Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats
Our aim was to examine whether vanadium (IV) corrects alterations in zinc, copper and manganese homeostasis, observed in streptozotocin-induced hyperglycemic rats, and whether such changes are related to divalent metal transporter 1 (DMT1) mRNA expression, and antioxidant and proinflammatory paramet...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/6/814 |
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| author | Cristina Sánchez-González Laura Moreno Pilar Aranda María Montes-Bayón Juan Llopis Lorenzo Rivas-García |
| author_facet | Cristina Sánchez-González Laura Moreno Pilar Aranda María Montes-Bayón Juan Llopis Lorenzo Rivas-García |
| author_sort | Cristina Sánchez-González |
| collection | DOAJ |
| description | Our aim was to examine whether vanadium (IV) corrects alterations in zinc, copper and manganese homeostasis, observed in streptozotocin-induced hyperglycemic rats, and whether such changes are related to divalent metal transporter 1 (DMT1) mRNA expression, and antioxidant and proinflammatory parameters. Four groups of Wistar rats were examined: control; hyperglycemic (H); hyperglycemic treated with 1 mg V/day (HV); and hyperglycemic treated with 3 mg V/day (HVH). Vanadium was supplied in drinking water as bis(maltolato)oxovanadium(IV) for five weeks. Zinc, copper and manganese were measured in food, excreta, serum and tissues. DMT1 mRNA expression was quantified in the liver. Hyperglycemic rats showed increased Zn and Cu absorption and content in the liver, serum, kidneys and femurs; DMT1 expression also increased (<i>p</i> < 0.05 in all cases). HV rats showed no changes compared to H rats other than decreased DMT1 expression (<i>p</i> < 0.05). In the HVH group, decreased absorption and tissular content of studied elements (<i>p</i> < 0.05 in all cases) and DMT1 expression compared to H (<i>p</i> < 0.05) were observed. Liver zinc, copper and manganese content correlated positively with glutathione peroxidase activity and negatively with catalase activity (<i>p</i> < 0.05 in both cases). In conclusion, treatment with 3 mg V/d reverted the alterations in zinc and copper homeostasis caused by hyperglycemia, possibly facilitated by decreased DMT1 expression. |
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| spelling | doaj.art-8d90e0066be549db964e12db199e2f6c2023-11-23T15:38:39ZengMDPI AGBiology2079-77372022-05-0111681410.3390/biology11060814Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic RatsCristina Sánchez-González0Laura Moreno1Pilar Aranda2María Montes-Bayón3Juan Llopis4Lorenzo Rivas-García5Department of Physiology, Institute of Nutrition and Food Technology ’’José Mataix“, Biomedical Research Centre, University of Granada, Avda. Del Conocimiento s.n., 18100 Armilla, SpainDepartment of Physiology, Institute of Nutrition and Food Technology ’’José Mataix“, Biomedical Research Centre, University of Granada, Avda. Del Conocimiento s.n., 18100 Armilla, SpainDepartment of Physiology, Institute of Nutrition and Food Technology ’’José Mataix“, Biomedical Research Centre, University of Granada, Avda. Del Conocimiento s.n., 18100 Armilla, SpainDepartment of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33007 Oviedo, SpainDepartment of Physiology, Institute of Nutrition and Food Technology ’’José Mataix“, Biomedical Research Centre, University of Granada, Avda. Del Conocimiento s.n., 18100 Armilla, SpainDepartment of Physiology, Institute of Nutrition and Food Technology ’’José Mataix“, Biomedical Research Centre, University of Granada, Avda. Del Conocimiento s.n., 18100 Armilla, SpainOur aim was to examine whether vanadium (IV) corrects alterations in zinc, copper and manganese homeostasis, observed in streptozotocin-induced hyperglycemic rats, and whether such changes are related to divalent metal transporter 1 (DMT1) mRNA expression, and antioxidant and proinflammatory parameters. Four groups of Wistar rats were examined: control; hyperglycemic (H); hyperglycemic treated with 1 mg V/day (HV); and hyperglycemic treated with 3 mg V/day (HVH). Vanadium was supplied in drinking water as bis(maltolato)oxovanadium(IV) for five weeks. Zinc, copper and manganese were measured in food, excreta, serum and tissues. DMT1 mRNA expression was quantified in the liver. Hyperglycemic rats showed increased Zn and Cu absorption and content in the liver, serum, kidneys and femurs; DMT1 expression also increased (<i>p</i> < 0.05 in all cases). HV rats showed no changes compared to H rats other than decreased DMT1 expression (<i>p</i> < 0.05). In the HVH group, decreased absorption and tissular content of studied elements (<i>p</i> < 0.05 in all cases) and DMT1 expression compared to H (<i>p</i> < 0.05) were observed. Liver zinc, copper and manganese content correlated positively with glutathione peroxidase activity and negatively with catalase activity (<i>p</i> < 0.05 in both cases). In conclusion, treatment with 3 mg V/d reverted the alterations in zinc and copper homeostasis caused by hyperglycemia, possibly facilitated by decreased DMT1 expression.https://www.mdpi.com/2079-7737/11/6/814mineral metabolismDMT1nutrition |
| spellingShingle | Cristina Sánchez-González Laura Moreno Pilar Aranda María Montes-Bayón Juan Llopis Lorenzo Rivas-García Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats Biology mineral metabolism DMT1 nutrition |
| title | Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats |
| title_full | Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats |
| title_fullStr | Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats |
| title_full_unstemmed | Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats |
| title_short | Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats |
| title_sort | effect of bis maltolato oxovanadium iv on zinc copper and manganese homeostasis and dmt1 mrna expression in streptozotocin induced hyperglycemic rats |
| topic | mineral metabolism DMT1 nutrition |
| url | https://www.mdpi.com/2079-7737/11/6/814 |
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