Viscoelastic Capillary Flow Cytometry
A compact microfluidic flow cytometer is demonstrated, comprising viscoelastic flow focusing in fused silica capillaries and a fiber optical interface. Viscoelastic flow focusing enables simple device design and operation with a single‐inlet/outlet fluidic configuration. Fused silica capillaries wit...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2023-02-01
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| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202200137 |
| _version_ | 1811169434340950016 |
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| author | Murat Serhatlioglu Emil Alstrup Jensen Maria Niora Anne Todsen Hansen Christian Friberg Nielsen Michelle Maria Theresia Jansman Leticia Hosta-Rigau Morten Hanefeld Dziegiel Kirstine Berg-Sørensen Ian David Hickson Anders Kristensen |
| author_facet | Murat Serhatlioglu Emil Alstrup Jensen Maria Niora Anne Todsen Hansen Christian Friberg Nielsen Michelle Maria Theresia Jansman Leticia Hosta-Rigau Morten Hanefeld Dziegiel Kirstine Berg-Sørensen Ian David Hickson Anders Kristensen |
| author_sort | Murat Serhatlioglu |
| collection | DOAJ |
| description | A compact microfluidic flow cytometer is demonstrated, comprising viscoelastic flow focusing in fused silica capillaries and a fiber optical interface. Viscoelastic flow focusing enables simple device design and operation with a single‐inlet/outlet fluidic configuration. Fused silica capillaries with different inner diameters are effortlessly interchanged to eliminate blockage ratio limitations and enable single‐train particle focusing for a wide range of particle sizes and geometries. The compact system is mounted on an inverted microscope for easy integration with optical imaging and other optofluidic modalities, such as optical trapping and particle sorting. A real‐time cytometric analysis of three channels, forward scattering, side scattering, and fluorescence detection, is performed on LABVIEW. A throughput of 3500 events s−1 is performed on particles of sizes ranging from 2 to 20 μm, using capillaries of different inner diameters ranging from 30 to 75 μm. The outer diameter of all capillaries is identical to the cladding diameter of the applied optical fibers. This enables easy exchange and precise optical alignment of fibers and capillaries on a microfabricated jig. The performance of the microfluidic flow cytometer is benchmarked using polystyrene calibration beads, poly(lactic‐co‐glycolic acid) particles, erythrocytes, THP‐1 leukemic monocytes, and human metaphase chromosomes. |
| first_indexed | 2024-04-10T16:43:24Z |
| format | Article |
| id | doaj.art-8d8e42562cce4281b8438a40dd47f490 |
| institution | Directory Open Access Journal |
| issn | 2699-9307 |
| language | English |
| last_indexed | 2024-04-10T16:43:24Z |
| publishDate | 2023-02-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj.art-8d8e42562cce4281b8438a40dd47f4902023-02-08T05:18:55ZengWiley-VCHAdvanced NanoBiomed Research2699-93072023-02-0132n/an/a10.1002/anbr.202200137Viscoelastic Capillary Flow CytometryMurat Serhatlioglu0Emil Alstrup Jensen1Maria Niora2Anne Todsen Hansen3Christian Friberg Nielsen4Michelle Maria Theresia Jansman5Leticia Hosta-Rigau6Morten Hanefeld Dziegiel7Kirstine Berg-Sørensen8Ian David Hickson9Anders Kristensen10Department of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkDepartment of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkDepartment of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkDepartment of Clinical Immunology University of Copenhagen Blegdamsvej 9 2100 København Ø DenmarkCenter for Chromosome Stability Department of Cellular and Molecular Medicine University of Copenhagen 2200 København N. DenmarkDepartment of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkDepartment of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkDepartment of Clinical Immunology University of Copenhagen Blegdamsvej 9 2100 København Ø DenmarkDepartment of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkCenter for Chromosome Stability Department of Cellular and Molecular Medicine University of Copenhagen 2200 København N. DenmarkDepartment of Health Technology Technical University of Denmark Ørsteds Plads Building 345C 2800 Kongens Lyngby DenmarkA compact microfluidic flow cytometer is demonstrated, comprising viscoelastic flow focusing in fused silica capillaries and a fiber optical interface. Viscoelastic flow focusing enables simple device design and operation with a single‐inlet/outlet fluidic configuration. Fused silica capillaries with different inner diameters are effortlessly interchanged to eliminate blockage ratio limitations and enable single‐train particle focusing for a wide range of particle sizes and geometries. The compact system is mounted on an inverted microscope for easy integration with optical imaging and other optofluidic modalities, such as optical trapping and particle sorting. A real‐time cytometric analysis of three channels, forward scattering, side scattering, and fluorescence detection, is performed on LABVIEW. A throughput of 3500 events s−1 is performed on particles of sizes ranging from 2 to 20 μm, using capillaries of different inner diameters ranging from 30 to 75 μm. The outer diameter of all capillaries is identical to the cladding diameter of the applied optical fibers. This enables easy exchange and precise optical alignment of fibers and capillaries on a microfabricated jig. The performance of the microfluidic flow cytometer is benchmarked using polystyrene calibration beads, poly(lactic‐co‐glycolic acid) particles, erythrocytes, THP‐1 leukemic monocytes, and human metaphase chromosomes.https://doi.org/10.1002/anbr.202200137blood cellscapillarychromosomesflow cytometrymicrofluidicsoptofluidics |
| spellingShingle | Murat Serhatlioglu Emil Alstrup Jensen Maria Niora Anne Todsen Hansen Christian Friberg Nielsen Michelle Maria Theresia Jansman Leticia Hosta-Rigau Morten Hanefeld Dziegiel Kirstine Berg-Sørensen Ian David Hickson Anders Kristensen Viscoelastic Capillary Flow Cytometry Advanced NanoBiomed Research blood cells capillary chromosomes flow cytometry microfluidics optofluidics |
| title | Viscoelastic Capillary Flow Cytometry |
| title_full | Viscoelastic Capillary Flow Cytometry |
| title_fullStr | Viscoelastic Capillary Flow Cytometry |
| title_full_unstemmed | Viscoelastic Capillary Flow Cytometry |
| title_short | Viscoelastic Capillary Flow Cytometry |
| title_sort | viscoelastic capillary flow cytometry |
| topic | blood cells capillary chromosomes flow cytometry microfluidics optofluidics |
| url | https://doi.org/10.1002/anbr.202200137 |
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