Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo
Osteoarthritis (OA) is a degenerative disease found in middle-aged and elderly people, which seriously affects their quality of life. The anti-inflammatory and anti-apoptosis pharmacological effects of salvianolic acid A (SAA) have been shown in many studies. In this study, we intended to explore th...
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| Format: | Article |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.00682/full |
| _version_ | 1818030092086935552 |
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| author | Yifan Wu Zhanghong Wang Zeng Lin Xin Fu Jingdi Zhan Kehe Yu |
| author_facet | Yifan Wu Zhanghong Wang Zeng Lin Xin Fu Jingdi Zhan Kehe Yu |
| author_sort | Yifan Wu |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a degenerative disease found in middle-aged and elderly people, which seriously affects their quality of life. The anti-inflammatory and anti-apoptosis pharmacological effects of salvianolic acid A (SAA) have been shown in many studies. In this study, we intended to explore the anti-inflammatory and anti-apoptotic effects of SAA in OA. We evaluated the expression of pro-inflammatory mediators and cartilage matrix catabolic enzymes in chondrocytes by ELISA, Griess reaction, immunofluorescence, and Western blot, which includes nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), MMPs (MMP-3, MMP-13), and ADAMTS-5. Bax, Bcl-2, and cleaved caspase-3 were also measured by Western blot methods. The results of this experiment in vitro showed that SAA not only inhibited the production of inflammatory mediators induced by IL-1β and the loss of cartilage matrix but also reduced the apoptosis of mouse chondrocytes induced by IL-1β. According to the results of immunofluorescence and Western blot, SAA inhibited the activation of the NF-κB pathway and MAPK pathway. The results of these in vitro experiments revealed for the first time that SAA down-regulated the production of inflammatory mediators and inhibited the apoptosis of mouse chondrocytes and the degradation of extracellular matrix (ECM), which may be attributed to the inhibition of the activation of NF-κB and MAPK signaling pathways. In the in vivo experiments, 45 mice were randomly divided among three groups (the sham group, OA group, and OA + SAA group). The results of animal experiments showed that SAA treatment for eight consecutive weeks inhibited further deterioration of OA. These results demonstrate that SAA plays an active therapeutic role in the development of OA. |
| first_indexed | 2024-12-10T05:30:05Z |
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| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-10T05:30:05Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-8d8ffccdcde843e6aa90adb63e6fae742022-12-22T02:00:35ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-06-011110.3389/fphar.2020.00682503001Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In VivoYifan Wu0Zhanghong Wang1Zeng Lin2Xin Fu3Jingdi Zhan4Kehe Yu5Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Anesthesiology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaOsteoarthritis (OA) is a degenerative disease found in middle-aged and elderly people, which seriously affects their quality of life. The anti-inflammatory and anti-apoptosis pharmacological effects of salvianolic acid A (SAA) have been shown in many studies. In this study, we intended to explore the anti-inflammatory and anti-apoptotic effects of SAA in OA. We evaluated the expression of pro-inflammatory mediators and cartilage matrix catabolic enzymes in chondrocytes by ELISA, Griess reaction, immunofluorescence, and Western blot, which includes nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), MMPs (MMP-3, MMP-13), and ADAMTS-5. Bax, Bcl-2, and cleaved caspase-3 were also measured by Western blot methods. The results of this experiment in vitro showed that SAA not only inhibited the production of inflammatory mediators induced by IL-1β and the loss of cartilage matrix but also reduced the apoptosis of mouse chondrocytes induced by IL-1β. According to the results of immunofluorescence and Western blot, SAA inhibited the activation of the NF-κB pathway and MAPK pathway. The results of these in vitro experiments revealed for the first time that SAA down-regulated the production of inflammatory mediators and inhibited the apoptosis of mouse chondrocytes and the degradation of extracellular matrix (ECM), which may be attributed to the inhibition of the activation of NF-κB and MAPK signaling pathways. In the in vivo experiments, 45 mice were randomly divided among three groups (the sham group, OA group, and OA + SAA group). The results of animal experiments showed that SAA treatment for eight consecutive weeks inhibited further deterioration of OA. These results demonstrate that SAA plays an active therapeutic role in the development of OA.https://www.frontiersin.org/article/10.3389/fphar.2020.00682/fullsalvianolic acid AchondrocytesosteoarthritisinflammatoryapoptosisMAPK |
| spellingShingle | Yifan Wu Zhanghong Wang Zeng Lin Xin Fu Jingdi Zhan Kehe Yu Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo Frontiers in Pharmacology salvianolic acid A chondrocytes osteoarthritis inflammatory apoptosis MAPK |
| title | Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo |
| title_full | Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo |
| title_fullStr | Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo |
| title_full_unstemmed | Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo |
| title_short | Salvianolic Acid A Has Anti-Osteoarthritis Effect In Vitro and In Vivo |
| title_sort | salvianolic acid a has anti osteoarthritis effect in vitro and in vivo |
| topic | salvianolic acid A chondrocytes osteoarthritis inflammatory apoptosis MAPK |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.00682/full |
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