78</i>, <i>DNAAF5</i>, and <i>GPER1</i>. Some of these genes were also associated with male fertility or infertility in mammals (e.g., <i>CEP78</i>, <i>GPER1</i>). The analyses based on these laboriously determined and valuable phenotypes contribute to a better understanding of the genetic background of male fertility traits in pigs and could prospectively contribute to the improvement of sperm quality through breeding approaches. |
| first_indexed | 2024-04-24T18:14:52Z |
| format | Article |
| id | doaj.art-8d8bf80ebc214cf5b1accbf151e88c41 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-04-24T18:14:52Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-8d8bf80ebc214cf5b1accbf151e88c412024-03-27T13:43:15ZengMDPI AGGenes2073-44252024-03-0115338210.3390/genes15030382Genome-Wide Association Analysis of Semen Characteristics in Piétrain BoarsHenry Reyer0Ibrahim Abou-Soliman1Martin Schulze2Hubert Henne3Norbert Reinsch4Jennifer Schoen5Klaus Wimmers6Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, GermanyInstitute for Reproduction of Farm Animals Schönow, 16321 Bernau, GermanyBHZP GmbH, 21368 Dahlenburg-Ellringen, GermanyResearch Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, GermanyLeibniz Institute for Zoo and Wildlife Research (IZW), 10315 Berlin, GermanyResearch Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, GermanySince artificial insemination is common practice in pig breeding, the quality and persistence of the semen are decisive for the usability of individual boars. In the current study, genome-wide association analyses were performed to investigate the genetic variability underlying phenotypic variations in semen characteristics. These traits comprise sperm morphology and sperm motility under different temporal and thermal storage conditions, in addition to standard semen quality parameters. Two consecutive samples of the fourth and fifth ejaculates from the same boar were comprehensively analyzed in a genotyped Piétrain boar population. A total of 13 genomic regions on different chromosomes were identified that contain single-nucleotide polymorphisms significantly associated with these traits. Subsequent analysis of the genomic regions revealed candidate genes described to be involved in spermatogenesis, such as <i>FOXL3</i>, <i>GPER1</i>, <i>PDGFA</i>, <i>PRKAR1B</i>, <i>SNRK</i>, <i>SUN1</i>, and <i>TSPO</i>, and sperm motility, including <i>ARRDC4</i>, <i>CEP78</i>, <i>DNAAF5</i>, and <i>GPER1</i>. Some of these genes were also associated with male fertility or infertility in mammals (e.g., <i>CEP78</i>, <i>GPER1</i>). The analyses based on these laboriously determined and valuable phenotypes contribute to a better understanding of the genetic background of male fertility traits in pigs and could prospectively contribute to the improvement of sperm quality through breeding approaches.https://www.mdpi.com/2073-4425/15/3/382Piétrain pigsperm morphologysperm motilityspermatogenesisGWAS |
| spellingShingle | Henry Reyer Ibrahim Abou-Soliman Martin Schulze Hubert Henne Norbert Reinsch Jennifer Schoen Klaus Wimmers Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars Genes Piétrain pig sperm morphology sperm motility spermatogenesis GWAS |
| title | Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars |
| title_full | Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars |
| title_fullStr | Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars |
| title_full_unstemmed | Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars |
| title_short | Genome-Wide Association Analysis of Semen Characteristics in Piétrain Boars |
| title_sort | genome wide association analysis of semen characteristics in pietrain boars |
| topic | Piétrain pig sperm morphology sperm motility spermatogenesis GWAS |
| url | https://www.mdpi.com/2073-4425/15/3/382 |
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