Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression
As an endocrine organ, skeletal muscle secretes multiple myokines. Myonectin, a myokine, participates in lipid and glucose metabolism. To explore the functions of myonectin in teleosts, the myonectin gene was cloned from the brain tissue of grass carp. The open reading frame (ORF) of isolated myonec...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422003027 |
| _version_ | 1818019948748865536 |
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| author | Guokun Yang Xiaomin Liang Yanle Jiang Chengquan Li Jihong Hu Yanmin Zhang Xindang Zhang Xulu Chang Wenpan Hu Keke Li Xiaolin Meng |
| author_facet | Guokun Yang Xiaomin Liang Yanle Jiang Chengquan Li Jihong Hu Yanmin Zhang Xindang Zhang Xulu Chang Wenpan Hu Keke Li Xiaolin Meng |
| author_sort | Guokun Yang |
| collection | DOAJ |
| description | As an endocrine organ, skeletal muscle secretes multiple myokines. Myonectin, a myokine, participates in lipid and glucose metabolism. To explore the functions of myonectin in teleosts, the myonectin gene was cloned from the brain tissue of grass carp. The open reading frame (ORF) of isolated myonectin was 813 bp, encoding 270 amino acids. Grass carp myonectin has four predicted domains: signal peptide, N-terminal domain (NTD), collagen protein domain, and C1q/TNF-like domain. In addition, four predicted glycosylation sites and four conserved cysteine residues were located in grass carp myonectin. Myonectin mRNA was detected in multiple tissues of grass carp and was highly expressed in the brain region, heart and kidney. In the oral glucose tolerance test (OGTT), myonectin mRNA levels were significantly induced in the kidney, hypothalamus, muscle and brain after treatment with glucose. In the fast and refeeding experiment, the serum glucose level was decreased in the fast group. However, serum glucose levels were increased in the refeeding group. The myonectin mRNA levels in the kidney, heart, brain and liver were significantly reduced in the fast groups. Moreover, the myonectin expression was markedly increased in the refeeding groups. In grass carp primary hepatocytes, myonectin mRNA levels were induced by treatment with glucose and oleic acid for 12 or 24 h. Myonectin expression was dramatically promoted by treatment with insulin. Moreover, myonectin expression was markedly decreased with glucagon treatment in primary hepatocytes. The results of this study will provide a basis for exploring the roles of grass carp myonectin in the future. |
| first_indexed | 2024-04-14T07:58:36Z |
| format | Article |
| id | doaj.art-8d95e7d124fe45349a8ee719f9018145 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-14T07:58:36Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-8d95e7d124fe45349a8ee719f90181452022-12-22T02:04:57ZengElsevierAquaculture Reports2352-51342022-10-0126101306Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expressionGuokun Yang0Xiaomin Liang1Yanle Jiang2Chengquan Li3Jihong Hu4Yanmin Zhang5Xindang Zhang6Xulu Chang7Wenpan Hu8Keke Li9Xiaolin Meng10College of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaHenan Jinbaihe Biotechnology Co., LTD, Anyang 456171, PR ChinaHenan Jinbaihe Biotechnology Co., LTD, Anyang 456171, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR China; Corresponding author at: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.As an endocrine organ, skeletal muscle secretes multiple myokines. Myonectin, a myokine, participates in lipid and glucose metabolism. To explore the functions of myonectin in teleosts, the myonectin gene was cloned from the brain tissue of grass carp. The open reading frame (ORF) of isolated myonectin was 813 bp, encoding 270 amino acids. Grass carp myonectin has four predicted domains: signal peptide, N-terminal domain (NTD), collagen protein domain, and C1q/TNF-like domain. In addition, four predicted glycosylation sites and four conserved cysteine residues were located in grass carp myonectin. Myonectin mRNA was detected in multiple tissues of grass carp and was highly expressed in the brain region, heart and kidney. In the oral glucose tolerance test (OGTT), myonectin mRNA levels were significantly induced in the kidney, hypothalamus, muscle and brain after treatment with glucose. In the fast and refeeding experiment, the serum glucose level was decreased in the fast group. However, serum glucose levels were increased in the refeeding group. The myonectin mRNA levels in the kidney, heart, brain and liver were significantly reduced in the fast groups. Moreover, the myonectin expression was markedly increased in the refeeding groups. In grass carp primary hepatocytes, myonectin mRNA levels were induced by treatment with glucose and oleic acid for 12 or 24 h. Myonectin expression was dramatically promoted by treatment with insulin. Moreover, myonectin expression was markedly decreased with glucagon treatment in primary hepatocytes. The results of this study will provide a basis for exploring the roles of grass carp myonectin in the future.http://www.sciencedirect.com/science/article/pii/S2352513422003027MyonectinCloneExpressionGrass carp |
| spellingShingle | Guokun Yang Xiaomin Liang Yanle Jiang Chengquan Li Jihong Hu Yanmin Zhang Xindang Zhang Xulu Chang Wenpan Hu Keke Li Xiaolin Meng Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression Aquaculture Reports Myonectin Clone Expression Grass carp |
| title | Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression |
| title_full | Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression |
| title_fullStr | Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression |
| title_full_unstemmed | Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression |
| title_short | Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression |
| title_sort | myonectin in grass carp ctenopharyngodon idella molecular cloning and effect of nutrition state insulin and glucagon on myonectin expression |
| topic | Myonectin Clone Expression Grass carp |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422003027 |
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