BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages.
HLA-B-associated transcript 3 (BAT3), also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. We demonstrate that BAT3 modulates the immune response by regulating the function of macrophages. BAT3 is rele...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3396635?pdf=render |
| _version_ | 1819213212320530432 |
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| author | Ajay Grover Angelo A Izzo |
| author_facet | Ajay Grover Angelo A Izzo |
| author_sort | Ajay Grover |
| collection | DOAJ |
| description | HLA-B-associated transcript 3 (BAT3), also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. We demonstrate that BAT3 modulates the immune response by regulating the function of macrophages. BAT3 is released by macrophages in vitro and it down-regulates nitric oxide and proinflammatory cytokines release in IFN-γ and LPS stimulated macrophages. Furthermore, Mycobacterium tuberculosis-derived protein ESAT-6 (Rv3875) induced transient increase in the expression and release of BAT3 in macrophages. We show that induction of apoptosis by ESAT-6 is dependent on the cleavage of BAT3 by caspase-3 and proteasomal degradation. Our results also indicate that BAT3 regulates ESAT-6-induced apoptosis by interacting with anti-apoptotic protein BCL-2. Taken together, the data suggest that BAT3 plays a role in the early immune response to M. tuberculosis infection and may be a key protein associated with the fate of antigen presenting cells during infection. |
| first_indexed | 2024-12-23T06:55:16Z |
| format | Article |
| id | doaj.art-8d9697756fa1474988a81aee3912b4e2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T06:55:16Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8d9697756fa1474988a81aee3912b4e22022-12-21T17:56:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4083610.1371/journal.pone.0040836BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages.Ajay GroverAngelo A IzzoHLA-B-associated transcript 3 (BAT3), also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. We demonstrate that BAT3 modulates the immune response by regulating the function of macrophages. BAT3 is released by macrophages in vitro and it down-regulates nitric oxide and proinflammatory cytokines release in IFN-γ and LPS stimulated macrophages. Furthermore, Mycobacterium tuberculosis-derived protein ESAT-6 (Rv3875) induced transient increase in the expression and release of BAT3 in macrophages. We show that induction of apoptosis by ESAT-6 is dependent on the cleavage of BAT3 by caspase-3 and proteasomal degradation. Our results also indicate that BAT3 regulates ESAT-6-induced apoptosis by interacting with anti-apoptotic protein BCL-2. Taken together, the data suggest that BAT3 plays a role in the early immune response to M. tuberculosis infection and may be a key protein associated with the fate of antigen presenting cells during infection.http://europepmc.org/articles/PMC3396635?pdf=render |
| spellingShingle | Ajay Grover Angelo A Izzo BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages. PLoS ONE |
| title | BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages. |
| title_full | BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages. |
| title_fullStr | BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages. |
| title_full_unstemmed | BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages. |
| title_short | BAT3 regulates Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis of macrophages. |
| title_sort | bat3 regulates mycobacterium tuberculosis protein esat 6 mediated apoptosis of macrophages |
| url | http://europepmc.org/articles/PMC3396635?pdf=render |
| work_keys_str_mv | AT ajaygrover bat3regulatesmycobacteriumtuberculosisproteinesat6mediatedapoptosisofmacrophages AT angeloaizzo bat3regulatesmycobacteriumtuberculosisproteinesat6mediatedapoptosisofmacrophages |